In Vitro Preliminary Evaluation of Bacterial Attachment on Grooved and Smooth Healing Abutments
This in vitro preliminary study investigated the attachment of <i>Fusobacterium nucleatum</i> and <i>Porphyromonas gingivalis</i> on titanium alloy healing abutments, which differed in their surface macro-morphology: one was groove-marked while the other was completely smooth...
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MDPI AG
2020-06-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/13/4426 |
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| author | Ofer Moses Carlos E. Nemcovsky Israel Lewinstein Hasan Zoabi Miron Weinreb Shifra Levartovsky Shlomo Matalon |
| author_facet | Ofer Moses Carlos E. Nemcovsky Israel Lewinstein Hasan Zoabi Miron Weinreb Shifra Levartovsky Shlomo Matalon |
| author_sort | Ofer Moses |
| collection | DOAJ |
| description | This in vitro preliminary study investigated the attachment of <i>Fusobacterium nucleatum</i> and <i>Porphyromonas gingivalis</i> on titanium alloy healing abutments, which differed in their surface macro-morphology: one was groove-marked while the other was completely smooth. Altogether, twenty implant-healing abutments, ten of each macro-morphology, were evaluated with a single type of bacterial strain. Accordingly, four groups of five abutments each were created. The sterilized healing abutments with the cultured bacteria were placed under anaerobic conditions for 48 h at 37 °C. Afterwards, the abutments were examined with a scanning electron microscope, at a 2500x magnification. Attached bacteria were quantified in the four vertical quarters within the grooved abutments and in the two most coronal millimeters of the smooth abutments. The results were analyzed by applying two-way ANOVA, with square root transformation for a normal distribution. The bacterial attachment of both strains was statistically significantly larger in the grooved abutment areas than on the smooth surfaces (<i>p</i> ≤ 0.0001), twenty times so for <i>Porphyromonas gingivalis</i> and a hundred times so for <i>Fusobacterium nucleatum</i>. |
| first_indexed | 2024-03-10T18:51:33Z |
| format | Article |
| id | doaj.art-97ab2d9513d14986a7b2801f6e441da6 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-10T18:51:33Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-97ab2d9513d14986a7b2801f6e441da62023-11-20T05:06:52ZengMDPI AGApplied Sciences2076-34172020-06-011013442610.3390/app10134426In Vitro Preliminary Evaluation of Bacterial Attachment on Grooved and Smooth Healing AbutmentsOfer Moses0Carlos E. Nemcovsky1Israel Lewinstein2Hasan Zoabi3Miron Weinreb4Shifra Levartovsky5Shlomo Matalon6Department of Periodontology and Dental Implantology, School of Dental Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Periodontology and Dental Implantology, School of Dental Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Oral Rehabilitation, School of Dental Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Oral Biology, School of Dental Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Oral Biology, School of Dental Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Oral Rehabilitation, School of Dental Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelDepartment of Oral Rehabilitation, School of Dental Medicine, Tel Aviv University, Tel Aviv 6997801, IsraelThis in vitro preliminary study investigated the attachment of <i>Fusobacterium nucleatum</i> and <i>Porphyromonas gingivalis</i> on titanium alloy healing abutments, which differed in their surface macro-morphology: one was groove-marked while the other was completely smooth. Altogether, twenty implant-healing abutments, ten of each macro-morphology, were evaluated with a single type of bacterial strain. Accordingly, four groups of five abutments each were created. The sterilized healing abutments with the cultured bacteria were placed under anaerobic conditions for 48 h at 37 °C. Afterwards, the abutments were examined with a scanning electron microscope, at a 2500x magnification. Attached bacteria were quantified in the four vertical quarters within the grooved abutments and in the two most coronal millimeters of the smooth abutments. The results were analyzed by applying two-way ANOVA, with square root transformation for a normal distribution. The bacterial attachment of both strains was statistically significantly larger in the grooved abutment areas than on the smooth surfaces (<i>p</i> ≤ 0.0001), twenty times so for <i>Porphyromonas gingivalis</i> and a hundred times so for <i>Fusobacterium nucleatum</i>.https://www.mdpi.com/2076-3417/10/13/4426smooth healing abutmentgrooved healing abutmentbacterial growthSEM |
| spellingShingle | Ofer Moses Carlos E. Nemcovsky Israel Lewinstein Hasan Zoabi Miron Weinreb Shifra Levartovsky Shlomo Matalon In Vitro Preliminary Evaluation of Bacterial Attachment on Grooved and Smooth Healing Abutments Applied Sciences smooth healing abutment grooved healing abutment bacterial growth SEM |
| title | In Vitro Preliminary Evaluation of Bacterial Attachment on Grooved and Smooth Healing Abutments |
| title_full | In Vitro Preliminary Evaluation of Bacterial Attachment on Grooved and Smooth Healing Abutments |
| title_fullStr | In Vitro Preliminary Evaluation of Bacterial Attachment on Grooved and Smooth Healing Abutments |
| title_full_unstemmed | In Vitro Preliminary Evaluation of Bacterial Attachment on Grooved and Smooth Healing Abutments |
| title_short | In Vitro Preliminary Evaluation of Bacterial Attachment on Grooved and Smooth Healing Abutments |
| title_sort | in vitro preliminary evaluation of bacterial attachment on grooved and smooth healing abutments |
| topic | smooth healing abutment grooved healing abutment bacterial growth SEM |
| url | https://www.mdpi.com/2076-3417/10/13/4426 |
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