Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis
Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evi...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-09-01
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| Series: | Frontiers in Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmed.2019.00203/full |
| _version_ | 1811274647175430144 |
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| author | Manon Kolb Vladimir Lazarevic Stéphane Emonet Stéphane Emonet Alexandra Calmy Myriam Girard Nadia Gaïa Yannick Charretier Abdessalam Cherkaoui Peter Keller Christoph Huber Jacques Schrenzel Jacques Schrenzel Jacques Schrenzel |
| author_facet | Manon Kolb Vladimir Lazarevic Stéphane Emonet Stéphane Emonet Alexandra Calmy Myriam Girard Nadia Gaïa Yannick Charretier Abdessalam Cherkaoui Peter Keller Christoph Huber Jacques Schrenzel Jacques Schrenzel Jacques Schrenzel |
| author_sort | Manon Kolb |
| collection | DOAJ |
| description | Diagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis. |
| first_indexed | 2024-04-12T23:22:51Z |
| format | Article |
| id | doaj.art-978e74bc058b46a196cf5b231f29cc3d |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-12T23:22:51Z |
| publishDate | 2019-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-978e74bc058b46a196cf5b231f29cc3d2022-12-22T03:12:29ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2019-09-01610.3389/fmed.2019.00203474365Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative EndocarditisManon Kolb0Vladimir Lazarevic1Stéphane Emonet2Stéphane Emonet3Alexandra Calmy4Myriam Girard5Nadia Gaïa6Yannick Charretier7Abdessalam Cherkaoui8Peter Keller9Christoph Huber10Jacques Schrenzel11Jacques Schrenzel12Jacques Schrenzel13Service of General Internal Medicine, Geneva University Hospitals (HUG), Geneva, SwitzerlandGenomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, SwitzerlandBacteriology Laboratory, Service of Laboratory Medicine, Geneva University Hospitals, Geneva, SwitzerlandService of Infectious Diseases, Geneva University Hospitals, Geneva, SwitzerlandService of Infectious Diseases, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, SwitzerlandBacteriology Laboratory, Service of Laboratory Medicine, Geneva University Hospitals, Geneva, SwitzerlandInstitute of Medical Microbiology, University of Zurich, Zurich, SwitzerlandService of Cardiovascular Surgery, Geneva University Hospitals, Geneva, SwitzerlandGenomic Research Laboratory, Service of Infectious Diseases, Geneva University Hospitals, Geneva, SwitzerlandBacteriology Laboratory, Service of Laboratory Medicine, Geneva University Hospitals, Geneva, SwitzerlandService of Infectious Diseases, Geneva University Hospitals, Geneva, SwitzerlandDiagnosis of culture-negative infective endocarditis usually implies indirect pathogen identification by serologic or molecular techniques. Clinical metagenomics, relying on next-generation sequencing (NGS) is an emerging approach that allows pathogen identification in challenging situations, as evidenced by a clinical case. We sequenced the DNA extracted from the surgically-removed frozen valve tissue from a patient with suspected infective endocarditis with negative blood and valve cultures. Mapping of the sequence reads against reference genomic sequences, a 16S rRNA gene database and clade-specific marker genes suggested an infection caused by Cardiobacterium hominis.https://www.frontiersin.org/article/10.3389/fmed.2019.00203/fullnext-generation sequencingculture-negative endocarditisclinical metagenomicsCardiobacterium hominisdiagnosis |
| spellingShingle | Manon Kolb Vladimir Lazarevic Stéphane Emonet Stéphane Emonet Alexandra Calmy Myriam Girard Nadia Gaïa Yannick Charretier Abdessalam Cherkaoui Peter Keller Christoph Huber Jacques Schrenzel Jacques Schrenzel Jacques Schrenzel Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis Frontiers in Medicine next-generation sequencing culture-negative endocarditis clinical metagenomics Cardiobacterium hominis diagnosis |
| title | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
| title_full | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
| title_fullStr | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
| title_full_unstemmed | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
| title_short | Next-Generation Sequencing for the Diagnosis of Challenging Culture-Negative Endocarditis |
| title_sort | next generation sequencing for the diagnosis of challenging culture negative endocarditis |
| topic | next-generation sequencing culture-negative endocarditis clinical metagenomics Cardiobacterium hominis diagnosis |
| url | https://www.frontiersin.org/article/10.3389/fmed.2019.00203/full |
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