Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves
Fluoroquinolone (FQ) resistance in a major foodborne bacterial pathogen, <i>Campylobacter jejuni</i>, derived from cattle has recently become prevalent and poses a significant public health concern. However, the underlying factors for this increase are not entirely clear. To evaluate the...
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MDPI AG
2022-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/10/1407 |
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| author | Debora Brito Goulart Ashenafi Feyisa Beyi Zuowei Wu Mehmet Cemal Adiguzel Samantha Wilson Changyun Xu Jinji Pang Renee Dewell Grant A. Dewell Paul J. Plummer Qijing Zhang Orhan Sahin |
| author_facet | Debora Brito Goulart Ashenafi Feyisa Beyi Zuowei Wu Mehmet Cemal Adiguzel Samantha Wilson Changyun Xu Jinji Pang Renee Dewell Grant A. Dewell Paul J. Plummer Qijing Zhang Orhan Sahin |
| author_sort | Debora Brito Goulart |
| collection | DOAJ |
| description | Fluoroquinolone (FQ) resistance in a major foodborne bacterial pathogen, <i>Campylobacter jejuni</i>, derived from cattle has recently become prevalent and poses a significant public health concern. However, the underlying factors for this increase are not entirely clear. To evaluate the effect of enrofloxacin treatment on FQ-resistance development in <i>C. jejuni</i>, 35 commercial calves were equally divided into five groups (Groups 1–5) and were orally inoculated with FQ-susceptible (FQ-S) <i>C. jejuni</i>. Eight days later, Groups 4 and 5 were challenged with <i>Mannheimia haemolytica</i> via a transtracheal route to induce a respiratory disease; after 8 days, Groups 2, 3, 4, and 5 were injected subcutaneously with enrofloxacin (7.5 mg/kg for Groups 2 and 4, and 12.5 mg/kg for Groups 3 and 5). Colonization levels by FQ-resistant (FQ-R) and FQ-S <i>Campylobacter</i> in rectal feces were determined via differential culture throughout the experiment. Before oral inoculation with <i>C. jejuni</i>, only five calves were naturally colonized by <i>Campylobacter</i>, four of which were also colonized by FQ-R <i>C. jejuni</i> (three in Group 1 and one in Group 3). Soon after the oral inoculation, almost all calves in the groups became stably colonized by FQ-S <i>C. jejuni</i> (~3–6 log<sub>10</sub> CFU/g), except that the four calves that were pre-colonized before inoculation remained positive with both FQ-R and FQ-S <i>C. jejuni</i>. Following enrofloxacin administration, <i>C. jejuni</i> colonization declined sharply and rapidly in all treated groups to undetectable levels; however, the vast majority of the animals were recolonized by <i>C. jejuni</i> at comparable levels 72 h after the treatment. Notably, no FQ-R <i>C. jejuni</i> was detected in any of the calves that received enrofloxacin, regardless of the drug dose used or disease status of the animals. The lack of detection of FQ-R <i>C. jejuni</i> was likely due to the localized high concentration of the antibiotic in the intestine, which may have prevented the emergence of the FQ-R mutant. These findings indicate that single-dose enrofloxacin use in cattle poses a low risk for selection of de novo FQ-R mutants in <i>C. jejuni</i>. |
| first_indexed | 2024-03-09T20:50:49Z |
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| language | English |
| last_indexed | 2024-03-09T20:50:49Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-97bbda0bcf6a463f9f6185358df750f52023-11-23T22:35:58ZengMDPI AGAntibiotics2079-63822022-10-011110140710.3390/antibiotics11101407Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in CalvesDebora Brito Goulart0Ashenafi Feyisa Beyi1Zuowei Wu2Mehmet Cemal Adiguzel3Samantha Wilson4Changyun Xu5Jinji Pang6Renee Dewell7Grant A. Dewell8Paul J. Plummer9Qijing Zhang10Orhan Sahin11Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USACenter for Food Security and Public Health, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USADepartment of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USAFluoroquinolone (FQ) resistance in a major foodborne bacterial pathogen, <i>Campylobacter jejuni</i>, derived from cattle has recently become prevalent and poses a significant public health concern. However, the underlying factors for this increase are not entirely clear. To evaluate the effect of enrofloxacin treatment on FQ-resistance development in <i>C. jejuni</i>, 35 commercial calves were equally divided into five groups (Groups 1–5) and were orally inoculated with FQ-susceptible (FQ-S) <i>C. jejuni</i>. Eight days later, Groups 4 and 5 were challenged with <i>Mannheimia haemolytica</i> via a transtracheal route to induce a respiratory disease; after 8 days, Groups 2, 3, 4, and 5 were injected subcutaneously with enrofloxacin (7.5 mg/kg for Groups 2 and 4, and 12.5 mg/kg for Groups 3 and 5). Colonization levels by FQ-resistant (FQ-R) and FQ-S <i>Campylobacter</i> in rectal feces were determined via differential culture throughout the experiment. Before oral inoculation with <i>C. jejuni</i>, only five calves were naturally colonized by <i>Campylobacter</i>, four of which were also colonized by FQ-R <i>C. jejuni</i> (three in Group 1 and one in Group 3). Soon after the oral inoculation, almost all calves in the groups became stably colonized by FQ-S <i>C. jejuni</i> (~3–6 log<sub>10</sub> CFU/g), except that the four calves that were pre-colonized before inoculation remained positive with both FQ-R and FQ-S <i>C. jejuni</i>. Following enrofloxacin administration, <i>C. jejuni</i> colonization declined sharply and rapidly in all treated groups to undetectable levels; however, the vast majority of the animals were recolonized by <i>C. jejuni</i> at comparable levels 72 h after the treatment. Notably, no FQ-R <i>C. jejuni</i> was detected in any of the calves that received enrofloxacin, regardless of the drug dose used or disease status of the animals. The lack of detection of FQ-R <i>C. jejuni</i> was likely due to the localized high concentration of the antibiotic in the intestine, which may have prevented the emergence of the FQ-R mutant. These findings indicate that single-dose enrofloxacin use in cattle poses a low risk for selection of de novo FQ-R mutants in <i>C. jejuni</i>.https://www.mdpi.com/2079-6382/11/10/1407<i>Campylobacter</i>antimicrobial resistancecattleintestinal colonizationfecesbovine respiratory disease (BRD) |
| spellingShingle | Debora Brito Goulart Ashenafi Feyisa Beyi Zuowei Wu Mehmet Cemal Adiguzel Samantha Wilson Changyun Xu Jinji Pang Renee Dewell Grant A. Dewell Paul J. Plummer Qijing Zhang Orhan Sahin Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves Antibiotics <i>Campylobacter</i> antimicrobial resistance cattle intestinal colonization feces bovine respiratory disease (BRD) |
| title | Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_full | Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_fullStr | Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_full_unstemmed | Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_short | Influence of Single Dose Enrofloxacin Injection on Development of Fluoroquinolone Resistance in <i>Campylobacter jejuni</i> in Calves |
| title_sort | influence of single dose enrofloxacin injection on development of fluoroquinolone resistance in i campylobacter jejuni i in calves |
| topic | <i>Campylobacter</i> antimicrobial resistance cattle intestinal colonization feces bovine respiratory disease (BRD) |
| url | https://www.mdpi.com/2079-6382/11/10/1407 |
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