Aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetes
Sheri R Colberg1, Laura C Hill2, Henri K Parson3, Kathleen S Thomas1, Aaron I Vinik31Old Dominion University, Norfolk; 2State University of New York at Cortland, New York; 3Eastern Virginia Medical School, Norfolk, VirginiaAbstract: It is well known that a number of locally released vasodilatory and...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2010-08-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
| Online Access: | http://www.dovepress.com/aerobic-training-increases-skin-perfusion-by-a-nitric-oxide-mechanism--a4962 |
| _version_ | 1811173742077804544 |
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| author | Sheri R Colberg Laura C Hill Henri K Parson et al |
| author_facet | Sheri R Colberg Laura C Hill Henri K Parson et al |
| author_sort | Sheri R Colberg |
| collection | DOAJ |
| description | Sheri R Colberg1, Laura C Hill2, Henri K Parson3, Kathleen S Thomas1, Aaron I Vinik31Old Dominion University, Norfolk; 2State University of New York at Cortland, New York; 3Eastern Virginia Medical School, Norfolk, VirginiaAbstract: It is well known that a number of locally released vasodilatory and ­vasoconstrictive ­compounds can affect skin perfusion. This study investigated the effects of aerobic training on the contribution of nitric oxide (NO), prostaglandins (PG), and endothelial-derived ­hyperpolarizing factor (EDHF) in stimulated dorsal foot skin perfusion in individuals with type 2 diabetes (T2DM). Ten previously sedentary, older individuals with T2DM (57.0 ± 3.1 years) and nine sedentary controls (53.5 ± 3.2 years) were tested before and after undertaking six months of moderate a­erobic training three times weekly in a supervised setting. All subjects underwent measurement of ­baseline (32°C) and heat-stimulated (40°C and 44°C) dorsal foot skin perfusion starting one hour after ­ingestion of a single, oral 325 mg dose of aspirin, a known inhibitor of PG synthesis. Before aspirin ­ingestion, a subcutaneous microdialysis probe was inserted into each foot dorsum to administer either saline (PG pathway only blocked by aspirin in the left foot) or L-NAME (N(G)-nitro-l-arginine methyl ester; thereby inhibiting both PG and NO pathways in the right foot). Normative data collected previously on subjects undergoing saline administration via ­microdialysis without aspirin ingestion served as a control group. Significantly lower responsiveness of maximal perfusion was found with the EDHF pathway alone unblocked compared with NO and EDHF unblocked after training. Maximal suppression attributable directly to NO, PG, and EDHF was not significantly different when examined by subject group and training status. However, ­contributions of NO, PG, and EDHF to maximal perfusion were significantly increased, decreased, and unchanged by aerobic training, respectively, with diabetic and control subjects combined due to nonsignificant differences between groups. Improvements in maximally stimulated dorsal foot skin perfusion resulting from six months of aerobic training appear to have primarily an NO basis, with lesser contributions from PG following training, regardless of diabetes status.Keywords: skin perfusion, aerobic training, type 2 diabetes, nitric oxide, prostaglandins, endothelial-derived hyperpolarizing factor, microdialysis |
| first_indexed | 2024-04-10T17:52:06Z |
| format | Article |
| id | doaj.art-978ce4d8b3574aa081c690f503c60fea |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-10T17:52:06Z |
| publishDate | 2010-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-978ce4d8b3574aa081c690f503c60fea2023-02-02T20:27:05ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072010-08-012010default275280Aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetesSheri R ColbergLaura C HillHenri K Parsonet alSheri R Colberg1, Laura C Hill2, Henri K Parson3, Kathleen S Thomas1, Aaron I Vinik31Old Dominion University, Norfolk; 2State University of New York at Cortland, New York; 3Eastern Virginia Medical School, Norfolk, VirginiaAbstract: It is well known that a number of locally released vasodilatory and ­vasoconstrictive ­compounds can affect skin perfusion. This study investigated the effects of aerobic training on the contribution of nitric oxide (NO), prostaglandins (PG), and endothelial-derived ­hyperpolarizing factor (EDHF) in stimulated dorsal foot skin perfusion in individuals with type 2 diabetes (T2DM). Ten previously sedentary, older individuals with T2DM (57.0 ± 3.1 years) and nine sedentary controls (53.5 ± 3.2 years) were tested before and after undertaking six months of moderate a­erobic training three times weekly in a supervised setting. All subjects underwent measurement of ­baseline (32°C) and heat-stimulated (40°C and 44°C) dorsal foot skin perfusion starting one hour after ­ingestion of a single, oral 325 mg dose of aspirin, a known inhibitor of PG synthesis. Before aspirin ­ingestion, a subcutaneous microdialysis probe was inserted into each foot dorsum to administer either saline (PG pathway only blocked by aspirin in the left foot) or L-NAME (N(G)-nitro-l-arginine methyl ester; thereby inhibiting both PG and NO pathways in the right foot). Normative data collected previously on subjects undergoing saline administration via ­microdialysis without aspirin ingestion served as a control group. Significantly lower responsiveness of maximal perfusion was found with the EDHF pathway alone unblocked compared with NO and EDHF unblocked after training. Maximal suppression attributable directly to NO, PG, and EDHF was not significantly different when examined by subject group and training status. However, ­contributions of NO, PG, and EDHF to maximal perfusion were significantly increased, decreased, and unchanged by aerobic training, respectively, with diabetic and control subjects combined due to nonsignificant differences between groups. Improvements in maximally stimulated dorsal foot skin perfusion resulting from six months of aerobic training appear to have primarily an NO basis, with lesser contributions from PG following training, regardless of diabetes status.Keywords: skin perfusion, aerobic training, type 2 diabetes, nitric oxide, prostaglandins, endothelial-derived hyperpolarizing factor, microdialysishttp://www.dovepress.com/aerobic-training-increases-skin-perfusion-by-a-nitric-oxide-mechanism--a4962 |
| spellingShingle | Sheri R Colberg Laura C Hill Henri K Parson et al Aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetes Diabetes, Metabolic Syndrome and Obesity |
| title | Aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetes |
| title_full | Aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetes |
| title_fullStr | Aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetes |
| title_full_unstemmed | Aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetes |
| title_short | Aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetes |
| title_sort | aerobic training increases skin perfusion by a nitric oxide mechanism in type 2 diabetes |
| url | http://www.dovepress.com/aerobic-training-increases-skin-perfusion-by-a-nitric-oxide-mechanism--a4962 |
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