CX<sub>3</sub>CR1 Mediates the Development of Monocyte-Derived Dendritic Cells during Hepatic Inflammation
Recent evidence suggests that hepatic dendritic cells (HDCs) contribute to the evolution of chronic liver diseases. However, the HDC subsets involved and the mechanisms driving these responses are still poorly understood. In this study, we have investigated the role of the fractalkine receptor CX<...
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MDPI AG
2019-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/8/9/1099 |
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| author | Salvatore Sutti Stefania Bruzzì Felix Heymann Anke Liepelt Oliver Krenkel Alberto Toscani Naresh Naik Ramavath Diego Cotella Emanuele Albano Frank Tacke |
| author_facet | Salvatore Sutti Stefania Bruzzì Felix Heymann Anke Liepelt Oliver Krenkel Alberto Toscani Naresh Naik Ramavath Diego Cotella Emanuele Albano Frank Tacke |
| author_sort | Salvatore Sutti |
| collection | DOAJ |
| description | Recent evidence suggests that hepatic dendritic cells (HDCs) contribute to the evolution of chronic liver diseases. However, the HDC subsets involved and the mechanisms driving these responses are still poorly understood. In this study, we have investigated the role of the fractalkine receptor CX<sub>3</sub>CR1 in modulating monocyte-derived dendritic cell (moDC) differentiation during liver inflammation. The phenotype of HDC and functional relevance of CX<sub>3</sub>CR1 was assessed in mice following necro-inflammatory liver injury induced by the hepatotoxic agent carbon tetrachloride (CCl<sub>4</sub>) and in steatohepatitis caused by a methionine/choline-deficient (MCD) diet. In both the experimental models, hepatic inflammation was associated with a massive expansion of CD11c<sup>+</sup>/MHCII<sup>high</sup>/CD11b<sup>+</sup> myeloid HDCs. These cells also expressed the monocyte markers Ly6C, chemokine (C-C Motif) receptor 2 (CCR2), F4/80 and CD88, along with CX<sub>3</sub>CR1, allowing their tentative identification as moDCs. Mice defective in CX<sub>3</sub>CR1 showed a reduction in liver-moDC recruitment following CCl<sub>4</sub> poisoning in parallel with a defective maturation of monocytes into moDCs. The lack of CX<sub>3</sub>CR1 also affected moDC differentiation from bone marrow myeloid cells induced by granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) in vitro. In wild-type mice, treatment with the CX<sub>3</sub>CR1 antagonist CX3-AT (150 µg, i.p.) 24 h after CCl<sub>4</sub> administration reduced liver moDC<sub>S</sub> and significantly ameliorated hepatic injury and inflammation. Altogether, these results highlight the possible involvement of moDCs in promoting hepatic inflammation following liver injury and indicated a novel role of CX<sub>3</sub>CL1/CX<sub>3</sub>CR1 dyad in driving the differentiation of hepatic moDCs. |
| first_indexed | 2024-03-12T08:14:04Z |
| format | Article |
| id | doaj.art-97a670bf0c61454db67261d87b998d2c |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T08:14:04Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-97a670bf0c61454db67261d87b998d2c2023-09-02T19:01:17ZengMDPI AGCells2073-44092019-09-0189109910.3390/cells8091099cells8091099CX<sub>3</sub>CR1 Mediates the Development of Monocyte-Derived Dendritic Cells during Hepatic InflammationSalvatore Sutti0Stefania Bruzzì1Felix Heymann2Anke Liepelt3Oliver Krenkel4Alberto Toscani5Naresh Naik Ramavath6Diego Cotella7Emanuele Albano8Frank Tacke9Department of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University “Amedeo Avogadro” of East Piedmont, Via Solaroli 17, 28100 Novara, ItalyDepartment of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University “Amedeo Avogadro” of East Piedmont, Via Solaroli 17, 28100 Novara, ItalyDepartment of Hepatology and Gastroenterology, Charité University Medical Center Berlin, 10117 Berlin, GermanyDepartment of Medicine III, RWTH-University Hospital Aachen, 52074 Aachen, GermanyDepartment of Medicine III, RWTH-University Hospital Aachen, 52074 Aachen, GermanyDepartment of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University “Amedeo Avogadro” of East Piedmont, Via Solaroli 17, 28100 Novara, ItalyDepartment of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University “Amedeo Avogadro” of East Piedmont, Via Solaroli 17, 28100 Novara, ItalyDepartment of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University “Amedeo Avogadro” of East Piedmont, Via Solaroli 17, 28100 Novara, ItalyDepartment of Health Sciences and Interdisciplinary Research Centre for Autoimmune Diseases, University “Amedeo Avogadro” of East Piedmont, Via Solaroli 17, 28100 Novara, ItalyDepartment of Hepatology and Gastroenterology, Charité University Medical Center Berlin, 10117 Berlin, GermanyRecent evidence suggests that hepatic dendritic cells (HDCs) contribute to the evolution of chronic liver diseases. However, the HDC subsets involved and the mechanisms driving these responses are still poorly understood. In this study, we have investigated the role of the fractalkine receptor CX<sub>3</sub>CR1 in modulating monocyte-derived dendritic cell (moDC) differentiation during liver inflammation. The phenotype of HDC and functional relevance of CX<sub>3</sub>CR1 was assessed in mice following necro-inflammatory liver injury induced by the hepatotoxic agent carbon tetrachloride (CCl<sub>4</sub>) and in steatohepatitis caused by a methionine/choline-deficient (MCD) diet. In both the experimental models, hepatic inflammation was associated with a massive expansion of CD11c<sup>+</sup>/MHCII<sup>high</sup>/CD11b<sup>+</sup> myeloid HDCs. These cells also expressed the monocyte markers Ly6C, chemokine (C-C Motif) receptor 2 (CCR2), F4/80 and CD88, along with CX<sub>3</sub>CR1, allowing their tentative identification as moDCs. Mice defective in CX<sub>3</sub>CR1 showed a reduction in liver-moDC recruitment following CCl<sub>4</sub> poisoning in parallel with a defective maturation of monocytes into moDCs. The lack of CX<sub>3</sub>CR1 also affected moDC differentiation from bone marrow myeloid cells induced by granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) in vitro. In wild-type mice, treatment with the CX<sub>3</sub>CR1 antagonist CX3-AT (150 µg, i.p.) 24 h after CCl<sub>4</sub> administration reduced liver moDC<sub>S</sub> and significantly ameliorated hepatic injury and inflammation. Altogether, these results highlight the possible involvement of moDCs in promoting hepatic inflammation following liver injury and indicated a novel role of CX<sub>3</sub>CL1/CX<sub>3</sub>CR1 dyad in driving the differentiation of hepatic moDCs.https://www.mdpi.com/2073-4409/8/9/1099dendritic cellsliver injuryfractalkinecarbon tetrachloride |
| spellingShingle | Salvatore Sutti Stefania Bruzzì Felix Heymann Anke Liepelt Oliver Krenkel Alberto Toscani Naresh Naik Ramavath Diego Cotella Emanuele Albano Frank Tacke CX<sub>3</sub>CR1 Mediates the Development of Monocyte-Derived Dendritic Cells during Hepatic Inflammation Cells dendritic cells liver injury fractalkine carbon tetrachloride |
| title | CX<sub>3</sub>CR1 Mediates the Development of Monocyte-Derived Dendritic Cells during Hepatic Inflammation |
| title_full | CX<sub>3</sub>CR1 Mediates the Development of Monocyte-Derived Dendritic Cells during Hepatic Inflammation |
| title_fullStr | CX<sub>3</sub>CR1 Mediates the Development of Monocyte-Derived Dendritic Cells during Hepatic Inflammation |
| title_full_unstemmed | CX<sub>3</sub>CR1 Mediates the Development of Monocyte-Derived Dendritic Cells during Hepatic Inflammation |
| title_short | CX<sub>3</sub>CR1 Mediates the Development of Monocyte-Derived Dendritic Cells during Hepatic Inflammation |
| title_sort | cx sub 3 sub cr1 mediates the development of monocyte derived dendritic cells during hepatic inflammation |
| topic | dendritic cells liver injury fractalkine carbon tetrachloride |
| url | https://www.mdpi.com/2073-4409/8/9/1099 |
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