Entomotherapeutic Role of <i>Periplaneta americana</i> Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (<i>Locusta migratoria</i>) as an Ecotoxicological Model
In this study, we shed light for the first time on the usage of migratory locusts (<i>Locusta migratoria</i>) as an insect model to investigate the nanotoxicological influence of aluminum oxide (Al<sub>2</sub>O<sub>3</sub>) nanoparticles at low doses on testes, an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/3/653 |
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| author | Esraa A. Arafat Doaa S. El-Sayed Hussein K. Hussein Justin Flaven-Pouchon Bernard Moussian Lamia M. El-Samad Abeer El Wakil Mohamed A. Hassan |
| author_facet | Esraa A. Arafat Doaa S. El-Sayed Hussein K. Hussein Justin Flaven-Pouchon Bernard Moussian Lamia M. El-Samad Abeer El Wakil Mohamed A. Hassan |
| author_sort | Esraa A. Arafat |
| collection | DOAJ |
| description | In this study, we shed light for the first time on the usage of migratory locusts (<i>Locusta migratoria</i>) as an insect model to investigate the nanotoxicological influence of aluminum oxide (Al<sub>2</sub>O<sub>3</sub>) nanoparticles at low doses on testes, and evaluate the capacity of a whole-body extract of American cockroaches (<i>Periplaneta americana</i>) (PAE) to attenuate Al<sub>2</sub>O<sub>3</sub> NPs-induced toxicity. Energy dispersive X-ray microanalyzer (EDX) analysis verified the bioaccumulation of Al in testicular tissues due to its liberation from Al<sub>2</sub>O<sub>3</sub> NPs, implying their penetration into the blood–testis barrier. Remarkably, toxicity with Al engendered disorders of antioxidant and stress biomarkers associated with substantial DNA damage and cell apoptosis. Furthermore, histopathological and ultrastructural analyses manifested significant aberrations in the testicular tissues from the group exposed to Al<sub>2</sub>O<sub>3</sub> NPs, indicating the overproduction of reactive oxygen species (ROS). Molecular docking analysis emphasized the antioxidant capacity of some compounds derived from PAE. Thus, pretreatment with PAE counteracted the detrimental effects of Al in the testes, revealing antioxidant properties and thwarting DNA impairment and cell apoptosis. Moreover, histological and ultrastructural examinations revealed no anomalies in the testes. Overall, these findings substantiate the potential applications of PAE in preventing the testicular impairment of <i>L. migratoria</i> and the conceivable utilization of locusts for nanotoxicology studies. |
| first_indexed | 2024-03-11T07:00:45Z |
| format | Article |
| id | doaj.art-97875d98335b4ec1bf8c5084665e7898 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T07:00:45Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-97875d98335b4ec1bf8c5084665e78982023-11-17T09:17:52ZengMDPI AGAntioxidants2076-39212023-03-0112365310.3390/antiox12030653Entomotherapeutic Role of <i>Periplaneta americana</i> Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (<i>Locusta migratoria</i>) as an Ecotoxicological ModelEsraa A. Arafat0Doaa S. El-Sayed1Hussein K. Hussein2Justin Flaven-Pouchon3Bernard Moussian4Lamia M. El-Samad5Abeer El Wakil6Mohamed A. Hassan7Department of Zoology, Faculty of Science, Alexandria University, Alexandria 21321, EgyptChemistry Department, Faculty of Science, Alexandria University, Alexandria 21321, EgyptDepartment of Zoology, Faculty of Science, Alexandria University, Alexandria 21321, EgyptInterfaculty Institute for Cell Biology, Eberhard-Karls Universität Tübingen, 37073 Tübingen, GermanyUniversité Côte d’Azur, Parc Valrose, 06000 Nice, FranceDepartment of Zoology, Faculty of Science, Alexandria University, Alexandria 21321, EgyptDepartment of Biological and Geological Sciences, Faculty of Education, Alexandria University, Alexandria 21526, EgyptProtein Research Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technological Applications (SRTA-City), New Borg El-Arab City 21934, EgyptIn this study, we shed light for the first time on the usage of migratory locusts (<i>Locusta migratoria</i>) as an insect model to investigate the nanotoxicological influence of aluminum oxide (Al<sub>2</sub>O<sub>3</sub>) nanoparticles at low doses on testes, and evaluate the capacity of a whole-body extract of American cockroaches (<i>Periplaneta americana</i>) (PAE) to attenuate Al<sub>2</sub>O<sub>3</sub> NPs-induced toxicity. Energy dispersive X-ray microanalyzer (EDX) analysis verified the bioaccumulation of Al in testicular tissues due to its liberation from Al<sub>2</sub>O<sub>3</sub> NPs, implying their penetration into the blood–testis barrier. Remarkably, toxicity with Al engendered disorders of antioxidant and stress biomarkers associated with substantial DNA damage and cell apoptosis. Furthermore, histopathological and ultrastructural analyses manifested significant aberrations in the testicular tissues from the group exposed to Al<sub>2</sub>O<sub>3</sub> NPs, indicating the overproduction of reactive oxygen species (ROS). Molecular docking analysis emphasized the antioxidant capacity of some compounds derived from PAE. Thus, pretreatment with PAE counteracted the detrimental effects of Al in the testes, revealing antioxidant properties and thwarting DNA impairment and cell apoptosis. Moreover, histological and ultrastructural examinations revealed no anomalies in the testes. Overall, these findings substantiate the potential applications of PAE in preventing the testicular impairment of <i>L. migratoria</i> and the conceivable utilization of locusts for nanotoxicology studies.https://www.mdpi.com/2076-3921/12/3/653aluminum oxide NPs<i>Locusta migratoria</i>oxidative stresstesticular oxidative damages<i>Periplaneta americana</i> extract-derived insectsantioxidant activity |
| spellingShingle | Esraa A. Arafat Doaa S. El-Sayed Hussein K. Hussein Justin Flaven-Pouchon Bernard Moussian Lamia M. El-Samad Abeer El Wakil Mohamed A. Hassan Entomotherapeutic Role of <i>Periplaneta americana</i> Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (<i>Locusta migratoria</i>) as an Ecotoxicological Model Antioxidants aluminum oxide NPs <i>Locusta migratoria</i> oxidative stress testicular oxidative damages <i>Periplaneta americana</i> extract-derived insects antioxidant activity |
| title | Entomotherapeutic Role of <i>Periplaneta americana</i> Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (<i>Locusta migratoria</i>) as an Ecotoxicological Model |
| title_full | Entomotherapeutic Role of <i>Periplaneta americana</i> Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (<i>Locusta migratoria</i>) as an Ecotoxicological Model |
| title_fullStr | Entomotherapeutic Role of <i>Periplaneta americana</i> Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (<i>Locusta migratoria</i>) as an Ecotoxicological Model |
| title_full_unstemmed | Entomotherapeutic Role of <i>Periplaneta americana</i> Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (<i>Locusta migratoria</i>) as an Ecotoxicological Model |
| title_short | Entomotherapeutic Role of <i>Periplaneta americana</i> Extract in Alleviating Aluminum Oxide Nanoparticles-Induced Testicular Oxidative Impairment in Migratory Locusts (<i>Locusta migratoria</i>) as an Ecotoxicological Model |
| title_sort | entomotherapeutic role of i periplaneta americana i extract in alleviating aluminum oxide nanoparticles induced testicular oxidative impairment in migratory locusts i locusta migratoria i as an ecotoxicological model |
| topic | aluminum oxide NPs <i>Locusta migratoria</i> oxidative stress testicular oxidative damages <i>Periplaneta americana</i> extract-derived insects antioxidant activity |
| url | https://www.mdpi.com/2076-3921/12/3/653 |
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