STUDY THE EFFECTS OF (8-OXO-GUANINE DNA GLYCOSYLASE 1 RS1052133) POLYMORPHISM ON GENE EXPRESSION AS A PREDISPOSING FACTOR FOR DNA MUTATION IN MIDLAND REFINERIES COMPANY-DURA REFINERY WORKERS
Background: Refineries are exposed to different types of air pollutants, among them, polycyclic aromatic hydrocarbons (PAHs), they are a group of over hundred different kinds of hazardous organic chemicals (pollutants), which are formed primarily during the incomplete burning of oil, gas an...
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| Format: | Article |
| Language: | English |
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Al-Nahrain University/College of medicine
2022-06-01
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| Series: | The Iraqi Journal of Medical Sciences |
| Online Access: | https://www.iraqijms.net/index.php?do=view&type=article&id=819 |
| _version_ | 1817978703783657472 |
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| author | Raghad H. Al-Ani Estabraq A. Al-Wasiti Ahmed S. Shukur |
| author_facet | Raghad H. Al-Ani Estabraq A. Al-Wasiti Ahmed S. Shukur |
| author_sort | Raghad H. Al-Ani |
| collection | DOAJ |
| description | Background: Refineries are exposed to different types of air pollutants, among them, polycyclic aromatic hydrocarbons (PAHs), they are a group of over hundred different kinds of hazardous organic chemicals (pollutants), which are formed primarily during the incomplete burning of oil, gas and coal. PAHs are metabolized in different organs of human body especially in the liver leading to generation of mutagenic metabolites, of them, the reactive oxygen species, which damage DNA by binding with guanine base leading to formation of 8-hydroxyguanine. The 8-oxo-guanine DNA glycosylase (hOGG1 rs1052133) gene, is a regulatory gene binds with 8-oxo-DG in the DNA to initiate base excision repair (BER) pathway. Objective: To investigate the role of (hOGG1 rs1052133) gene polymorphism in single nucleotide polymorphism repair. Methods: One hundred sixty-eight (168) subjects were participated in this study. They were they divided into three groups (country side, office workers and field workers). PAHs were analyzed in all participant by Gas Chromatography Coupled with a Mass Spectrophotometry GC/MS. SNP for hOGG1 and gene expression were detecting by real time polymerase chain reaction and quantitative polymerase chain reaction for each subject respectively. Results: PAH were detected in blood serum of refineries workers and not detected in country side participants. The level of hOGG1 gene expression was higher in GG genotype than GC and CC genotype. Conclusion: Refineries worker are at high risk of developing malignant transformation and serious disease due to high level of PAH compared to other population. hOGG1 plays an important role in DNA repair following DNA mutation. Keywords: Poly cyclic aromatic hydrocarbons, HOGGI, gene expression Citation: Al-Ani RH, Al-Wasiti EA, Shukur AS. Study the Effects of (8-oxo-Guanine DNA Glycosylase 1 rs1052133) Polymorphism on Gene Expression as a Predisposing Factor for DNA Mutation in Midland Refineries Company-Dura Refinery Workers. Iraqi JMS. 2022; 20(1): 90-97. doi: 10.22578/IJMS.20.1.12 |
| first_indexed | 2024-04-13T22:33:37Z |
| format | Article |
| id | doaj.art-97be34fca6a14180a0633b1ad16c3b98 |
| institution | Directory Open Access Journal |
| issn | 1681-6579 2224-4719 |
| language | English |
| last_indexed | 2024-04-13T22:33:37Z |
| publishDate | 2022-06-01 |
| publisher | Al-Nahrain University/College of medicine |
| record_format | Article |
| series | The Iraqi Journal of Medical Sciences |
| spelling | doaj.art-97be34fca6a14180a0633b1ad16c3b982022-12-22T02:26:51ZengAl-Nahrain University/College of medicineThe Iraqi Journal of Medical Sciences1681-65792224-47192022-06-01201909710.22578/IJMS.20.1.12STUDY THE EFFECTS OF (8-OXO-GUANINE DNA GLYCOSYLASE 1 RS1052133) POLYMORPHISM ON GENE EXPRESSION AS A PREDISPOSING FACTOR FOR DNA MUTATION IN MIDLAND REFINERIES COMPANY-DURA REFINERY WORKERSRaghad H. Al-AniEstabraq A. Al-WasitiAhmed S. Shukur Background: Refineries are exposed to different types of air pollutants, among them, polycyclic aromatic hydrocarbons (PAHs), they are a group of over hundred different kinds of hazardous organic chemicals (pollutants), which are formed primarily during the incomplete burning of oil, gas and coal. PAHs are metabolized in different organs of human body especially in the liver leading to generation of mutagenic metabolites, of them, the reactive oxygen species, which damage DNA by binding with guanine base leading to formation of 8-hydroxyguanine. The 8-oxo-guanine DNA glycosylase (hOGG1 rs1052133) gene, is a regulatory gene binds with 8-oxo-DG in the DNA to initiate base excision repair (BER) pathway. Objective: To investigate the role of (hOGG1 rs1052133) gene polymorphism in single nucleotide polymorphism repair. Methods: One hundred sixty-eight (168) subjects were participated in this study. They were they divided into three groups (country side, office workers and field workers). PAHs were analyzed in all participant by Gas Chromatography Coupled with a Mass Spectrophotometry GC/MS. SNP for hOGG1 and gene expression were detecting by real time polymerase chain reaction and quantitative polymerase chain reaction for each subject respectively. Results: PAH were detected in blood serum of refineries workers and not detected in country side participants. The level of hOGG1 gene expression was higher in GG genotype than GC and CC genotype. Conclusion: Refineries worker are at high risk of developing malignant transformation and serious disease due to high level of PAH compared to other population. hOGG1 plays an important role in DNA repair following DNA mutation. Keywords: Poly cyclic aromatic hydrocarbons, HOGGI, gene expression Citation: Al-Ani RH, Al-Wasiti EA, Shukur AS. Study the Effects of (8-oxo-Guanine DNA Glycosylase 1 rs1052133) Polymorphism on Gene Expression as a Predisposing Factor for DNA Mutation in Midland Refineries Company-Dura Refinery Workers. Iraqi JMS. 2022; 20(1): 90-97. doi: 10.22578/IJMS.20.1.12https://www.iraqijms.net/index.php?do=view&type=article&id=819 |
| spellingShingle | Raghad H. Al-Ani Estabraq A. Al-Wasiti Ahmed S. Shukur STUDY THE EFFECTS OF (8-OXO-GUANINE DNA GLYCOSYLASE 1 RS1052133) POLYMORPHISM ON GENE EXPRESSION AS A PREDISPOSING FACTOR FOR DNA MUTATION IN MIDLAND REFINERIES COMPANY-DURA REFINERY WORKERS The Iraqi Journal of Medical Sciences |
| title | STUDY THE EFFECTS OF (8-OXO-GUANINE DNA GLYCOSYLASE 1 RS1052133) POLYMORPHISM ON GENE EXPRESSION AS A PREDISPOSING FACTOR FOR DNA MUTATION IN MIDLAND REFINERIES COMPANY-DURA REFINERY WORKERS |
| title_full | STUDY THE EFFECTS OF (8-OXO-GUANINE DNA GLYCOSYLASE 1 RS1052133) POLYMORPHISM ON GENE EXPRESSION AS A PREDISPOSING FACTOR FOR DNA MUTATION IN MIDLAND REFINERIES COMPANY-DURA REFINERY WORKERS |
| title_fullStr | STUDY THE EFFECTS OF (8-OXO-GUANINE DNA GLYCOSYLASE 1 RS1052133) POLYMORPHISM ON GENE EXPRESSION AS A PREDISPOSING FACTOR FOR DNA MUTATION IN MIDLAND REFINERIES COMPANY-DURA REFINERY WORKERS |
| title_full_unstemmed | STUDY THE EFFECTS OF (8-OXO-GUANINE DNA GLYCOSYLASE 1 RS1052133) POLYMORPHISM ON GENE EXPRESSION AS A PREDISPOSING FACTOR FOR DNA MUTATION IN MIDLAND REFINERIES COMPANY-DURA REFINERY WORKERS |
| title_short | STUDY THE EFFECTS OF (8-OXO-GUANINE DNA GLYCOSYLASE 1 RS1052133) POLYMORPHISM ON GENE EXPRESSION AS A PREDISPOSING FACTOR FOR DNA MUTATION IN MIDLAND REFINERIES COMPANY-DURA REFINERY WORKERS |
| title_sort | study the effects of 8 oxo guanine dna glycosylase 1 rs1052133 polymorphism on gene expression as a predisposing factor for dna mutation in midland refineries company dura refinery workers |
| url | https://www.iraqijms.net/index.php?do=view&type=article&id=819 |
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