The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium
In this study, the influence of cultivation time on xanthan biosynthesis by different Xanthomonas strains, reference strain and crucifers’ and pepper leaves’ isolates, was examined. Biopolymer was produced by submerged cultivation of fourteen producing strains on medium with glycerol as a s...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Faculty of Technology, Novi Sad
2021-01-01
|
| Series: | Acta Periodica Technologica |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-7188/2021/1450-71882152173Z.pdf |
| _version_ | 1823968183048994816 |
|---|---|
| author | Zahović Ida E. Dodić Jelena M. Grahovac Jovana A. Grahovac Mila S. Trivunović Zorana Z. |
| author_facet | Zahović Ida E. Dodić Jelena M. Grahovac Jovana A. Grahovac Mila S. Trivunović Zorana Z. |
| author_sort | Zahović Ida E. |
| collection | DOAJ |
| description | In this study, the influence of cultivation time on xanthan biosynthesis by
different Xanthomonas strains, reference strain and crucifers’ and pepper
leaves’ isolates, was examined. Biopolymer was produced by submerged
cultivation of fourteen producing strains on medium with glycerol as a sole
carbon source. Each experiment was performed at a laboratory level under
aerobic conditions at 30°C and 150 rpm for 168 h and 240 h. Bioprocess
efficacy was estimated based on the xanthan quantity and its average
molecular weight that was selected as quality parameter. According to the
obtained results, it can be concluded that all applied strains have a
statistically significant effect on xanthan concentration in medium and on
its average molecular weight, while cultivation time significantly affect
the bioprocess efficacy only when biosynthesis is performed by Xanthomonas
strains isolated from crucifers. Further, when only Xanthomonas strains
isolated from crucifers are observed, statistically obtained data suggest
that the largest amount of the best quality xanthan in applied experimental
conditions can be accomplished by the cultivation of CB strain for 240 h. On
the other hand, when it comes to Xanthomonas strains isolated from pepper
leaves, the highest productivity is shown by PL 2, PL 4 and PL 5 strains
regardless of the cultivation time, while the PL 3 strain was responsible
for the synthesis of biopolymers with the highest average molecular weight.
The results obtained in this study represent valuable information for
development of biotechnological process for xanthan production on glycerol
containing media using new producing strain. |
| first_indexed | 2024-12-17T18:38:08Z |
| format | Article |
| id | doaj.art-979c93cd4716418d9bda5256eb96585c |
| institution | Directory Open Access Journal |
| issn | 1450-7188 2406-095X |
| language | English |
| last_indexed | 2024-12-17T18:38:08Z |
| publishDate | 2021-01-01 |
| publisher | Faculty of Technology, Novi Sad |
| record_format | Article |
| series | Acta Periodica Technologica |
| spelling | doaj.art-979c93cd4716418d9bda5256eb96585c2022-12-21T21:37:02ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882406-095X2021-01-0120215217318710.2298/APT2152173Z1450-71882152173ZThe effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing mediumZahović Ida E.0https://orcid.org/0000-0002-5735-5182Dodić Jelena M.1https://orcid.org/0000-0003-4858-1433Grahovac Jovana A.2https://orcid.org/0000-0001-9936-9665Grahovac Mila S.3https://orcid.org/0000-0001-9800-4103Trivunović Zorana Z.4https://orcid.org/0000-0002-2889-219XUniversity of Novi Sad, Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Agriculture, Department of Phytomedicine and Environmental Protection, Novi Sad, SerbiaUniversity of Novi Sad, Faculty of Technology Novi Sad, Department of Biotechnology and Pharmaceutical Engineering, Novi Sad, SerbiaIn this study, the influence of cultivation time on xanthan biosynthesis by different Xanthomonas strains, reference strain and crucifers’ and pepper leaves’ isolates, was examined. Biopolymer was produced by submerged cultivation of fourteen producing strains on medium with glycerol as a sole carbon source. Each experiment was performed at a laboratory level under aerobic conditions at 30°C and 150 rpm for 168 h and 240 h. Bioprocess efficacy was estimated based on the xanthan quantity and its average molecular weight that was selected as quality parameter. According to the obtained results, it can be concluded that all applied strains have a statistically significant effect on xanthan concentration in medium and on its average molecular weight, while cultivation time significantly affect the bioprocess efficacy only when biosynthesis is performed by Xanthomonas strains isolated from crucifers. Further, when only Xanthomonas strains isolated from crucifers are observed, statistically obtained data suggest that the largest amount of the best quality xanthan in applied experimental conditions can be accomplished by the cultivation of CB strain for 240 h. On the other hand, when it comes to Xanthomonas strains isolated from pepper leaves, the highest productivity is shown by PL 2, PL 4 and PL 5 strains regardless of the cultivation time, while the PL 3 strain was responsible for the synthesis of biopolymers with the highest average molecular weight. The results obtained in this study represent valuable information for development of biotechnological process for xanthan production on glycerol containing media using new producing strain.http://www.doiserbia.nb.rs/img/doi/1450-7188/2021/1450-71882152173Z.pdfbiotechnological productionbiopolymerxanthanxanthomonas strainscultivation time |
| spellingShingle | Zahović Ida E. Dodić Jelena M. Grahovac Jovana A. Grahovac Mila S. Trivunović Zorana Z. The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium Acta Periodica Technologica biotechnological production biopolymer xanthan xanthomonas strains cultivation time |
| title | The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium |
| title_full | The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium |
| title_fullStr | The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium |
| title_full_unstemmed | The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium |
| title_short | The effect of cultivation time on xanthan production by Xanthomonas spp. on glycerol containing medium |
| title_sort | effect of cultivation time on xanthan production by xanthomonas spp on glycerol containing medium |
| topic | biotechnological production biopolymer xanthan xanthomonas strains cultivation time |
| url | http://www.doiserbia.nb.rs/img/doi/1450-7188/2021/1450-71882152173Z.pdf |
| work_keys_str_mv | AT zahovicidae theeffectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT dodicjelenam theeffectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT grahovacjovanaa theeffectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT grahovacmilas theeffectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT trivunoviczoranaz theeffectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT zahovicidae effectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT dodicjelenam effectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT grahovacjovanaa effectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT grahovacmilas effectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium AT trivunoviczoranaz effectofcultivationtimeonxanthanproductionbyxanthomonasspponglycerolcontainingmedium |