Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by <i>Bacillus cereus</i> on Stainless Steel Surfaces in Food Processing Facilities
The <i>Bacillus cereus</i> (<i>B. cereus</i>) group is a widespread foodborne pathogen with a persistent ability to form biofilm, and with inherent resistance to traditional treatment in the food industry. Bacteriophages are a promising biocontrol agent that could be applied...
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MDPI AG
2022-08-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/8/872 |
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| author | Maroua Gdoura-Ben Amor Antoine Culot Clarisse Techer Mousa AlReshidi Mohd Adnan Sophie Jan Florence Baron Noël Grosset Mejdi Snoussi Radhouane Gdoura Michel Gautier |
| author_facet | Maroua Gdoura-Ben Amor Antoine Culot Clarisse Techer Mousa AlReshidi Mohd Adnan Sophie Jan Florence Baron Noël Grosset Mejdi Snoussi Radhouane Gdoura Michel Gautier |
| author_sort | Maroua Gdoura-Ben Amor |
| collection | DOAJ |
| description | The <i>Bacillus cereus</i> (<i>B. cereus</i>) group is a widespread foodborne pathogen with a persistent ability to form biofilm, and with inherent resistance to traditional treatment in the food industry. Bacteriophages are a promising biocontrol agent that could be applied to prevent or eliminate biofilms formation. We have described, in this study, the isolation from sewage samples and preliminary characterization of bacteriophages that are active against the <i>B. cereus</i> group. The effectiveness of phage treatment for reducing <i>B. cereus</i> attachment and biofilms on stainless steel surfaces has been also assessed using three incubation periods at different titrations of each phage. Out of 62 phages isolated, seven showed broad-spectrum lytic action against 174 <i>B. cereus</i> isolates. All selected phages appeared to be of the <i>Siphoviridae</i> family. SDS-PAGE proved that two phages have a similar profile, while the remainder are distinct. All isolated phages have the same restriction pattern, with an estimated genome size of around 37 kb. The isolated bacteriophages have been shown to be effective in preventing biofilm formation. Reductions of up to 1.5 log<sub>10</sub> UFC/cm<sup>2</sup> have been achieved, compared to the untreated biofilms. Curative treatment reduced the bacterial density by 0.5 log<sub>10</sub> UFC/cm<sup>2</sup>. These results support the prospect of using these phages as a potential alternative strategy for controlling biofilms in food systems. |
| first_indexed | 2024-03-09T12:49:11Z |
| format | Article |
| id | doaj.art-979422a1a4974bc290df4d893d320d8d |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T12:49:11Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-979422a1a4974bc290df4d893d320d8d2023-11-30T22:09:18ZengMDPI AGPathogens2076-08172022-08-0111887210.3390/pathogens11080872Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by <i>Bacillus cereus</i> on Stainless Steel Surfaces in Food Processing FacilitiesMaroua Gdoura-Ben Amor0Antoine Culot1Clarisse Techer2Mousa AlReshidi3Mohd Adnan4Sophie Jan5Florence Baron6Noël Grosset7Mejdi Snoussi8Radhouane Gdoura9Michel Gautier10Laboratory Research of Toxicology-Microbiology Environmental and Health LR17ES06, Sciences Faculty of Sfax, University of Sfax, Sfax 3000, TunisiaEquipe Microbiologie, Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l’Œuf, 35042 Rennes, FranceMixscience, Rue des Courtillons, ZAC Cissé Blossac, 35712 Bruz, FranceDepartment of Biology, College of Science, University of Hail, Ha’il P.O. Box 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Hail, Ha’il P.O. Box 2440, Saudi ArabiaEquipe Microbiologie, Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l’Œuf, 35042 Rennes, FranceEquipe Microbiologie, Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l’Œuf, 35042 Rennes, FranceEquipe Microbiologie, Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l’Œuf, 35042 Rennes, FranceDepartment of Biology, College of Science, University of Hail, Ha’il P.O. Box 2440, Saudi ArabiaLaboratory Research of Toxicology-Microbiology Environmental and Health LR17ES06, Sciences Faculty of Sfax, University of Sfax, Sfax 3000, TunisiaEquipe Microbiologie, Agrocampus Ouest, INRA, UMR1253 Science et Technologie du Lait et de l’Œuf, 35042 Rennes, FranceThe <i>Bacillus cereus</i> (<i>B. cereus</i>) group is a widespread foodborne pathogen with a persistent ability to form biofilm, and with inherent resistance to traditional treatment in the food industry. Bacteriophages are a promising biocontrol agent that could be applied to prevent or eliminate biofilms formation. We have described, in this study, the isolation from sewage samples and preliminary characterization of bacteriophages that are active against the <i>B. cereus</i> group. The effectiveness of phage treatment for reducing <i>B. cereus</i> attachment and biofilms on stainless steel surfaces has been also assessed using three incubation periods at different titrations of each phage. Out of 62 phages isolated, seven showed broad-spectrum lytic action against 174 <i>B. cereus</i> isolates. All selected phages appeared to be of the <i>Siphoviridae</i> family. SDS-PAGE proved that two phages have a similar profile, while the remainder are distinct. All isolated phages have the same restriction pattern, with an estimated genome size of around 37 kb. The isolated bacteriophages have been shown to be effective in preventing biofilm formation. Reductions of up to 1.5 log<sub>10</sub> UFC/cm<sup>2</sup> have been achieved, compared to the untreated biofilms. Curative treatment reduced the bacterial density by 0.5 log<sub>10</sub> UFC/cm<sup>2</sup>. These results support the prospect of using these phages as a potential alternative strategy for controlling biofilms in food systems.https://www.mdpi.com/2076-0817/11/8/872<i>B. cereus</i> groupbiofilmstainless steelbacteriophagesewagecharacterization |
| spellingShingle | Maroua Gdoura-Ben Amor Antoine Culot Clarisse Techer Mousa AlReshidi Mohd Adnan Sophie Jan Florence Baron Noël Grosset Mejdi Snoussi Radhouane Gdoura Michel Gautier Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by <i>Bacillus cereus</i> on Stainless Steel Surfaces in Food Processing Facilities Pathogens <i>B. cereus</i> group biofilm stainless steel bacteriophage sewage characterization |
| title | Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by <i>Bacillus cereus</i> on Stainless Steel Surfaces in Food Processing Facilities |
| title_full | Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by <i>Bacillus cereus</i> on Stainless Steel Surfaces in Food Processing Facilities |
| title_fullStr | Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by <i>Bacillus cereus</i> on Stainless Steel Surfaces in Food Processing Facilities |
| title_full_unstemmed | Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by <i>Bacillus cereus</i> on Stainless Steel Surfaces in Food Processing Facilities |
| title_short | Isolation, Partial Characterization and Application of Bacteriophages in Eradicating Biofilm Formation by <i>Bacillus cereus</i> on Stainless Steel Surfaces in Food Processing Facilities |
| title_sort | isolation partial characterization and application of bacteriophages in eradicating biofilm formation by i bacillus cereus i on stainless steel surfaces in food processing facilities |
| topic | <i>B. cereus</i> group biofilm stainless steel bacteriophage sewage characterization |
| url | https://www.mdpi.com/2076-0817/11/8/872 |
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