A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us?
Taeniosis is a neglected disease, particularly in developing countries, and is caused by infection with the adult tapeworm of either Taenia solium, Taenia saginata, and Taenia asiatica. Of these, T. solium is of primary concern due to the potential for cysticercosis should T. solium eggs be ingested...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Food and Waterborne Parasitology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405676621000275 |
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| author | Luis Enrique Jerez Puebla Fidel A. Núñez Fernández Jorge Fraga Nodarse Raul Cordovi Prado Iraís Atencio Millán Iredys Cruz Rodríguez Rigoberto Fimia Duarte Marina del Carmen Sánchez Romero Sahily de la Caridad Ortega Medina Ubaldo del Risco Lissette Pérez Santos Lucy J. Robertson |
| author_facet | Luis Enrique Jerez Puebla Fidel A. Núñez Fernández Jorge Fraga Nodarse Raul Cordovi Prado Iraís Atencio Millán Iredys Cruz Rodríguez Rigoberto Fimia Duarte Marina del Carmen Sánchez Romero Sahily de la Caridad Ortega Medina Ubaldo del Risco Lissette Pérez Santos Lucy J. Robertson |
| author_sort | Luis Enrique Jerez Puebla |
| collection | DOAJ |
| description | Taeniosis is a neglected disease, particularly in developing countries, and is caused by infection with the adult tapeworm of either Taenia solium, Taenia saginata, and Taenia asiatica. Of these, T. solium is of primary concern due to the potential for cysticercosis should T. solium eggs be ingested. In Cuba, all cases of taeniosis are assumed to be caused by T. saginata, although some cases of cysticercosis have been documented. It is therefore important to gain further insights regarding the species causing taeniosis in Cuba, especially as diagnostic records indicate an increasing incidence, with the highest number of cases reported in 2020. In this study, we analysed 37 Taenia-positive faecal samples (or proglottids isolated from faecal samples) from the period 2001 until 2020 from all regions of the country. Genomic DNA was extracted from the samples, which had been stored in 10% formalin, using the QIAamp Tissue Kit. Species identification was carried out by duplex real-time PCR targeting the mitochondrial DNA. All cases were found to be T. saginata, and sequence analysis of three isolates confirmed the identification of this species. Our data do not provide any evidence that T. solium currently occurs in Cuba. However, given the relatively low number of samples analysed here, that the parasite may be imported with visitors or travellers who have been in endemic countries, and that taeniosis has relatively mild symptoms and thus infected patients may not seek medical attention, we recommend species determination for all taeniosis cases reported in Cuba. |
| first_indexed | 2024-12-22T20:44:29Z |
| format | Article |
| id | doaj.art-97a702bd33544f1bb6c2811eda6c7683 |
| institution | Directory Open Access Journal |
| issn | 2405-6766 |
| language | English |
| last_indexed | 2024-12-22T20:44:29Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food and Waterborne Parasitology |
| spelling | doaj.art-97a702bd33544f1bb6c2811eda6c76832022-12-21T18:13:16ZengElsevierFood and Waterborne Parasitology2405-67662021-12-0125e00136A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us?Luis Enrique Jerez Puebla0Fidel A. Núñez Fernández1Jorge Fraga Nodarse2Raul Cordovi Prado3Iraís Atencio Millán4Iredys Cruz Rodríguez5Rigoberto Fimia Duarte6Marina del Carmen Sánchez Romero7Sahily de la Caridad Ortega Medina8Ubaldo del Risco9Lissette Pérez Santos10Lucy J. Robertson11Departamento de Parasitología, Instituto ''Pedro Kourí'', Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, CubaDepartamento de Parasitología, Instituto ''Pedro Kourí'', Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, Cuba; Latin-American School of Medical Science, La Habana, CubaDepartamento de Parasitología, Instituto ''Pedro Kourí'', Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, CubaDepartamento de Parasitología, Instituto ''Pedro Kourí'', Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, CubaDepartamento de Parasitología, Instituto ''Pedro Kourí'', Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, CubaDepartamento de Parasitología, Instituto ''Pedro Kourí'', Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, CubaFaculty of Health Technology and Nursing, University of Medical Sciences of Villa Clara, CubaHygiene Provincial Centre of Microbiology and Epidemiology, Guantánamo Province, CubaHygiene Provincial Centre of Microbiology and Epidemiology, Cienfuegos Province, CubaHygiene Provincial Centre of Microbiology and Epidemiology, Camagüey Province, CubaDepartment of Virology, Tropical Medicine Institute ''Pedro Kourí'', Autopista Novia del Mediodía Km 6½ e/Autopista Nacional y Carretera Central, La Habana, CubaFaculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway; Corresponding author.Taeniosis is a neglected disease, particularly in developing countries, and is caused by infection with the adult tapeworm of either Taenia solium, Taenia saginata, and Taenia asiatica. Of these, T. solium is of primary concern due to the potential for cysticercosis should T. solium eggs be ingested. In Cuba, all cases of taeniosis are assumed to be caused by T. saginata, although some cases of cysticercosis have been documented. It is therefore important to gain further insights regarding the species causing taeniosis in Cuba, especially as diagnostic records indicate an increasing incidence, with the highest number of cases reported in 2020. In this study, we analysed 37 Taenia-positive faecal samples (or proglottids isolated from faecal samples) from the period 2001 until 2020 from all regions of the country. Genomic DNA was extracted from the samples, which had been stored in 10% formalin, using the QIAamp Tissue Kit. Species identification was carried out by duplex real-time PCR targeting the mitochondrial DNA. All cases were found to be T. saginata, and sequence analysis of three isolates confirmed the identification of this species. Our data do not provide any evidence that T. solium currently occurs in Cuba. However, given the relatively low number of samples analysed here, that the parasite may be imported with visitors or travellers who have been in endemic countries, and that taeniosis has relatively mild symptoms and thus infected patients may not seek medical attention, we recommend species determination for all taeniosis cases reported in Cuba.http://www.sciencedirect.com/science/article/pii/S2405676621000275CubaHuman infectionDiagnosisEpidemiologyMolecular confirmationTaenia |
| spellingShingle | Luis Enrique Jerez Puebla Fidel A. Núñez Fernández Jorge Fraga Nodarse Raul Cordovi Prado Iraís Atencio Millán Iredys Cruz Rodríguez Rigoberto Fimia Duarte Marina del Carmen Sánchez Romero Sahily de la Caridad Ortega Medina Ubaldo del Risco Lissette Pérez Santos Lucy J. Robertson A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us? Food and Waterborne Parasitology Cuba Human infection Diagnosis Epidemiology Molecular confirmation Taenia |
| title | A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us? |
| title_full | A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us? |
| title_fullStr | A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us? |
| title_full_unstemmed | A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us? |
| title_short | A retrospective study of Taenia spp. in Cuban patients: what does molecular analysis tell us? |
| title_sort | retrospective study of taenia spp in cuban patients what does molecular analysis tell us |
| topic | Cuba Human infection Diagnosis Epidemiology Molecular confirmation Taenia |
| url | http://www.sciencedirect.com/science/article/pii/S2405676621000275 |
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