Genome sequence data of the strongly antagonistic yeast Pichia kluyveri isolate APC 11.10 B as a foundation for analysing biocontrol mechanisms
Pichia kluyveri strain APC 11.10 B was isolated from apple bark in Switzerland and exhibited strong antagonistic activity against plant pathogenic fungi in vitro (e.g., Botrytis, Fusarium or Monilinia isolates). In order to identify the mechanisms underlying this antagonism, we have sequenced the ge...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340923005048 |
| _version_ | 1797744011856838656 |
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| author | Lukas Nägeli Martin Schuler Tina Segessemann Daniel Frei Jürg E. Frey Kenneth H. Wolfe Christian H. Ahrens Florian M. Freimoser |
| author_facet | Lukas Nägeli Martin Schuler Tina Segessemann Daniel Frei Jürg E. Frey Kenneth H. Wolfe Christian H. Ahrens Florian M. Freimoser |
| author_sort | Lukas Nägeli |
| collection | DOAJ |
| description | Pichia kluyveri strain APC 11.10 B was isolated from apple bark in Switzerland and exhibited strong antagonistic activity against plant pathogenic fungi in vitro (e.g., Botrytis, Fusarium or Monilinia isolates). In order to identify the mechanisms underlying this antagonism, we have sequenced the genome of this isolate by long- and short-read sequencing technologies. The sequence data were de novo assembled into nine scaffolds and a fully resolved circularized mitogenome. The total genome size was 10.9 Mbp and 7451 potential open reading frames (ORFs) and 202 tRNA genes were predicted. In comparison to two P. kluyveri genomes deposited at the NCBI (of strains X31-10 and CBA6002), the APC 11.10 B strain seemed to represent a hybrid because backmapping of sequencing reads resulted in a high rate of heterozygous and structural variants in the nuclear genome (this was not observed for the mitochondrial genome). The P. kluyveri (APC 11.10 B) draft genome represents a first step and resource for genome mining, comparative and functional genomics (e.g., identifying the biocontrol mode of action), and evolutionary studies. Since the genus Pichia comprises many biotechnologically relevant yeasts, the genome data may be used in a variety of fields and disciplines. |
| first_indexed | 2024-03-12T15:04:34Z |
| format | Article |
| id | doaj.art-97bccd1d32fa4e38a1c5cd730592dd26 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-03-12T15:04:34Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-97bccd1d32fa4e38a1c5cd730592dd262023-08-13T04:54:12ZengElsevierData in Brief2352-34092023-08-0149109394Genome sequence data of the strongly antagonistic yeast Pichia kluyveri isolate APC 11.10 B as a foundation for analysing biocontrol mechanismsLukas Nägeli0Martin Schuler1Tina Segessemann2Daniel Frei3Jürg E. Frey4Kenneth H. Wolfe5Christian H. Ahrens6Florian M. Freimoser7Agroscope, Research Division Plant Protection, Route de Duillier 60, 1260 Nyon 1, SwitzerlandAgroscope, Competence Division Method Development and Analytics, Reckenholzstrasse 190, 8046 Zürich, SwitzerlandAgroscope, Competence Division Method Development and Analytics, Reckenholzstrasse 190, 8046 Zürich, Switzerland; SIB, Swiss Institute of Bioinformatics, Reckenholzstrasse 190, 8046 Zürich, SwitzerlandAgroscope, Competence Division Method Development and Analytics, Reckenholzstrasse 190, 8046 Zürich, SwitzerlandAgroscope, Competence Division Method Development and Analytics, Reckenholzstrasse 190, 8046 Zürich, SwitzerlandConway Institute, University College Dublin, Dublin 4, IrelandAgroscope, Competence Division Method Development and Analytics, Reckenholzstrasse 190, 8046 Zürich, Switzerland; SIB, Swiss Institute of Bioinformatics, Reckenholzstrasse 190, 8046 Zürich, SwitzerlandAgroscope, Research Division Plant Protection, Route de Duillier 60, 1260 Nyon 1, Switzerland; Corresponding author.Pichia kluyveri strain APC 11.10 B was isolated from apple bark in Switzerland and exhibited strong antagonistic activity against plant pathogenic fungi in vitro (e.g., Botrytis, Fusarium or Monilinia isolates). In order to identify the mechanisms underlying this antagonism, we have sequenced the genome of this isolate by long- and short-read sequencing technologies. The sequence data were de novo assembled into nine scaffolds and a fully resolved circularized mitogenome. The total genome size was 10.9 Mbp and 7451 potential open reading frames (ORFs) and 202 tRNA genes were predicted. In comparison to two P. kluyveri genomes deposited at the NCBI (of strains X31-10 and CBA6002), the APC 11.10 B strain seemed to represent a hybrid because backmapping of sequencing reads resulted in a high rate of heterozygous and structural variants in the nuclear genome (this was not observed for the mitochondrial genome). The P. kluyveri (APC 11.10 B) draft genome represents a first step and resource for genome mining, comparative and functional genomics (e.g., identifying the biocontrol mode of action), and evolutionary studies. Since the genus Pichia comprises many biotechnologically relevant yeasts, the genome data may be used in a variety of fields and disciplines.http://www.sciencedirect.com/science/article/pii/S2352340923005048AntagonismBiocontrolGenome assembly and annotationMechanismPlant protectionYeast |
| spellingShingle | Lukas Nägeli Martin Schuler Tina Segessemann Daniel Frei Jürg E. Frey Kenneth H. Wolfe Christian H. Ahrens Florian M. Freimoser Genome sequence data of the strongly antagonistic yeast Pichia kluyveri isolate APC 11.10 B as a foundation for analysing biocontrol mechanisms Data in Brief Antagonism Biocontrol Genome assembly and annotation Mechanism Plant protection Yeast |
| title | Genome sequence data of the strongly antagonistic yeast Pichia kluyveri isolate APC 11.10 B as a foundation for analysing biocontrol mechanisms |
| title_full | Genome sequence data of the strongly antagonistic yeast Pichia kluyveri isolate APC 11.10 B as a foundation for analysing biocontrol mechanisms |
| title_fullStr | Genome sequence data of the strongly antagonistic yeast Pichia kluyveri isolate APC 11.10 B as a foundation for analysing biocontrol mechanisms |
| title_full_unstemmed | Genome sequence data of the strongly antagonistic yeast Pichia kluyveri isolate APC 11.10 B as a foundation for analysing biocontrol mechanisms |
| title_short | Genome sequence data of the strongly antagonistic yeast Pichia kluyveri isolate APC 11.10 B as a foundation for analysing biocontrol mechanisms |
| title_sort | genome sequence data of the strongly antagonistic yeast pichia kluyveri isolate apc 11 10 b as a foundation for analysing biocontrol mechanisms |
| topic | Antagonism Biocontrol Genome assembly and annotation Mechanism Plant protection Yeast |
| url | http://www.sciencedirect.com/science/article/pii/S2352340923005048 |
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