Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy
Alternating current scanning electrochemical microscopy (AC-SECM) is a powerful tool for characterizing the electrochemical reactivity of surfaces. Here, perturbation in the sample is induced by the alternating current and altered local potential is measured by the SECM probe. This technique has bee...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1063063/full |
| _version_ | 1811168418272903168 |
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| author | Varun Vyas Varun Vyas Niranjan G. Kotla Yury Rochev Anup Poudel Manus Biggs |
| author_facet | Varun Vyas Varun Vyas Niranjan G. Kotla Yury Rochev Anup Poudel Manus Biggs |
| author_sort | Varun Vyas |
| collection | DOAJ |
| description | Alternating current scanning electrochemical microscopy (AC-SECM) is a powerful tool for characterizing the electrochemical reactivity of surfaces. Here, perturbation in the sample is induced by the alternating current and altered local potential is measured by the SECM probe. This technique has been used to investigate many exotic a range of biological interfaces including live cells and tissues, as well as the corrosive degradation of various metallic surfaces, etc. In principle, AC-SECM imaging is derived from electrochemical impedance spectroscopy (EIS) which has been used for a century to describe interfacial and diffusive behaviour of molecules in solution or on a surface. Increasingly bioimpedance centric medical devices have become an important tool to detect evolution of tissue biochemistry. Predictive implications of measuring electrochemical changes within a tissue is one of the core concepts in developing minimally invasive and smart medical devices. In this study, cross sections of mice colon tissue were used for AC-SECM imaging. A 10 micron sized platinum probe was used for two-dimensional (2D) tan δ mapping of histological sections at a frequency of 10 kHz, Thereafter, multifrequency scans were performed at 100 Hz, 10 kHz, 300 kHz, and 900 kHz. Loss tangent (tan δ) mapping of mice colon revealed microscale regions within a tissue possessing a discrete tan δ signature. This tan δ map may be an immediate measure of physiological conditions in biological tissues. Multifrequency scans highlight subtle changes in protein or lipid composition as a function of frequency which was recorded as loss tangent maps. Impedance profile at different frequencies could also be used to identify optimal contrast for imaging and extracting the electrochemical signature specific for a tissue and its electrolyte. |
| first_indexed | 2024-04-10T16:26:00Z |
| format | Article |
| id | doaj.art-97af460539214af388bbc594ba227b08 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T16:26:00Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-97af460539214af388bbc594ba227b082023-02-09T05:09:21ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-02-011110.3389/fbioe.2023.10630631063063Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopyVarun Vyas0Varun Vyas1Niranjan G. Kotla2Yury Rochev3Anup Poudel4Manus Biggs5CÚRAM, SFI Research Centre for Medical Devices, University of Galway, Galway, IrelandCNRS, LIEC, Université de Lorraine, Nancy, FranceCÚRAM, SFI Research Centre for Medical Devices, University of Galway, Galway, IrelandCÚRAM, SFI Research Centre for Medical Devices, University of Galway, Galway, IrelandCÚRAM, SFI Research Centre for Medical Devices, University of Galway, Galway, IrelandCÚRAM, SFI Research Centre for Medical Devices, University of Galway, Galway, IrelandAlternating current scanning electrochemical microscopy (AC-SECM) is a powerful tool for characterizing the electrochemical reactivity of surfaces. Here, perturbation in the sample is induced by the alternating current and altered local potential is measured by the SECM probe. This technique has been used to investigate many exotic a range of biological interfaces including live cells and tissues, as well as the corrosive degradation of various metallic surfaces, etc. In principle, AC-SECM imaging is derived from electrochemical impedance spectroscopy (EIS) which has been used for a century to describe interfacial and diffusive behaviour of molecules in solution or on a surface. Increasingly bioimpedance centric medical devices have become an important tool to detect evolution of tissue biochemistry. Predictive implications of measuring electrochemical changes within a tissue is one of the core concepts in developing minimally invasive and smart medical devices. In this study, cross sections of mice colon tissue were used for AC-SECM imaging. A 10 micron sized platinum probe was used for two-dimensional (2D) tan δ mapping of histological sections at a frequency of 10 kHz, Thereafter, multifrequency scans were performed at 100 Hz, 10 kHz, 300 kHz, and 900 kHz. Loss tangent (tan δ) mapping of mice colon revealed microscale regions within a tissue possessing a discrete tan δ signature. This tan δ map may be an immediate measure of physiological conditions in biological tissues. Multifrequency scans highlight subtle changes in protein or lipid composition as a function of frequency which was recorded as loss tangent maps. Impedance profile at different frequencies could also be used to identify optimal contrast for imaging and extracting the electrochemical signature specific for a tissue and its electrolyte.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1063063/fullAC-SECMdielectric losstissuemultifrequency mappingEISimpedance |
| spellingShingle | Varun Vyas Varun Vyas Niranjan G. Kotla Yury Rochev Anup Poudel Manus Biggs Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy Frontiers in Bioengineering and Biotechnology AC-SECM dielectric loss tissue multifrequency mapping EIS impedance |
| title | Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy |
| title_full | Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy |
| title_fullStr | Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy |
| title_full_unstemmed | Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy |
| title_short | Multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy |
| title_sort | multifrequency dielectric mapping of fixed mice colon tissues in cell culture media via scanning electrochemical microscopy |
| topic | AC-SECM dielectric loss tissue multifrequency mapping EIS impedance |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1063063/full |
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