Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration.
Pseudomonas aeruginosa, a gram-negative bacterium of clinical importance, forms more robust biofilm during anaerobic respiration, a mode of growth presumed to occur in abnormally thickened mucus layer lining the cystic fibrosis (CF) patient airway. However, molecular basis behind this anaerobiosis-t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3022656?pdf=render |
| _version_ | 1818137897033793536 |
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| author | Mi Young Yoon Kang-Mu Lee Yongjin Park Sang Sun Yoon |
| author_facet | Mi Young Yoon Kang-Mu Lee Yongjin Park Sang Sun Yoon |
| author_sort | Mi Young Yoon |
| collection | DOAJ |
| description | Pseudomonas aeruginosa, a gram-negative bacterium of clinical importance, forms more robust biofilm during anaerobic respiration, a mode of growth presumed to occur in abnormally thickened mucus layer lining the cystic fibrosis (CF) patient airway. However, molecular basis behind this anaerobiosis-triggered robust biofilm formation is not clearly defined yet. Here, we identified a morphological change naturally accompanied by anaerobic respiration in P. aeruginosa and investigated its effect on the biofilm formation in vitro. A standard laboratory strain, PAO1 was highly elongated during anaerobic respiration compared with bacteria grown aerobically. Microscopic analysis demonstrated that cell elongation likely occurred as a consequence of defective cell division. Cell elongation was dependent on the presence of nitrite reductase (NIR) that reduces nitrite (NO(2) (-)) to nitric oxide (NO) and was repressed in PAO1 in the presence of carboxy-PTIO, a NO antagonist, demonstrating that cell elongation involves a process to respond to NO, a spontaneous byproduct of the anaerobic respiration. Importantly, the non-elongated NIR-deficient mutant failed to form biofilm, while a mutant of nitrate reductase (NAR) and wild type PAO1, both of which were highly elongated, formed robust biofilm. Taken together, our data reveal a role of previously undescribed cell biological event in P. aeruginosa biofilm formation and suggest NIR as a key player involved in such process. |
| first_indexed | 2024-12-11T10:03:36Z |
| format | Article |
| id | doaj.art-978716d9b6e146e2be1a82e673b24bfc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T10:03:36Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-978716d9b6e146e2be1a82e673b24bfc2022-12-22T01:12:02ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1610510.1371/journal.pone.0016105Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration.Mi Young YoonKang-Mu LeeYongjin ParkSang Sun YoonPseudomonas aeruginosa, a gram-negative bacterium of clinical importance, forms more robust biofilm during anaerobic respiration, a mode of growth presumed to occur in abnormally thickened mucus layer lining the cystic fibrosis (CF) patient airway. However, molecular basis behind this anaerobiosis-triggered robust biofilm formation is not clearly defined yet. Here, we identified a morphological change naturally accompanied by anaerobic respiration in P. aeruginosa and investigated its effect on the biofilm formation in vitro. A standard laboratory strain, PAO1 was highly elongated during anaerobic respiration compared with bacteria grown aerobically. Microscopic analysis demonstrated that cell elongation likely occurred as a consequence of defective cell division. Cell elongation was dependent on the presence of nitrite reductase (NIR) that reduces nitrite (NO(2) (-)) to nitric oxide (NO) and was repressed in PAO1 in the presence of carboxy-PTIO, a NO antagonist, demonstrating that cell elongation involves a process to respond to NO, a spontaneous byproduct of the anaerobic respiration. Importantly, the non-elongated NIR-deficient mutant failed to form biofilm, while a mutant of nitrate reductase (NAR) and wild type PAO1, both of which were highly elongated, formed robust biofilm. Taken together, our data reveal a role of previously undescribed cell biological event in P. aeruginosa biofilm formation and suggest NIR as a key player involved in such process.http://europepmc.org/articles/PMC3022656?pdf=render |
| spellingShingle | Mi Young Yoon Kang-Mu Lee Yongjin Park Sang Sun Yoon Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration. PLoS ONE |
| title | Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration. |
| title_full | Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration. |
| title_fullStr | Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration. |
| title_full_unstemmed | Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration. |
| title_short | Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration. |
| title_sort | contribution of cell elongation to the biofilm formation of pseudomonas aeruginosa during anaerobic respiration |
| url | http://europepmc.org/articles/PMC3022656?pdf=render |
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