Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responses
Abstract Drought is the main limiting factor for plant growth in karst areas with a fragile ecological environment. Cinnamomum migao H.W. Li is an endemic medicinal woody plant present in the karst areas of southwestern China, and it is endangered due to poor drought tolerance. Arbuscular mycorrhiza...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-07-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.9091 |
| _version_ | 1828359433437052928 |
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| author | Qiuxiao Yan Xiangying Li Xuefeng Xiao Jingzhong Chen Jiming Liu Changhu Lin Ruiting Guan Daoping Wang |
| author_facet | Qiuxiao Yan Xiangying Li Xuefeng Xiao Jingzhong Chen Jiming Liu Changhu Lin Ruiting Guan Daoping Wang |
| author_sort | Qiuxiao Yan |
| collection | DOAJ |
| description | Abstract Drought is the main limiting factor for plant growth in karst areas with a fragile ecological environment. Cinnamomum migao H.W. Li is an endemic medicinal woody plant present in the karst areas of southwestern China, and it is endangered due to poor drought tolerance. Arbuscular mycorrhizal fungi (AMF) are known to enhance the drought tolerance of plants. However, few studies have examined the contribution of AMF in improving the drought tolerance of C. migao seedlings. Therefore, we conducted a series of experiments to determine whether a single inoculation and coinoculation of AMF (Claroideoglomus lamellosum and Claroideoglomus etunicatum) enhanced the drought tolerance of C. migao. Furthermore, we compared the effects of single inoculation and coinoculation with different inoculum sizes (20, 40, 60, and 100 g; four replicates per treatment) on mycorrhizal colonization rate, plant growth, photosynthetic parameters, antioxidant enzyme activity, and malondialdehyde (MDA) and osmoregulatory substance contents. The results showed that compared with nonmycorrhizal plants, AMF colonization significantly improved plant growing status; net photosynthetic rate; superoxide dismutase, catalase, and peroxidase activities; and soluble sugar, soluble protein, and proline contents. Furthermore, AMF colonization increased relative water content and reduced MDA content in cells. These combined cumulative effects of AMF symbiosis ultimately enhanced the drought tolerance of seedlings and were closely related to the inoculum size. With an increase in inoculum size, the growth rate and drought tolerance of plants first increased and then decreased. The damage caused by drought stress could be reduced by inoculating 40–60 g of AMF, and the effect of coinoculation was significantly better than that of single inoculation at 60 g of AMF, while the effect was opposite at 40 g of AMF. Additionally, the interaction between AMF and inoculum sizes had a significant effect on drought tolerance. In conclusion, the inoculation of the AMF (Cl. lamellosum and Cl. etunicatum) improved photosynthesis, activated antioxidant enzymes, regulated cell osmotic state, and enhanced the drought tolerance of C. migao, enabling its growth in fragile ecological environments. |
| first_indexed | 2024-04-14T03:42:29Z |
| format | Article |
| id | doaj.art-979f49b4f87047a6a4fa979ea4b9fefa |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-04-14T03:42:29Z |
| publishDate | 2022-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-979f49b4f87047a6a4fa979ea4b9fefa2022-12-22T02:14:28ZengWileyEcology and Evolution2045-77582022-07-01127n/an/a10.1002/ece3.9091Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responsesQiuxiao Yan0Xiangying Li1Xuefeng Xiao2Jingzhong Chen3Jiming Liu4Changhu Lin5Ruiting Guan6Daoping Wang7Department of Ecology, College of Forestry Guizhou University Guiyang ChinaInstitute of New Rural Development Guizhou University Guiyang ChinaDepartment of Ecology, College of Forestry Guizhou University Guiyang ChinaDepartment of Ecology, College of Forestry Guizhou University Guiyang ChinaDepartment of Ecology, College of Forestry Guizhou University Guiyang ChinaDepartment of Labor Health and Environmental Hygiene, School of Public Health Guizhou Medical University Guiyang ChinaDepartment of Ecology, College of Forestry Guizhou University Guiyang ChinaThe Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences Guiyang ChinaAbstract Drought is the main limiting factor for plant growth in karst areas with a fragile ecological environment. Cinnamomum migao H.W. Li is an endemic medicinal woody plant present in the karst areas of southwestern China, and it is endangered due to poor drought tolerance. Arbuscular mycorrhizal fungi (AMF) are known to enhance the drought tolerance of plants. However, few studies have examined the contribution of AMF in improving the drought tolerance of C. migao seedlings. Therefore, we conducted a series of experiments to determine whether a single inoculation and coinoculation of AMF (Claroideoglomus lamellosum and Claroideoglomus etunicatum) enhanced the drought tolerance of C. migao. Furthermore, we compared the effects of single inoculation and coinoculation with different inoculum sizes (20, 40, 60, and 100 g; four replicates per treatment) on mycorrhizal colonization rate, plant growth, photosynthetic parameters, antioxidant enzyme activity, and malondialdehyde (MDA) and osmoregulatory substance contents. The results showed that compared with nonmycorrhizal plants, AMF colonization significantly improved plant growing status; net photosynthetic rate; superoxide dismutase, catalase, and peroxidase activities; and soluble sugar, soluble protein, and proline contents. Furthermore, AMF colonization increased relative water content and reduced MDA content in cells. These combined cumulative effects of AMF symbiosis ultimately enhanced the drought tolerance of seedlings and were closely related to the inoculum size. With an increase in inoculum size, the growth rate and drought tolerance of plants first increased and then decreased. The damage caused by drought stress could be reduced by inoculating 40–60 g of AMF, and the effect of coinoculation was significantly better than that of single inoculation at 60 g of AMF, while the effect was opposite at 40 g of AMF. Additionally, the interaction between AMF and inoculum sizes had a significant effect on drought tolerance. In conclusion, the inoculation of the AMF (Cl. lamellosum and Cl. etunicatum) improved photosynthesis, activated antioxidant enzymes, regulated cell osmotic state, and enhanced the drought tolerance of C. migao, enabling its growth in fragile ecological environments.https://doi.org/10.1002/ece3.9091antioxidant systemarbuscular mycorrhizal fungiCinnamomum migaodrought stressosmotic adjustmentphotosynthesis |
| spellingShingle | Qiuxiao Yan Xiangying Li Xuefeng Xiao Jingzhong Chen Jiming Liu Changhu Lin Ruiting Guan Daoping Wang Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responses Ecology and Evolution antioxidant system arbuscular mycorrhizal fungi Cinnamomum migao drought stress osmotic adjustment photosynthesis |
| title | Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responses |
| title_full | Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responses |
| title_fullStr | Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responses |
| title_full_unstemmed | Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responses |
| title_short | Arbuscular mycorrhizal fungi improve the growth and drought tolerance of Cinnamomum migao by enhancing physio‐biochemical responses |
| title_sort | arbuscular mycorrhizal fungi improve the growth and drought tolerance of cinnamomum migao by enhancing physio biochemical responses |
| topic | antioxidant system arbuscular mycorrhizal fungi Cinnamomum migao drought stress osmotic adjustment photosynthesis |
| url | https://doi.org/10.1002/ece3.9091 |
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