Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean
Abstract Background Soybean developing seed is susceptible to high temperature and humidity (HTH) stress in the field, resulting in vigor reduction. Actually, the HTH in the field during soybean seed growth and development would also stress the whole plant, especially on leaf and pod, which in turn...
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| Format: | Article |
| Language: | English |
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BMC
2020-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02335-1 |
| _version_ | 1828751035101872128 |
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| author | Jiaping Wei Xiaolin Liu Linzhi Li Haihong Zhao Sushuang Liu Xingwang Yu Yingzi Shen Yali Zhou Yajing Zhu Yingjie Shu Hao Ma |
| author_facet | Jiaping Wei Xiaolin Liu Linzhi Li Haihong Zhao Sushuang Liu Xingwang Yu Yingzi Shen Yali Zhou Yajing Zhu Yingjie Shu Hao Ma |
| author_sort | Jiaping Wei |
| collection | DOAJ |
| description | Abstract Background Soybean developing seed is susceptible to high temperature and humidity (HTH) stress in the field, resulting in vigor reduction. Actually, the HTH in the field during soybean seed growth and development would also stress the whole plant, especially on leaf and pod, which in turn affect seed growth and development as well as vigor formation through nutrient supply and protection. Results In the present study, using a pair of pre-harvest seed deterioration-sensitive and -resistant cultivars Ningzhen No. 1 and Xiangdou No. 3, the comprehensive effects of HTH stress on seed vigor formation during physiological maturity were investigated by analyzing cotyledon, embryo, leaf, and pod at the levels of protein, ultrastructure, and physiology and biochemistry. There were 247, 179, and 517 differentially abundant proteins (DAPs) identified in cotyledon, embryo, and leaf of cv. Xiangdou No. 3 under HTH stress, while 235, 366, and 479 DAPs were identified in cotyledon, embryo, and leaf of cv. Ningzhen No. 1. Moreover, 120, 144, and 438 DAPs between the two cultivars were identified in cotyledon, embryo, and leaf under HTH stress, respectively. Moreover, 120, 144, and 438 DAPs between the two cultivars were identified in cotyledon, embryo, and leaf under HTH stress, respectively. Most of the DAPs identified were found to be involved in major metabolic pathways and cellular processes, including signal transduction, tricarboxylic acid cycle, fatty acid metabolism, photosynthesis, protein processing, folding and assembly, protein biosynthesis or degradation, plant-pathogen interaction, starch and sucrose metabolism, and oxidative stress response. The HTH stress had less negative effects on metabolic pathways, cell ultrastructure, and physiology and biochemistry in the four organs of Xiangdou No. 3 than in those of Ningzhen No. 1, leading to produce higher vigor seeds in the former. Conclusion High seed vigor formation is enhanced by increasing protein biosynthesis and nutrient storage in cotyledon, stronger stability and viability in embryo, more powerful photosynthetic capacity and nutrient supply in leaf, and stronger protection in pod under HTH stress. These results provide comprehensive characteristics of leaf, pod and seed (cotyledon and embryo) under HTH stress, and some of them can be used as selection index in high seed vigor breeding program in soybean. |
| first_indexed | 2024-12-10T20:47:21Z |
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| id | doaj.art-97993f88056d419a9cf42a58a4a5b873 |
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| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-10T20:47:21Z |
| publishDate | 2020-03-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-97993f88056d419a9cf42a58a4a5b8732022-12-22T01:34:12ZengBMCBMC Plant Biology1471-22292020-03-0120111510.1186/s12870-020-02335-1Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybeanJiaping Wei0Xiaolin Liu1Linzhi Li2Haihong Zhao3Sushuang Liu4Xingwang Yu5Yingzi Shen6Yali Zhou7Yajing Zhu8Yingjie Shu9Hao Ma10State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural UniversityAbstract Background Soybean developing seed is susceptible to high temperature and humidity (HTH) stress in the field, resulting in vigor reduction. Actually, the HTH in the field during soybean seed growth and development would also stress the whole plant, especially on leaf and pod, which in turn affect seed growth and development as well as vigor formation through nutrient supply and protection. Results In the present study, using a pair of pre-harvest seed deterioration-sensitive and -resistant cultivars Ningzhen No. 1 and Xiangdou No. 3, the comprehensive effects of HTH stress on seed vigor formation during physiological maturity were investigated by analyzing cotyledon, embryo, leaf, and pod at the levels of protein, ultrastructure, and physiology and biochemistry. There were 247, 179, and 517 differentially abundant proteins (DAPs) identified in cotyledon, embryo, and leaf of cv. Xiangdou No. 3 under HTH stress, while 235, 366, and 479 DAPs were identified in cotyledon, embryo, and leaf of cv. Ningzhen No. 1. Moreover, 120, 144, and 438 DAPs between the two cultivars were identified in cotyledon, embryo, and leaf under HTH stress, respectively. Moreover, 120, 144, and 438 DAPs between the two cultivars were identified in cotyledon, embryo, and leaf under HTH stress, respectively. Most of the DAPs identified were found to be involved in major metabolic pathways and cellular processes, including signal transduction, tricarboxylic acid cycle, fatty acid metabolism, photosynthesis, protein processing, folding and assembly, protein biosynthesis or degradation, plant-pathogen interaction, starch and sucrose metabolism, and oxidative stress response. The HTH stress had less negative effects on metabolic pathways, cell ultrastructure, and physiology and biochemistry in the four organs of Xiangdou No. 3 than in those of Ningzhen No. 1, leading to produce higher vigor seeds in the former. Conclusion High seed vigor formation is enhanced by increasing protein biosynthesis and nutrient storage in cotyledon, stronger stability and viability in embryo, more powerful photosynthetic capacity and nutrient supply in leaf, and stronger protection in pod under HTH stress. These results provide comprehensive characteristics of leaf, pod and seed (cotyledon and embryo) under HTH stress, and some of them can be used as selection index in high seed vigor breeding program in soybean.http://link.springer.com/article/10.1186/s12870-020-02335-1SoybeanHigh temperature and humidity stressSeed vigorProteomicUltrastructurePhysiology and biochemistry |
| spellingShingle | Jiaping Wei Xiaolin Liu Linzhi Li Haihong Zhao Sushuang Liu Xingwang Yu Yingzi Shen Yali Zhou Yajing Zhu Yingjie Shu Hao Ma Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean BMC Plant Biology Soybean High temperature and humidity stress Seed vigor Proteomic Ultrastructure Physiology and biochemistry |
| title | Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean |
| title_full | Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean |
| title_fullStr | Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean |
| title_full_unstemmed | Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean |
| title_short | Quantitative proteomic, physiological and biochemical analysis of cotyledon, embryo, leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean |
| title_sort | quantitative proteomic physiological and biochemical analysis of cotyledon embryo leaf and pod reveals the effects of high temperature and humidity stress on seed vigor formation in soybean |
| topic | Soybean High temperature and humidity stress Seed vigor Proteomic Ultrastructure Physiology and biochemistry |
| url | http://link.springer.com/article/10.1186/s12870-020-02335-1 |
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