Identification and characterization of a novel nematode pan allergen (NPA) from Wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia (TPE).
<h4>Background</h4>Tropical pulmonary eosinophilia (TPE) is a chronic respiratory syndrome associated with Lymphatic Filariasis (LF), a tropical parasitic infection of the human, transmitted by mosquitoes. The larval form of LF (microfilariae) are trapped in the lungs of TPE subjects hav...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-02-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011972&type=printable |
| _version_ | 1797279625191096320 |
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| author | Samuel Christopher Katru Anand Setty Balakrishnan Gnanasekar Munirathinam Azadeh Hadadianpour Scott A Smith Ramaswamy Kalyanasundaram |
| author_facet | Samuel Christopher Katru Anand Setty Balakrishnan Gnanasekar Munirathinam Azadeh Hadadianpour Scott A Smith Ramaswamy Kalyanasundaram |
| author_sort | Samuel Christopher Katru |
| collection | DOAJ |
| description | <h4>Background</h4>Tropical pulmonary eosinophilia (TPE) is a chronic respiratory syndrome associated with Lymphatic Filariasis (LF), a tropical parasitic infection of the human, transmitted by mosquitoes. The larval form of LF (microfilariae) are trapped in the lungs of TPE subjects have a major role in initiating the TPE syndrome. To date, there are no reports on the potential allergen that is responsible for generating parasite-specific IgE in TPE.<h4>Methodology/principal findings</h4>In this project, we screened a cDNA expression library of the microfilarial stages of Wuchereria bancrofti with monoclonal IgE antibodies prepared from subjects with clinical filarial infections. Our studies identified a novel molecule that showed significant sequence similarity to an allergen. A blast analysis showed the presence of similar proteins in a number of nematodes parasites. Thus, we named this molecule as Nematode Pan Allergen (NPA). Subsequent functional analysis showed that NPA is a potent allergen that can cause release of histamine from mast cells, induce secretion of proinflammatory cytokines from alveolar macrophages and promote accumulation of eosinophils in the tissue, all of which occur in TPE lungs.<h4>Conclusions/significance</h4>Based on our results, we conclude that the NPA protein secreted by the microfilariae of W. bancrofti may play a significant role in the pathology of TPE syndrome in LF infected individuals. Further studies on this molecule can help design an approach to neutralize the NPA in an attempt to reduce the pathology associated with TPE in LF infected subjects. |
| first_indexed | 2024-03-07T16:28:42Z |
| format | Article |
| id | doaj.art-979dd8a2163e4374acc1fe2a6684d845 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-03-07T16:28:42Z |
| publishDate | 2024-02-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-979dd8a2163e4374acc1fe2a6684d8452024-03-03T13:44:37ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352024-02-01182e001197210.1371/journal.pntd.0011972Identification and characterization of a novel nematode pan allergen (NPA) from Wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia (TPE).Samuel Christopher KatruAnand Setty BalakrishnanGnanasekar MunirathinamAzadeh HadadianpourScott A SmithRamaswamy Kalyanasundaram<h4>Background</h4>Tropical pulmonary eosinophilia (TPE) is a chronic respiratory syndrome associated with Lymphatic Filariasis (LF), a tropical parasitic infection of the human, transmitted by mosquitoes. The larval form of LF (microfilariae) are trapped in the lungs of TPE subjects have a major role in initiating the TPE syndrome. To date, there are no reports on the potential allergen that is responsible for generating parasite-specific IgE in TPE.<h4>Methodology/principal findings</h4>In this project, we screened a cDNA expression library of the microfilarial stages of Wuchereria bancrofti with monoclonal IgE antibodies prepared from subjects with clinical filarial infections. Our studies identified a novel molecule that showed significant sequence similarity to an allergen. A blast analysis showed the presence of similar proteins in a number of nematodes parasites. Thus, we named this molecule as Nematode Pan Allergen (NPA). Subsequent functional analysis showed that NPA is a potent allergen that can cause release of histamine from mast cells, induce secretion of proinflammatory cytokines from alveolar macrophages and promote accumulation of eosinophils in the tissue, all of which occur in TPE lungs.<h4>Conclusions/significance</h4>Based on our results, we conclude that the NPA protein secreted by the microfilariae of W. bancrofti may play a significant role in the pathology of TPE syndrome in LF infected individuals. Further studies on this molecule can help design an approach to neutralize the NPA in an attempt to reduce the pathology associated with TPE in LF infected subjects.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011972&type=printable |
| spellingShingle | Samuel Christopher Katru Anand Setty Balakrishnan Gnanasekar Munirathinam Azadeh Hadadianpour Scott A Smith Ramaswamy Kalyanasundaram Identification and characterization of a novel nematode pan allergen (NPA) from Wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia (TPE). PLoS Neglected Tropical Diseases |
| title | Identification and characterization of a novel nematode pan allergen (NPA) from Wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia (TPE). |
| title_full | Identification and characterization of a novel nematode pan allergen (NPA) from Wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia (TPE). |
| title_fullStr | Identification and characterization of a novel nematode pan allergen (NPA) from Wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia (TPE). |
| title_full_unstemmed | Identification and characterization of a novel nematode pan allergen (NPA) from Wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia (TPE). |
| title_short | Identification and characterization of a novel nematode pan allergen (NPA) from Wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia (TPE). |
| title_sort | identification and characterization of a novel nematode pan allergen npa from wuchereria bancrofti and their potential role in human filarial tropical pulmonary eosinophilia tpe |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0011972&type=printable |
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