Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by <i>Actinobacillus succinogenes</i> GXAS137
Sugarcane bagasse (SCB) is an abundant agricultural waste, rich in cellulose and hemicellulose, that could be used as an ideal raw material for succinic acid (SA) production. A two-step chemical pretreatment, involving alkali extraction and alkaline hydrogen peroxide treatment, was utilized to treat...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/10/1/22 |
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| author | Yan Qin Yi Li Ge Liang Naikun Shen Liang Xian Qingyan Wang |
| author_facet | Yan Qin Yi Li Ge Liang Naikun Shen Liang Xian Qingyan Wang |
| author_sort | Yan Qin |
| collection | DOAJ |
| description | Sugarcane bagasse (SCB) is an abundant agricultural waste, rich in cellulose and hemicellulose, that could be used as an ideal raw material for succinic acid (SA) production. A two-step chemical pretreatment, involving alkali extraction and alkaline hydrogen peroxide treatment, was utilized to treat SCB, followed by multi-enzyme hydrolysis to obtain a reducing sugar hydrolysate mainly composed of glucose and xylose. Optimization of the multi-enzyme hydrolysis of pretreated SCB resulted in a final reducing sugar concentration of 78.34 g/L. In order to enhance the bioconversion of SCB to SA and to reduce the production costs, the initial reducing sugar concentration, nitrogen source, and MgCO<sub>3</sub> content were further optimized. The results demonstrated that the inexpensive corn steep liquor powder (CSLP) could be utilized as an alternative nitrogen source to yeast extract for the production of SA; and the optimal concentrations of initial reducing sugar, CSLP, and MgCO<sub>3</sub> were 70 g/L, 18 g/L, and 60 g/L, respectively. When fed-batch fermentation was conducted in a 2 L stirred bioreactor, approximately 72.9 g/L of SA was produced, with a yield of 83.2% and a productivity of 1.40 g/L/h. The high SA concentration, yield, and productivity achieved in this study demonstrate the potential of SCB, an agricultural waste, as a viable alternative substrate for <i>Actinobacillus succinogenes</i> GXAS137 to produce SA. This lays a solid foundation for the resource utilization of agricultural waste and cost-effective industrial-scale production of SA in the future. |
| first_indexed | 2024-03-08T10:57:24Z |
| format | Article |
| id | doaj.art-9785fd321f4b497c8f468bb33561c875 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-08T10:57:24Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-9785fd321f4b497c8f468bb33561c8752024-01-26T16:23:41ZengMDPI AGFermentation2311-56372023-12-011012210.3390/fermentation10010022Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by <i>Actinobacillus succinogenes</i> GXAS137Yan Qin0Yi Li1Ge Liang2Naikun Shen3Liang Xian4Qingyan Wang5National Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, 98 Daling Road, Nanning 530007, ChinaNational Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, 98 Daling Road, Nanning 530007, ChinaNational Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, 98 Daling Road, Nanning 530007, ChinaSchool of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning 530006, ChinaNational Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, 98 Daling Road, Nanning 530007, ChinaNational Key Laboratory of Non-Food Biomass Energy Technology, Guangxi Biomass Engineering Technology Research Center, Guangxi Academy of Sciences, 98 Daling Road, Nanning 530007, ChinaSugarcane bagasse (SCB) is an abundant agricultural waste, rich in cellulose and hemicellulose, that could be used as an ideal raw material for succinic acid (SA) production. A two-step chemical pretreatment, involving alkali extraction and alkaline hydrogen peroxide treatment, was utilized to treat SCB, followed by multi-enzyme hydrolysis to obtain a reducing sugar hydrolysate mainly composed of glucose and xylose. Optimization of the multi-enzyme hydrolysis of pretreated SCB resulted in a final reducing sugar concentration of 78.34 g/L. In order to enhance the bioconversion of SCB to SA and to reduce the production costs, the initial reducing sugar concentration, nitrogen source, and MgCO<sub>3</sub> content were further optimized. The results demonstrated that the inexpensive corn steep liquor powder (CSLP) could be utilized as an alternative nitrogen source to yeast extract for the production of SA; and the optimal concentrations of initial reducing sugar, CSLP, and MgCO<sub>3</sub> were 70 g/L, 18 g/L, and 60 g/L, respectively. When fed-batch fermentation was conducted in a 2 L stirred bioreactor, approximately 72.9 g/L of SA was produced, with a yield of 83.2% and a productivity of 1.40 g/L/h. The high SA concentration, yield, and productivity achieved in this study demonstrate the potential of SCB, an agricultural waste, as a viable alternative substrate for <i>Actinobacillus succinogenes</i> GXAS137 to produce SA. This lays a solid foundation for the resource utilization of agricultural waste and cost-effective industrial-scale production of SA in the future.https://www.mdpi.com/2311-5637/10/1/22succinic acidsugarcane bagassepretreatmentenzymatic hydrolysiscorn steep liquor powderfermentation |
| spellingShingle | Yan Qin Yi Li Ge Liang Naikun Shen Liang Xian Qingyan Wang Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by <i>Actinobacillus succinogenes</i> GXAS137 Fermentation succinic acid sugarcane bagasse pretreatment enzymatic hydrolysis corn steep liquor powder fermentation |
| title | Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by <i>Actinobacillus succinogenes</i> GXAS137 |
| title_full | Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by <i>Actinobacillus succinogenes</i> GXAS137 |
| title_fullStr | Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by <i>Actinobacillus succinogenes</i> GXAS137 |
| title_full_unstemmed | Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by <i>Actinobacillus succinogenes</i> GXAS137 |
| title_short | Efficient Production of Succinic Acid from Sugarcane Bagasse Hydrolysate by <i>Actinobacillus succinogenes</i> GXAS137 |
| title_sort | efficient production of succinic acid from sugarcane bagasse hydrolysate by i actinobacillus succinogenes i gxas137 |
| topic | succinic acid sugarcane bagasse pretreatment enzymatic hydrolysis corn steep liquor powder fermentation |
| url | https://www.mdpi.com/2311-5637/10/1/22 |
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