Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism
IntroductionEndophytes play a significant role in regulating plant root development and facilitating nutrient solubilization and transportation. This association could improve plant growth. The present study has uncovered a distinct phenotype, which we refer to as "white root", arising fro...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1326917/full |
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| author | Mingzheng Duan Mingzheng Duan Xiang Li Xiaojian Wu Shengfeng Long Hairong Huang Yijie Li Qi-Huai Liu Guanghu Zhu Bin Feng Sunqian Qin Changning Li Hai Yang Jie Qin Zhendong Chen Zeping Wang |
| author_facet | Mingzheng Duan Mingzheng Duan Xiang Li Xiaojian Wu Shengfeng Long Hairong Huang Yijie Li Qi-Huai Liu Guanghu Zhu Bin Feng Sunqian Qin Changning Li Hai Yang Jie Qin Zhendong Chen Zeping Wang |
| author_sort | Mingzheng Duan |
| collection | DOAJ |
| description | IntroductionEndophytes play a significant role in regulating plant root development and facilitating nutrient solubilization and transportation. This association could improve plant growth. The present study has uncovered a distinct phenotype, which we refer to as "white root", arising from the intricate interactions between endophytic fungi and bacteria with the roots in a sugarcane and bamboo fungus (Dictyophora indusiata) intercropping system.MethodsWe investigated the mechanisms underlying the formation of this “white root” phenotype and its impact on sugarcane yield and metabolism by metabarcoding and metabolome analysis.Results and DiscussionInitial analysis revealed that intercropping with D. indusiata increased sugarcane yield by enhancing the number of viable tillers compared with bagasse and no input control. Metabarcoding based on second-generation and third-generation sequencing indicated that D. indusiate and Bacillus aryabhattai dominates the fungal and bacterial composition in the “white root” phenotype of sugarcane root. The coexistence of D. indusiata and B. aryabhattai as endophytes induced plant growth-promoting metabolites in the sugarcane root system, such as lysoPC 18:1 and dihydrobenzofuran, probably contributing to increased sugarcane yield. Furthermore, the association also enhanced the metabolism of compounds, such as naringenin-7-O-glucoside (Prunin), naringenin-7-O-neohesperidoside (Naringin)*, hesperetin-7-O-neohesperidoside (Neohesperidin), epicatechin, and aromadendrin (Dihydrokaempferol), involved in flavonoid metabolism during the formation of the endophytic phenotype in the sugarcane root system. These observations suggest that the “white root” phenotype promotes sugarcane growth by activating flavonoid metabolism. This study reports an interesting phenomenon where D. indusiata, coordinate with the specific bacteria invade, forms a “white root” phenotype with sugarcane root. The study also provides new insights into using D. indusiata as a soil inoculant for promoting sugarcane growth and proposes a new approach for improve sugarcane cultivation. |
| first_indexed | 2024-03-07T14:02:15Z |
| format | Article |
| id | doaj.art-9791ed46afed40e5acac043a6269058d |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-07T14:02:15Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9791ed46afed40e5acac043a6269058d2024-03-07T05:03:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-03-011510.3389/fpls.2024.13269171326917Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolismMingzheng Duan0Mingzheng Duan1Xiang Li2Xiaojian Wu3Shengfeng Long4Hairong Huang5Yijie Li6Qi-Huai Liu7Guanghu Zhu8Bin Feng9Sunqian Qin10Changning Li11Hai Yang12Jie Qin13Zhendong Chen14Zeping Wang15Guangxi Academy of Agricultural Sciences, Nanning, ChinaYunnan Key Laboratory of Gastrodia Elata and Fungal Symbiotic Biology, College of Agronomy and Life Sciences, Zhaotong University, Zhaotong, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaLaibin Academy of Agricultural Sciences, Laibin, ChinaLaibin Academy of Agricultural Sciences, Laibin, ChinaLaibin Academy of Agricultural Sciences, Laibin, ChinaLaibin Academy of Agricultural Sciences, Laibin, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaGuangxi Academy of Agricultural Sciences, Nanning, ChinaIntroductionEndophytes play a significant role in regulating plant root development and facilitating nutrient solubilization and transportation. This association could improve plant growth. The present study has uncovered a distinct phenotype, which we refer to as "white root", arising from the intricate interactions between endophytic fungi and bacteria with the roots in a sugarcane and bamboo fungus (Dictyophora indusiata) intercropping system.MethodsWe investigated the mechanisms underlying the formation of this “white root” phenotype and its impact on sugarcane yield and metabolism by metabarcoding and metabolome analysis.Results and DiscussionInitial analysis revealed that intercropping with D. indusiata increased sugarcane yield by enhancing the number of viable tillers compared with bagasse and no input control. Metabarcoding based on second-generation and third-generation sequencing indicated that D. indusiate and Bacillus aryabhattai dominates the fungal and bacterial composition in the “white root” phenotype of sugarcane root. The coexistence of D. indusiata and B. aryabhattai as endophytes induced plant growth-promoting metabolites in the sugarcane root system, such as lysoPC 18:1 and dihydrobenzofuran, probably contributing to increased sugarcane yield. Furthermore, the association also enhanced the metabolism of compounds, such as naringenin-7-O-glucoside (Prunin), naringenin-7-O-neohesperidoside (Naringin)*, hesperetin-7-O-neohesperidoside (Neohesperidin), epicatechin, and aromadendrin (Dihydrokaempferol), involved in flavonoid metabolism during the formation of the endophytic phenotype in the sugarcane root system. These observations suggest that the “white root” phenotype promotes sugarcane growth by activating flavonoid metabolism. This study reports an interesting phenomenon where D. indusiata, coordinate with the specific bacteria invade, forms a “white root” phenotype with sugarcane root. The study also provides new insights into using D. indusiata as a soil inoculant for promoting sugarcane growth and proposes a new approach for improve sugarcane cultivation.https://www.frontiersin.org/articles/10.3389/fpls.2024.1326917/fullBacillusfull-length 16S rRNAmetabolomemetabarcodingplant growthsugarcane root |
| spellingShingle | Mingzheng Duan Mingzheng Duan Xiang Li Xiaojian Wu Shengfeng Long Hairong Huang Yijie Li Qi-Huai Liu Guanghu Zhu Bin Feng Sunqian Qin Changning Li Hai Yang Jie Qin Zhendong Chen Zeping Wang Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism Frontiers in Plant Science Bacillus full-length 16S rRNA metabolome metabarcoding plant growth sugarcane root |
| title | Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism |
| title_full | Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism |
| title_fullStr | Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism |
| title_full_unstemmed | Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism |
| title_short | Dictyophora indusiata and Bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism |
| title_sort | dictyophora indusiata and bacillus aryabhattai improve sugarcane yield by endogenously associating with the root and regulating flavonoid metabolism |
| topic | Bacillus full-length 16S rRNA metabolome metabarcoding plant growth sugarcane root |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1326917/full |
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