The Enterotoxin Production and Antimicrobial Resistance of <i>Campylobacter</i> Strains Originating from Slaughter Animals
The pathogenicity of animal-origin <i>Campylobacter</i> strains, including antimicrobial resistance and enterotoxigenicity, was determined in this study. Overall, 149 <i>Campylobacter</i> isolates originating from cattle, swine and poultry were tested. The antimicrobial resis...
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MDPI AG
2022-09-01
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| Online Access: | https://www.mdpi.com/2076-0817/11/10/1131 |
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| author | Beata Wysok Joanna Wojtacka Agnieszka Wiszniewska-Łaszczych Marta Sołtysiuk Aleksandra Kobuszewska |
| author_facet | Beata Wysok Joanna Wojtacka Agnieszka Wiszniewska-Łaszczych Marta Sołtysiuk Aleksandra Kobuszewska |
| author_sort | Beata Wysok |
| collection | DOAJ |
| description | The pathogenicity of animal-origin <i>Campylobacter</i> strains, including antimicrobial resistance and enterotoxigenicity, was determined in this study. Overall, 149 <i>Campylobacter</i> isolates originating from cattle, swine and poultry were tested. The antimicrobial resistance profiles were examined by the diffusion disk method. The dominant resistance pattern was CIP_TET. The resistance rates for ciprofloxacin among swine, cattle and poultry isolates were 84%, 51% and 66%, respectively; for tetracycline, they were 82%, 57.1% and 76%, respectively. None of the obtained isolates was resistant to all four antimicrobials tested. The ability to produce enterotoxins was assessed by the use of a suckling mouse bioassay, with intestinal fluid accumulation as a positive result, and by CHO assay, with the elongation of cells as a positive result. The ability to produce enterotoxins was significantly higher among cattle isolates (61.2% and 71.4% positive isolates, respectively, in the bioassay and the CHO assay) than among swine (16% and 32% positive isolates, respectively) or poultry isolates (14% and 22% positive isolates, respectively). A strong positive correlation between in vitro and in vivo enterotoxicity tests was demonstrated. |
| first_indexed | 2024-03-09T19:37:43Z |
| format | Article |
| id | doaj.art-978bd5c2103040de8a81300598a51330 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T19:37:43Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj.art-978bd5c2103040de8a81300598a513302023-11-24T01:48:26ZengMDPI AGPathogens2076-08172022-09-011110113110.3390/pathogens11101131The Enterotoxin Production and Antimicrobial Resistance of <i>Campylobacter</i> Strains Originating from Slaughter AnimalsBeata Wysok0Joanna Wojtacka1Agnieszka Wiszniewska-Łaszczych2Marta Sołtysiuk3Aleksandra Kobuszewska4Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, PolandDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, PolandDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, PolandDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, PolandDepartment of Veterinary Public Health, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, PolandThe pathogenicity of animal-origin <i>Campylobacter</i> strains, including antimicrobial resistance and enterotoxigenicity, was determined in this study. Overall, 149 <i>Campylobacter</i> isolates originating from cattle, swine and poultry were tested. The antimicrobial resistance profiles were examined by the diffusion disk method. The dominant resistance pattern was CIP_TET. The resistance rates for ciprofloxacin among swine, cattle and poultry isolates were 84%, 51% and 66%, respectively; for tetracycline, they were 82%, 57.1% and 76%, respectively. None of the obtained isolates was resistant to all four antimicrobials tested. The ability to produce enterotoxins was assessed by the use of a suckling mouse bioassay, with intestinal fluid accumulation as a positive result, and by CHO assay, with the elongation of cells as a positive result. The ability to produce enterotoxins was significantly higher among cattle isolates (61.2% and 71.4% positive isolates, respectively, in the bioassay and the CHO assay) than among swine (16% and 32% positive isolates, respectively) or poultry isolates (14% and 22% positive isolates, respectively). A strong positive correlation between in vitro and in vivo enterotoxicity tests was demonstrated.https://www.mdpi.com/2076-0817/11/10/1131<i>Campylobacter</i>suckling mouse assayCHO assayenterotoxicityantimicrobial resistance |
| spellingShingle | Beata Wysok Joanna Wojtacka Agnieszka Wiszniewska-Łaszczych Marta Sołtysiuk Aleksandra Kobuszewska The Enterotoxin Production and Antimicrobial Resistance of <i>Campylobacter</i> Strains Originating from Slaughter Animals Pathogens <i>Campylobacter</i> suckling mouse assay CHO assay enterotoxicity antimicrobial resistance |
| title | The Enterotoxin Production and Antimicrobial Resistance of <i>Campylobacter</i> Strains Originating from Slaughter Animals |
| title_full | The Enterotoxin Production and Antimicrobial Resistance of <i>Campylobacter</i> Strains Originating from Slaughter Animals |
| title_fullStr | The Enterotoxin Production and Antimicrobial Resistance of <i>Campylobacter</i> Strains Originating from Slaughter Animals |
| title_full_unstemmed | The Enterotoxin Production and Antimicrobial Resistance of <i>Campylobacter</i> Strains Originating from Slaughter Animals |
| title_short | The Enterotoxin Production and Antimicrobial Resistance of <i>Campylobacter</i> Strains Originating from Slaughter Animals |
| title_sort | enterotoxin production and antimicrobial resistance of i campylobacter i strains originating from slaughter animals |
| topic | <i>Campylobacter</i> suckling mouse assay CHO assay enterotoxicity antimicrobial resistance |
| url | https://www.mdpi.com/2076-0817/11/10/1131 |
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