Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish
Excessive alcohol consumption causes the cellular and tissue damage. The toxic metabolites of ethanol are harmful to multiple organ systems, such as the central nervous system, skeletal muscles, and liver, and cause alcohol-induced diseases like cancer, as well as induce hepatotoxicity, and alcoholi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Animal Cells and Systems |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19768354.2021.1882565 |
| _version_ | 1818427050201972736 |
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| author | Youngsook Choi Heymin Seo Mina Cho Joohee Kim Hak Suk Chung Icksoo Lee Min Jung Kim |
| author_facet | Youngsook Choi Heymin Seo Mina Cho Joohee Kim Hak Suk Chung Icksoo Lee Min Jung Kim |
| author_sort | Youngsook Choi |
| collection | DOAJ |
| description | Excessive alcohol consumption causes the cellular and tissue damage. The toxic metabolites of ethanol are harmful to multiple organ systems, such as the central nervous system, skeletal muscles, and liver, and cause alcohol-induced diseases like cancer, as well as induce hepatotoxicity, and alcoholic myopathy. Alcohol exposure leads to a surge in hepatic alcohol metabolism and oxygen consumption, a decrease in hepatic ATP, and the rapid accumulation of lipid within hepatocytes. Several pathologies are closely linked to defective mitochondrial dynamics triggered by abnormal mitochondrial function and cellular homeostasis, raising the possibility that novel drugs targeting mitochondrial dynamics may have therapeutic potential in restoring cellular homeostasis in ethanol-induced hepatotoxicity. Rutin is a phytochemical polyphenol known to protect cells from cytotoxic chemicals. We investigated the effects of rutin on mitochondrial dynamics induced by ethanol. We found that rutin enhances mitochondrial dynamics by suppressing mitochondrial fission and restoring the balance of the mitochondrial dynamics. Mitochondrial elongation following rutin treatment of ethanol exposed cells was accompanied by reduced DRP1 expression. These data suggest that rutin plays an important role in remodeling of mitochondrial dynamics to alleviate hepatic steatosis and enhance mitochondrial function and cell viability. |
| first_indexed | 2024-12-14T14:39:34Z |
| format | Article |
| id | doaj.art-97b351010ad14f83ba5a3b313cf96fbe |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-12-14T14:39:34Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-97b351010ad14f83ba5a3b313cf96fbe2022-12-21T22:57:27ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852021-01-01251748110.1080/19768354.2021.18825651882565Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafishYoungsook Choi0Heymin Seo1Mina Cho2Joohee Kim3Hak Suk Chung4Icksoo Lee5Min Jung Kim6Sookmyung Women’s UniversitySookmyung Women’s UniversitySookmyung Women’s UniversitySookmyung Women’s UniversityKorea Institute of Science and TechnologyDankook UniversitySookmyung Women’s UniversityExcessive alcohol consumption causes the cellular and tissue damage. The toxic metabolites of ethanol are harmful to multiple organ systems, such as the central nervous system, skeletal muscles, and liver, and cause alcohol-induced diseases like cancer, as well as induce hepatotoxicity, and alcoholic myopathy. Alcohol exposure leads to a surge in hepatic alcohol metabolism and oxygen consumption, a decrease in hepatic ATP, and the rapid accumulation of lipid within hepatocytes. Several pathologies are closely linked to defective mitochondrial dynamics triggered by abnormal mitochondrial function and cellular homeostasis, raising the possibility that novel drugs targeting mitochondrial dynamics may have therapeutic potential in restoring cellular homeostasis in ethanol-induced hepatotoxicity. Rutin is a phytochemical polyphenol known to protect cells from cytotoxic chemicals. We investigated the effects of rutin on mitochondrial dynamics induced by ethanol. We found that rutin enhances mitochondrial dynamics by suppressing mitochondrial fission and restoring the balance of the mitochondrial dynamics. Mitochondrial elongation following rutin treatment of ethanol exposed cells was accompanied by reduced DRP1 expression. These data suggest that rutin plays an important role in remodeling of mitochondrial dynamics to alleviate hepatic steatosis and enhance mitochondrial function and cell viability.http://dx.doi.org/10.1080/19768354.2021.1882565rutinethanoldrp1zebrafishmitochondria |
| spellingShingle | Youngsook Choi Heymin Seo Mina Cho Joohee Kim Hak Suk Chung Icksoo Lee Min Jung Kim Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish Animal Cells and Systems rutin ethanol drp1 zebrafish mitochondria |
| title | Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish |
| title_full | Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish |
| title_fullStr | Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish |
| title_full_unstemmed | Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish |
| title_short | Rutin inhibits DRP1-mediated mitochondrial fission and prevents ethanol-induced hepatotoxicity in HepG2 cells and zebrafish |
| title_sort | rutin inhibits drp1 mediated mitochondrial fission and prevents ethanol induced hepatotoxicity in hepg2 cells and zebrafish |
| topic | rutin ethanol drp1 zebrafish mitochondria |
| url | http://dx.doi.org/10.1080/19768354.2021.1882565 |
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