Effect of Supplemental <i>Kluyveromyces marxianus</i> and <i>Pichia kudriavzevii</i> on Aflatoxin M<sub>1</sub> Excretion in Milk of Lactating Dairy Cows
The objective of this study was to determine the effect of supplementing <i>Kluyveromyces marxianus</i> CPY1, <i>K. marxianus</i> RSY5 and <i>Pichia kudriavzevii</i> YSY2 isolated from ruminal fluid of dairy cows on transfer of aflatoxin B<sub>1</sub>...
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MDPI AG
2020-04-01
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| Series: | Animals |
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| author | Malinee Intanoo Mallika B. Kongkeitkajorn Witaya Suriyasathaporn Yupin Phasuk John K. Bernard Virote Pattarajinda |
| author_facet | Malinee Intanoo Mallika B. Kongkeitkajorn Witaya Suriyasathaporn Yupin Phasuk John K. Bernard Virote Pattarajinda |
| author_sort | Malinee Intanoo |
| collection | DOAJ |
| description | The objective of this study was to determine the effect of supplementing <i>Kluyveromyces marxianus</i> CPY1, <i>K. marxianus</i> RSY5 and <i>Pichia kudriavzevii</i> YSY2 isolated from ruminal fluid of dairy cows on transfer of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) from feed into aflatoxin M<sub>1</sub> (AFM<sub>1</sub>) in milk, DMI, milk production and nutrient digestibility. Four multiparous Holsteins in mid-lactation were used in a 4 × 4 Latin square design trial consisting of 14 days in each experimental period for sample collection. Between each period, 14 clearance days prior to the next treatment were allowed to minimize carryover effects. In each treatment, subsequent supplementation of isolated yeast was compared, i.e., (1) control (without yeast supplementation), (2) <i>K. marxianus</i> CPY1 (K1Y), (3) <i>K. marxianus</i> RSY5 (K2Y) and (4) <i>P. kudriavzevii</i> YSY2 (PY). All diets contained 22.28 µg of AFB<sub>1</sub>/kg. Treatments were individually fed at the rate of 2 g/day (1 × 10<sup>9</sup> CFU/g) of yeast biomass or corn meal in the control group. Concentrations of AFM<sub>1</sub> in milk was reduced with yeast and averaged 1.54, 0.36, 0.43 and 0.51 µg/L for control, K1Y, K2Y and PY, respectively (<i>p</i> < 0.01). The transfer of AFB<sub>1</sub> from feed into AFM<sub>1</sub> in milk was higher in control compared with K1Y, K2Y and PY (7.26% vs. 1.18%, 1.44% and 1.69% respectively, <i>p</i> < 0.01). Supplementation of yeast also improved DMI and milk compositions, but no differences were observed in nutrient digestibility or milk yield among treatments. Concentration and yield of milk protein, fat, lactose, solid-not-fat (SNF) and total solids were greater in cows fed yeast compared with the control (<i>p</i> < 0.01). These results indicate that <i>K. marxianus</i> CPY1, RSY5 and <i>P. kudriavzevii</i> YSY2 shows promise as a dietary supplementation to detoxify AFB<sub>1</sub> and improve DMI and yield of milk components. |
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| spelling | doaj.art-979d84b1396f41ff987afb666e411d1e2023-11-19T22:02:49ZengMDPI AGAnimals2076-26152020-04-0110470910.3390/ani10040709Effect of Supplemental <i>Kluyveromyces marxianus</i> and <i>Pichia kudriavzevii</i> on Aflatoxin M<sub>1</sub> Excretion in Milk of Lactating Dairy CowsMalinee Intanoo0Mallika B. Kongkeitkajorn1Witaya Suriyasathaporn2Yupin Phasuk3John K. Bernard4Virote Pattarajinda5Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Feed Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Animal and Dairy Science, College of Agricultural and Environmental Science, The University of Georgia, Tifton, GA 31793, USADepartment of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, ThailandThe objective of this study was to determine the effect of supplementing <i>Kluyveromyces marxianus</i> CPY1, <i>K. marxianus</i> RSY5 and <i>Pichia kudriavzevii</i> YSY2 isolated from ruminal fluid of dairy cows on transfer of aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) from feed into aflatoxin M<sub>1</sub> (AFM<sub>1</sub>) in milk, DMI, milk production and nutrient digestibility. Four multiparous Holsteins in mid-lactation were used in a 4 × 4 Latin square design trial consisting of 14 days in each experimental period for sample collection. Between each period, 14 clearance days prior to the next treatment were allowed to minimize carryover effects. In each treatment, subsequent supplementation of isolated yeast was compared, i.e., (1) control (without yeast supplementation), (2) <i>K. marxianus</i> CPY1 (K1Y), (3) <i>K. marxianus</i> RSY5 (K2Y) and (4) <i>P. kudriavzevii</i> YSY2 (PY). All diets contained 22.28 µg of AFB<sub>1</sub>/kg. Treatments were individually fed at the rate of 2 g/day (1 × 10<sup>9</sup> CFU/g) of yeast biomass or corn meal in the control group. Concentrations of AFM<sub>1</sub> in milk was reduced with yeast and averaged 1.54, 0.36, 0.43 and 0.51 µg/L for control, K1Y, K2Y and PY, respectively (<i>p</i> < 0.01). The transfer of AFB<sub>1</sub> from feed into AFM<sub>1</sub> in milk was higher in control compared with K1Y, K2Y and PY (7.26% vs. 1.18%, 1.44% and 1.69% respectively, <i>p</i> < 0.01). Supplementation of yeast also improved DMI and milk compositions, but no differences were observed in nutrient digestibility or milk yield among treatments. Concentration and yield of milk protein, fat, lactose, solid-not-fat (SNF) and total solids were greater in cows fed yeast compared with the control (<i>p</i> < 0.01). These results indicate that <i>K. marxianus</i> CPY1, RSY5 and <i>P. kudriavzevii</i> YSY2 shows promise as a dietary supplementation to detoxify AFB<sub>1</sub> and improve DMI and yield of milk components.https://www.mdpi.com/2076-2615/10/4/709aflatoxinsmycotoxinsyeastsfeed supplementdairy cows |
| spellingShingle | Malinee Intanoo Mallika B. Kongkeitkajorn Witaya Suriyasathaporn Yupin Phasuk John K. Bernard Virote Pattarajinda Effect of Supplemental <i>Kluyveromyces marxianus</i> and <i>Pichia kudriavzevii</i> on Aflatoxin M<sub>1</sub> Excretion in Milk of Lactating Dairy Cows Animals aflatoxins mycotoxins yeasts feed supplement dairy cows |
| title | Effect of Supplemental <i>Kluyveromyces marxianus</i> and <i>Pichia kudriavzevii</i> on Aflatoxin M<sub>1</sub> Excretion in Milk of Lactating Dairy Cows |
| title_full | Effect of Supplemental <i>Kluyveromyces marxianus</i> and <i>Pichia kudriavzevii</i> on Aflatoxin M<sub>1</sub> Excretion in Milk of Lactating Dairy Cows |
| title_fullStr | Effect of Supplemental <i>Kluyveromyces marxianus</i> and <i>Pichia kudriavzevii</i> on Aflatoxin M<sub>1</sub> Excretion in Milk of Lactating Dairy Cows |
| title_full_unstemmed | Effect of Supplemental <i>Kluyveromyces marxianus</i> and <i>Pichia kudriavzevii</i> on Aflatoxin M<sub>1</sub> Excretion in Milk of Lactating Dairy Cows |
| title_short | Effect of Supplemental <i>Kluyveromyces marxianus</i> and <i>Pichia kudriavzevii</i> on Aflatoxin M<sub>1</sub> Excretion in Milk of Lactating Dairy Cows |
| title_sort | effect of supplemental i kluyveromyces marxianus i and i pichia kudriavzevii i on aflatoxin m sub 1 sub excretion in milk of lactating dairy cows |
| topic | aflatoxins mycotoxins yeasts feed supplement dairy cows |
| url | https://www.mdpi.com/2076-2615/10/4/709 |
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