Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (<i>Solanum tuberosum</i> L.)
Drought represents a major abiotic stress factor negatively affecting growth, yield and tuber quality of potatoes. Quantitative trait locus (QTL) analyses were performed in cultivated potatoes for drought tolerance index DRYM (deviation of relative starch yield from the experimental median), tuber s...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Christina Schumacher Susanne Thümecke Florian Schilling Karin Köhl Joachim Kopka Heike Sprenger Dirk Karl Hincha Dirk Walther Sylvia Seddig Rolf Peters Ellen Zuther Manuela Haas Renate Horn |
| author_facet | Christina Schumacher Susanne Thümecke Florian Schilling Karin Köhl Joachim Kopka Heike Sprenger Dirk Karl Hincha Dirk Walther Sylvia Seddig Rolf Peters Ellen Zuther Manuela Haas Renate Horn |
| author_sort | Christina Schumacher |
| collection | DOAJ |
| description | Drought represents a major abiotic stress factor negatively affecting growth, yield and tuber quality of potatoes. Quantitative trait locus (QTL) analyses were performed in cultivated potatoes for drought tolerance index DRYM (deviation of relative starch yield from the experimental median), tuber starch content, tuber starch yield, tuber fresh weight, selected transcripts and metabolites under control and drought stress conditions. Eight genomic regions of major interest for drought tolerance were identified, three representing standalone DRYM QTL. Candidate genes, e.g., from signaling pathways for ethylene, abscisic acid and brassinosteroids, and genes encoding cell wall remodeling enzymes were identified within DRYM QTL. Co-localizations of DRYM QTL and QTL for tuber starch content, tuber starch yield and tuber fresh weight with underlying genes of the carbohydrate metabolism were observed. Overlaps of DRYM QTL with metabolite QTL for ribitol or galactinol may indicate trade-offs between starch and compatible solute biosynthesis. Expression QTL confirmed the drought stress relevance of selected transcripts by overlaps with DRYM QTL. Bulked segregant analyses combined with next-generation sequencing (BSAseq) were used to identify mutations in genes under the DRYM QTL on linkage group 3. Future analyses of identified genes for drought tolerance will give a better insight into drought tolerance in potatoes. |
| first_indexed | 2024-03-10T10:40:12Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:40:12Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-97a25f02e70f404cb2fb24f5cef634b12023-11-21T23:02:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012211612310.3390/ijms22116123Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (<i>Solanum tuberosum</i> L.)Christina Schumacher0Susanne Thümecke1Florian Schilling2Karin Köhl3Joachim Kopka4Heike Sprenger5Dirk Karl Hincha6Dirk Walther7Sylvia Seddig8Rolf Peters9Ellen Zuther10Manuela Haas11Renate Horn12Department of Plant Genetics, Institute of Biological Sciences, University of Rostock, Albert-Einstein-Str. 3, 18059 Rostock, GermanyDepartment of Plant Genetics, Institute of Biological Sciences, University of Rostock, Albert-Einstein-Str. 3, 18059 Rostock, GermanyDepartment of Plant Genetics, Institute of Biological Sciences, University of Rostock, Albert-Einstein-Str. 3, 18059 Rostock, GermanyMax Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, GermanyMax Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, GermanyMax Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, GermanyMax Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, GermanyMax Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, GermanyInstitute for Resistance Research and Stress Tolerance, Julius-Kühn Institut, Federal Research Centre for Cultivated Plants, Rudolf-Schick-Platz 3, 18190 Sanitz, GermanyLandwirtschaftskammer Niedersachsen, Dethlingen 14, 29633 Munster, GermanyMax Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, GermanyMax Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, GermanyDepartment of Plant Genetics, Institute of Biological Sciences, University of Rostock, Albert-Einstein-Str. 3, 18059 Rostock, GermanyDrought represents a major abiotic stress factor negatively affecting growth, yield and tuber quality of potatoes. Quantitative trait locus (QTL) analyses were performed in cultivated potatoes for drought tolerance index DRYM (deviation of relative starch yield from the experimental median), tuber starch content, tuber starch yield, tuber fresh weight, selected transcripts and metabolites under control and drought stress conditions. Eight genomic regions of major interest for drought tolerance were identified, three representing standalone DRYM QTL. Candidate genes, e.g., from signaling pathways for ethylene, abscisic acid and brassinosteroids, and genes encoding cell wall remodeling enzymes were identified within DRYM QTL. Co-localizations of DRYM QTL and QTL for tuber starch content, tuber starch yield and tuber fresh weight with underlying genes of the carbohydrate metabolism were observed. Overlaps of DRYM QTL with metabolite QTL for ribitol or galactinol may indicate trade-offs between starch and compatible solute biosynthesis. Expression QTL confirmed the drought stress relevance of selected transcripts by overlaps with DRYM QTL. Bulked segregant analyses combined with next-generation sequencing (BSAseq) were used to identify mutations in genes under the DRYM QTL on linkage group 3. Future analyses of identified genes for drought tolerance will give a better insight into drought tolerance in potatoes.https://www.mdpi.com/1422-0067/22/11/6123drought toleranceethylenebrassinosteroidscell walltuber starch contenttuber starch yield |
| spellingShingle | Christina Schumacher Susanne Thümecke Florian Schilling Karin Köhl Joachim Kopka Heike Sprenger Dirk Karl Hincha Dirk Walther Sylvia Seddig Rolf Peters Ellen Zuther Manuela Haas Renate Horn Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (<i>Solanum tuberosum</i> L.) International Journal of Molecular Sciences drought tolerance ethylene brassinosteroids cell wall tuber starch content tuber starch yield |
| title | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (<i>Solanum tuberosum</i> L.) |
| title_full | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (<i>Solanum tuberosum</i> L.) |
| title_fullStr | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (<i>Solanum tuberosum</i> L.) |
| title_full_unstemmed | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (<i>Solanum tuberosum</i> L.) |
| title_short | Genome-Wide Approach to Identify Quantitative Trait Loci for Drought Tolerance in Tetraploid Potato (<i>Solanum tuberosum</i> L.) |
| title_sort | genome wide approach to identify quantitative trait loci for drought tolerance in tetraploid potato i solanum tuberosum i l |
| topic | drought tolerance ethylene brassinosteroids cell wall tuber starch content tuber starch yield |
| url | https://www.mdpi.com/1422-0067/22/11/6123 |
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