Induction of Antioxidant and Heat Shock Protein Responses During Torpor in the Gray Mouse Lemur, Microcebus murinus
A natural tolerance of various environmental stresses is typically supported by various cytoprotective mechanisms that protect macromolecules and promote extended viability. Among these are antioxidant defenses that help to limit damage from reactive oxygen species and chaperones that help to minimi...
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| Format: | Article |
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Oxford University Press
2015-04-01
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| Series: | Genomics, Proteomics & Bioinformatics |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672022915000480 |
| _version_ | 1827160458440736768 |
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| author | Cheng-Wei Wu Kyle K. Biggar Jing Zhang Shannon N. Tessier Fabien Pifferi Martine Perret Kenneth B. Storey |
| author_facet | Cheng-Wei Wu Kyle K. Biggar Jing Zhang Shannon N. Tessier Fabien Pifferi Martine Perret Kenneth B. Storey |
| author_sort | Cheng-Wei Wu |
| collection | DOAJ |
| description | A natural tolerance of various environmental stresses is typically supported by various cytoprotective mechanisms that protect macromolecules and promote extended viability. Among these are antioxidant defenses that help to limit damage from reactive oxygen species and chaperones that help to minimize protein misfolding or unfolding under stress conditions. To understand the molecular mechanisms that act to protect cells during primate torpor, the present study characterizes antioxidant and heat shock protein (HSP) responses in various organs of control (aroused) and torpid gray mouse lemurs, Microcebus murinus. Protein expression of HSP70 and HSP90α was elevated to 1.26 and 1.49 fold, respectively, in brown adipose tissue during torpor as compared with control animals, whereas HSP60 in liver of torpid animals was 1.15 fold of that in control (P < 0.05). Among antioxidant enzymes, protein levels of thioredoxin 1 were elevated to 2.19 fold in white adipose tissue during torpor, whereas Cu–Zn superoxide dismutase 1 levels rose to 1.1 fold in skeletal muscle (P < 0.05). Additionally, total antioxidant capacity was increased to 1.6 fold in liver during torpor (P < 0.05), while remaining unchanged in the five other tissues. Overall, our data suggest that antioxidant and HSP responses are modified in a tissue-specific manner during daily torpor in gray mouse lemurs. Furthermore, our data also show that cytoprotective strategies employed during primate torpor are distinct from the strategies in rodent hibernation as reported in previous studies. |
| first_indexed | 2024-03-08T07:39:14Z |
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| institution | Directory Open Access Journal |
| issn | 1672-0229 |
| language | English |
| last_indexed | 2025-03-21T00:13:21Z |
| publishDate | 2015-04-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Genomics, Proteomics & Bioinformatics |
| spelling | doaj.art-9786ea627bb94413932e734345a9c5772024-08-03T09:43:13ZengOxford University PressGenomics, Proteomics & Bioinformatics1672-02292015-04-0113211912610.1016/j.gpb.2015.03.004Induction of Antioxidant and Heat Shock Protein Responses During Torpor in the Gray Mouse Lemur, Microcebus murinusCheng-Wei Wu0Kyle K. Biggar1Jing Zhang2Shannon N. Tessier3Fabien Pifferi4Martine Perret5Kenneth B. Storey6Institute of Biochemistry & Department of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaInstitute of Biochemistry & Department of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaInstitute of Biochemistry & Department of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaInstitute of Biochemistry & Department of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaUMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle, Brunoy 91800, FranceUMR 7179 Centre National de la Recherche Scientifique, Muséum National d’Histoire Naturelle, Brunoy 91800, FranceInstitute of Biochemistry & Department of Biology, Carleton University, Ottawa, ON K1S 5B6, CanadaA natural tolerance of various environmental stresses is typically supported by various cytoprotective mechanisms that protect macromolecules and promote extended viability. Among these are antioxidant defenses that help to limit damage from reactive oxygen species and chaperones that help to minimize protein misfolding or unfolding under stress conditions. To understand the molecular mechanisms that act to protect cells during primate torpor, the present study characterizes antioxidant and heat shock protein (HSP) responses in various organs of control (aroused) and torpid gray mouse lemurs, Microcebus murinus. Protein expression of HSP70 and HSP90α was elevated to 1.26 and 1.49 fold, respectively, in brown adipose tissue during torpor as compared with control animals, whereas HSP60 in liver of torpid animals was 1.15 fold of that in control (P < 0.05). Among antioxidant enzymes, protein levels of thioredoxin 1 were elevated to 2.19 fold in white adipose tissue during torpor, whereas Cu–Zn superoxide dismutase 1 levels rose to 1.1 fold in skeletal muscle (P < 0.05). Additionally, total antioxidant capacity was increased to 1.6 fold in liver during torpor (P < 0.05), while remaining unchanged in the five other tissues. Overall, our data suggest that antioxidant and HSP responses are modified in a tissue-specific manner during daily torpor in gray mouse lemurs. Furthermore, our data also show that cytoprotective strategies employed during primate torpor are distinct from the strategies in rodent hibernation as reported in previous studies.http://www.sciencedirect.com/science/article/pii/S1672022915000480Heat shock proteinsAntioxidant capacityPrimate hypometabolismStress response |
| spellingShingle | Cheng-Wei Wu Kyle K. Biggar Jing Zhang Shannon N. Tessier Fabien Pifferi Martine Perret Kenneth B. Storey Induction of Antioxidant and Heat Shock Protein Responses During Torpor in the Gray Mouse Lemur, Microcebus murinus Genomics, Proteomics & Bioinformatics Heat shock proteins Antioxidant capacity Primate hypometabolism Stress response |
| title | Induction of Antioxidant and Heat Shock Protein Responses During Torpor in the Gray Mouse Lemur, Microcebus murinus |
| title_full | Induction of Antioxidant and Heat Shock Protein Responses During Torpor in the Gray Mouse Lemur, Microcebus murinus |
| title_fullStr | Induction of Antioxidant and Heat Shock Protein Responses During Torpor in the Gray Mouse Lemur, Microcebus murinus |
| title_full_unstemmed | Induction of Antioxidant and Heat Shock Protein Responses During Torpor in the Gray Mouse Lemur, Microcebus murinus |
| title_short | Induction of Antioxidant and Heat Shock Protein Responses During Torpor in the Gray Mouse Lemur, Microcebus murinus |
| title_sort | induction of antioxidant and heat shock protein responses during torpor in the gray mouse lemur microcebus murinus |
| topic | Heat shock proteins Antioxidant capacity Primate hypometabolism Stress response |
| url | http://www.sciencedirect.com/science/article/pii/S1672022915000480 |
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