Bacterial nanocellulose production from naphthalene
Summary Polycyclic aromatic compounds (PAHs) are toxic compounds that are released in the environment as a consequence of industrial activities. The restoration of PAH‐polluted sites considers the use of bacteria capable of degrading aromatic compounds to carbon dioxide and water. Here we characteri...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-07-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.13399 |
| _version_ | 1811290795574034432 |
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| author | Patricia Marín Sophie Marie Martirani‐Von Abercron Leire Urbina Daniel Pacheco‐Sánchez Mayra Alejandra Castañeda‐Cataña Aloña Retegi Arantxa Eceiza Silvia Marqués |
| author_facet | Patricia Marín Sophie Marie Martirani‐Von Abercron Leire Urbina Daniel Pacheco‐Sánchez Mayra Alejandra Castañeda‐Cataña Aloña Retegi Arantxa Eceiza Silvia Marqués |
| author_sort | Patricia Marín |
| collection | DOAJ |
| description | Summary Polycyclic aromatic compounds (PAHs) are toxic compounds that are released in the environment as a consequence of industrial activities. The restoration of PAH‐polluted sites considers the use of bacteria capable of degrading aromatic compounds to carbon dioxide and water. Here we characterize a new Xanthobacteraceae strain, Starkeya sp. strain N1B, previously isolated during enrichment under microaerophilic conditions, which is capable of using naphthalene crystals as the sole carbon source. The strain produced a structured biofilm when grown on naphthalene crystals, which had the shape of a half‐sphere organized over the crystal. Scanning electron microscopy (SEM) and GC‐MS analysis indicated that the biofilm was essentially made of cellulose, composed of several micron‐long nanofibrils of 60 nm diameter. A cellulosic biofilm was also formed when the cells grew with glucose as the carbon source. Fourier transformed infrared spectroscopy (FTIR) confirmed that the polymer was type I cellulose in both cases, although the crystallinity of the material greatly depended on the carbon source used for growth. Using genome mining and mutant analysis, we identified the genetic complements required for the transformation of naphthalene into cellulose, which seemed to have been successively acquired through horizontal gene transfer. The capacity to develop the biofilm around the crystal was found to be dispensable for growth when naphthalene was used as the carbon source, suggesting that the function of this structure is more intricate than initially thought. This is the first example of the use of toxic aromatic hydrocarbons as the carbon source for bacterial cellulose production. Application of this capacity would allow the remediation of a PAH into such a value‐added polymer with multiple biotechnological usages. |
| first_indexed | 2024-04-13T04:18:52Z |
| format | Article |
| id | doaj.art-97b8c514c6bd480b8eab36003798445a |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-04-13T04:18:52Z |
| publishDate | 2019-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-97b8c514c6bd480b8eab36003798445a2022-12-22T03:02:52ZengWileyMicrobial Biotechnology1751-79152019-07-0112466267610.1111/1751-7915.13399Bacterial nanocellulose production from naphthalenePatricia Marín0Sophie Marie Martirani‐Von Abercron1Leire Urbina2Daniel Pacheco‐Sánchez3Mayra Alejandra Castañeda‐Cataña4Aloña Retegi5Arantxa Eceiza6Silvia Marqués7Estación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Calle Profesor Albareda, 1 Granada 18008 SpainEstación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Calle Profesor Albareda, 1 Granada 18008 SpainMaterials + Technologies Research Group (GMT) Department of Chemical and Environmental Engineering Faculty of Engineering of Gipuzkoa University of the Basque Country Pza Europa 1 Donostia‐San Sebastian 20018 SpainEstación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Calle Profesor Albareda, 1 Granada 18008 SpainEstación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Calle Profesor Albareda, 1 Granada 18008 SpainMaterials + Technologies Research Group (GMT) Department of Chemical and Environmental Engineering Faculty of Engineering of Gipuzkoa University of the Basque Country Pza Europa 1 Donostia‐San Sebastian 20018 SpainMaterials + Technologies Research Group (GMT) Department of Chemical and Environmental Engineering Faculty of Engineering of Gipuzkoa University of the Basque Country Pza Europa 1 Donostia‐San Sebastian 20018 SpainEstación Experimental del Zaidín Department of Environmental Protection Consejo Superior de Investigaciones Científicas Calle Profesor Albareda, 1 Granada 18008 SpainSummary Polycyclic aromatic compounds (PAHs) are toxic compounds that are released in the environment as a consequence of industrial activities. The restoration of PAH‐polluted sites considers the use of bacteria capable of degrading aromatic compounds to carbon dioxide and water. Here we characterize a new Xanthobacteraceae strain, Starkeya sp. strain N1B, previously isolated during enrichment under microaerophilic conditions, which is capable of using naphthalene crystals as the sole carbon source. The strain produced a structured biofilm when grown on naphthalene crystals, which had the shape of a half‐sphere organized over the crystal. Scanning electron microscopy (SEM) and GC‐MS analysis indicated that the biofilm was essentially made of cellulose, composed of several micron‐long nanofibrils of 60 nm diameter. A cellulosic biofilm was also formed when the cells grew with glucose as the carbon source. Fourier transformed infrared spectroscopy (FTIR) confirmed that the polymer was type I cellulose in both cases, although the crystallinity of the material greatly depended on the carbon source used for growth. Using genome mining and mutant analysis, we identified the genetic complements required for the transformation of naphthalene into cellulose, which seemed to have been successively acquired through horizontal gene transfer. The capacity to develop the biofilm around the crystal was found to be dispensable for growth when naphthalene was used as the carbon source, suggesting that the function of this structure is more intricate than initially thought. This is the first example of the use of toxic aromatic hydrocarbons as the carbon source for bacterial cellulose production. Application of this capacity would allow the remediation of a PAH into such a value‐added polymer with multiple biotechnological usages.https://doi.org/10.1111/1751-7915.13399 |
| spellingShingle | Patricia Marín Sophie Marie Martirani‐Von Abercron Leire Urbina Daniel Pacheco‐Sánchez Mayra Alejandra Castañeda‐Cataña Aloña Retegi Arantxa Eceiza Silvia Marqués Bacterial nanocellulose production from naphthalene Microbial Biotechnology |
| title | Bacterial nanocellulose production from naphthalene |
| title_full | Bacterial nanocellulose production from naphthalene |
| title_fullStr | Bacterial nanocellulose production from naphthalene |
| title_full_unstemmed | Bacterial nanocellulose production from naphthalene |
| title_short | Bacterial nanocellulose production from naphthalene |
| title_sort | bacterial nanocellulose production from naphthalene |
| url | https://doi.org/10.1111/1751-7915.13399 |
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