Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide of <i>Escherichia coli</i> O157:H7
<i>Escherichia coli</i> O157:H7 is a global concern that causes serious diseases, such as hemolytic uremic syndrome and bloody diarrhea. To control <i>E. coli</i> O157:H7 in food, a novel siphophage, BECP10, that targets the O157 serotype was isolated and characterized. Unlik...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/8/1469 |
| _version_ | 1797521881116442624 |
|---|---|
| author | Do-Won Park Jong-Hyun Park |
| author_facet | Do-Won Park Jong-Hyun Park |
| author_sort | Do-Won Park |
| collection | DOAJ |
| description | <i>Escherichia coli</i> O157:H7 is a global concern that causes serious diseases, such as hemolytic uremic syndrome and bloody diarrhea. To control <i>E. coli</i> O157:H7 in food, a novel siphophage, BECP10, that targets the O157 serotype was isolated and characterized. Unlike other <i>E. coli</i> phages, BECP10 can only infect <i>E. coli</i> O157 strains, and thus, did not infect other strains. The 48 kbp genome of BECP10 contained 76 open reading frames (ORFs), including 33 putative functional ORFs. The phage did not contain lysogeny-related modules or toxin-associated genes, suggesting that the phage might be strictly lytic. The tail spike protein (TSP) sequence had very low homology with the reported T1-like phages, indicating that TSP might be related to this unique host spectrum. The specific O-antigen residue of <i>E. coli</i> O157:H7 may be a key factor for phage infection by adsorption and receptor identification. The phage exhibited strong antibacterial activity against <i>E. coli</i> O157:H7 over a broad pH range and showed little development of phage-insensitive mutants. The phage sustained viability on the burger patties and reduced <i>E. coli</i> O157:H7 to a non-detectable level without the emergence of resistant cells at low temperatures for five days. Therefore, phage BECP10 might be a good biocontrol agent for <i>E. coli</i> O157:H7-contaminated food matrices. |
| first_indexed | 2024-03-10T08:18:46Z |
| format | Article |
| id | doaj.art-97becbbc28bd41f1824927558e5e9928 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T08:18:46Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-97becbbc28bd41f1824927558e5e99282023-11-22T10:09:45ZengMDPI AGViruses1999-49152021-07-01138146910.3390/v13081469Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide of <i>Escherichia coli</i> O157:H7Do-Won Park0Jong-Hyun Park1Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, KoreaDepartment of Food Science and Biotechnology, Gachon University, Seongnam 13120, Korea<i>Escherichia coli</i> O157:H7 is a global concern that causes serious diseases, such as hemolytic uremic syndrome and bloody diarrhea. To control <i>E. coli</i> O157:H7 in food, a novel siphophage, BECP10, that targets the O157 serotype was isolated and characterized. Unlike other <i>E. coli</i> phages, BECP10 can only infect <i>E. coli</i> O157 strains, and thus, did not infect other strains. The 48 kbp genome of BECP10 contained 76 open reading frames (ORFs), including 33 putative functional ORFs. The phage did not contain lysogeny-related modules or toxin-associated genes, suggesting that the phage might be strictly lytic. The tail spike protein (TSP) sequence had very low homology with the reported T1-like phages, indicating that TSP might be related to this unique host spectrum. The specific O-antigen residue of <i>E. coli</i> O157:H7 may be a key factor for phage infection by adsorption and receptor identification. The phage exhibited strong antibacterial activity against <i>E. coli</i> O157:H7 over a broad pH range and showed little development of phage-insensitive mutants. The phage sustained viability on the burger patties and reduced <i>E. coli</i> O157:H7 to a non-detectable level without the emergence of resistant cells at low temperatures for five days. Therefore, phage BECP10 might be a good biocontrol agent for <i>E. coli</i> O157:H7-contaminated food matrices.https://www.mdpi.com/1999-4915/13/8/1469<i>E. coli</i> O157:H7bacteriophagephage receptorantibacterial activityfood application |
| spellingShingle | Do-Won Park Jong-Hyun Park Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide of <i>Escherichia coli</i> O157:H7 Viruses <i>E. coli</i> O157:H7 bacteriophage phage receptor antibacterial activity food application |
| title | Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide of <i>Escherichia coli</i> O157:H7 |
| title_full | Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide of <i>Escherichia coli</i> O157:H7 |
| title_fullStr | Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide of <i>Escherichia coli</i> O157:H7 |
| title_full_unstemmed | Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide of <i>Escherichia coli</i> O157:H7 |
| title_short | Characterization and Food Application of the Novel Lytic Phage BECP10: Specifically Recognizes the O-polysaccharide of <i>Escherichia coli</i> O157:H7 |
| title_sort | characterization and food application of the novel lytic phage becp10 specifically recognizes the o polysaccharide of i escherichia coli i o157 h7 |
| topic | <i>E. coli</i> O157:H7 bacteriophage phage receptor antibacterial activity food application |
| url | https://www.mdpi.com/1999-4915/13/8/1469 |
| work_keys_str_mv | AT dowonpark characterizationandfoodapplicationofthenovellyticphagebecp10specificallyrecognizestheopolysaccharideofiescherichiacoliio157h7 AT jonghyunpark characterizationandfoodapplicationofthenovellyticphagebecp10specificallyrecognizestheopolysaccharideofiescherichiacoliio157h7 |