Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic Communities
With a growing world population and increasing frequency of climate disturbance events, we are in dire need of methods to improve plant productivity, resilience, and resistance to both abiotic and biotic stressors, both for agriculture and conservation efforts. Microorganisms play an essential role...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-06-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.910377/full |
| _version_ | 1828725765485625344 |
|---|---|
| author | Ashley E. Beck Manuel Kleiner Anna-Katharina Garrell |
| author_facet | Ashley E. Beck Manuel Kleiner Anna-Katharina Garrell |
| author_sort | Ashley E. Beck |
| collection | DOAJ |
| description | With a growing world population and increasing frequency of climate disturbance events, we are in dire need of methods to improve plant productivity, resilience, and resistance to both abiotic and biotic stressors, both for agriculture and conservation efforts. Microorganisms play an essential role in supporting plant growth, environmental response, and susceptibility to disease. However, understanding the specific mechanisms by which microbes interact with each other and with plants to influence plant phenotypes is a major challenge due to the complexity of natural communities, simultaneous competition and cooperation effects, signalling interactions, and environmental impacts. Synthetic communities are a major asset in reducing the complexity of these systems by simplifying to dominant components and isolating specific variables for controlled experiments, yet there still remains a large gap in our understanding of plant microbiome interactions. This perspectives article presents a brief review discussing ways in which metabolic modelling can be used in combination with synthetic communities to continue progress toward understanding the complexity of plant-microbe-environment interactions. We highlight the utility of metabolic models as applied to a community setting, identify different applications for both flux balance and elementary flux mode simulation approaches, emphasize the importance of ecological theory in guiding data interpretation, and provide ideas for how the integration of metabolic modelling techniques with big data may bridge the gap between simplified synthetic communities and the complexity of natural plant-microbe systems. |
| first_indexed | 2024-04-12T13:32:11Z |
| format | Article |
| id | doaj.art-9793fbce454442d39a9b3cfca88a2827 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T13:32:11Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-9793fbce454442d39a9b3cfca88a28272022-12-22T03:31:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.910377910377Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic CommunitiesAshley E. Beck0Manuel Kleiner1Anna-Katharina Garrell2Department of Biological and Environmental Sciences, Carroll College, Helena, MT, United StatesDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United StatesDepartment of Plant and Microbial Biology, North Carolina State University, Raleigh, NC, United StatesWith a growing world population and increasing frequency of climate disturbance events, we are in dire need of methods to improve plant productivity, resilience, and resistance to both abiotic and biotic stressors, both for agriculture and conservation efforts. Microorganisms play an essential role in supporting plant growth, environmental response, and susceptibility to disease. However, understanding the specific mechanisms by which microbes interact with each other and with plants to influence plant phenotypes is a major challenge due to the complexity of natural communities, simultaneous competition and cooperation effects, signalling interactions, and environmental impacts. Synthetic communities are a major asset in reducing the complexity of these systems by simplifying to dominant components and isolating specific variables for controlled experiments, yet there still remains a large gap in our understanding of plant microbiome interactions. This perspectives article presents a brief review discussing ways in which metabolic modelling can be used in combination with synthetic communities to continue progress toward understanding the complexity of plant-microbe-environment interactions. We highlight the utility of metabolic models as applied to a community setting, identify different applications for both flux balance and elementary flux mode simulation approaches, emphasize the importance of ecological theory in guiding data interpretation, and provide ideas for how the integration of metabolic modelling techniques with big data may bridge the gap between simplified synthetic communities and the complexity of natural plant-microbe systems.https://www.frontiersin.org/articles/10.3389/fpls.2022.910377/fullsynthetic communitiesplant microbiomeplant microbial interactionsmetabolic modellingflux balance analysiselementary flux mode analysis |
| spellingShingle | Ashley E. Beck Manuel Kleiner Anna-Katharina Garrell Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic Communities Frontiers in Plant Science synthetic communities plant microbiome plant microbial interactions metabolic modelling flux balance analysis elementary flux mode analysis |
| title | Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic Communities |
| title_full | Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic Communities |
| title_fullStr | Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic Communities |
| title_full_unstemmed | Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic Communities |
| title_short | Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic Communities |
| title_sort | elucidating plant microbe environment interactions through omics enabled metabolic modelling using synthetic communities |
| topic | synthetic communities plant microbiome plant microbial interactions metabolic modelling flux balance analysis elementary flux mode analysis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.910377/full |
| work_keys_str_mv | AT ashleyebeck elucidatingplantmicrobeenvironmentinteractionsthroughomicsenabledmetabolicmodellingusingsyntheticcommunities AT manuelkleiner elucidatingplantmicrobeenvironmentinteractionsthroughomicsenabledmetabolicmodellingusingsyntheticcommunities AT annakatharinagarrell elucidatingplantmicrobeenvironmentinteractionsthroughomicsenabledmetabolicmodellingusingsyntheticcommunities |