Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, Iran
Background: We aimed at genotyping and evaluating the predominance of G. duodenalis assemblages isolated from patients referred to medical laboratories in Khorramabad, Iran from Nov 2015 to Sep 2016. Hence, the development of a cost-effective HRM approach to determine genotypes of G. duodenalis base...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-06-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2941 |
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| author | Akram SEPAHVAND Ahmad HOSSEINI-SAFA Hossein Ali YOUSOFI Mohammad Hassan TAJEDINI Reza PAHLAVAN GHAREHBABAH Nader PESTEHCHIAN |
| author_facet | Akram SEPAHVAND Ahmad HOSSEINI-SAFA Hossein Ali YOUSOFI Mohammad Hassan TAJEDINI Reza PAHLAVAN GHAREHBABAH Nader PESTEHCHIAN |
| author_sort | Akram SEPAHVAND |
| collection | DOAJ |
| description | Background: We aimed at genotyping and evaluating the predominance of G. duodenalis assemblages isolated from patients referred to medical laboratories in Khorramabad, Iran from Nov 2015 to Sep 2016. Hence, the development of a cost-effective HRM approach to determine genotypes of G. duodenalis based on the triosephosphate isomerase (tpi) gene was examined and the genotyping results with and without diarrhea was compared.
Methods: Seventy G. duodenalis positive fecal samples were collected. A microscopic confirmation for the presence of Giardia spp. was performed, cysts of 70 Giardia spp. positive specimens were concentrated using sucrose flotation technique and sucrose solution PCR amplification was performed on 69 of 70 (98.5%) samples, and High Resolution Melting (HRM) analysis was performed using a software.
Results: The results showed two distinct genotypes (assemblages A and B) of G. duodenalis but infections with mixture of both assemblages were not detected. The genotypes of G. duodenalis showed that the sub assemblage AI, BIII and BIV were present in a proportion of 68.1%, 20.3% and 11.6% respectively in samples. Assemblage AI was significantly (P<0.05) more frequently found in patients with diarrhea.
Conclusion: The sub-assemblage AI, BIII, and BIV are more zoonotic potential. According to the comparison of the results of this study with the results of previous studies in this area and around of it, as well as the way people live and keep pets. This pattern established in Khorramabad city. HRM can be an ideal technique to detect and genotyping of G. duodenalis in clinical samples. |
| first_indexed | 2024-12-23T14:49:48Z |
| format | Article |
| id | doaj.art-97bef2eb869d41768bb4dba7505e57b5 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-23T14:49:48Z |
| publishDate | 2020-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-97bef2eb869d41768bb4dba7505e57b52022-12-21T17:43:00ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2020-06-0115210.18502/ijpa.v15i2.33022941Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, IranAkram SEPAHVAND0Ahmad HOSSEINI-SAFA1Hossein Ali YOUSOFI2Mohammad Hassan TAJEDINI3Reza PAHLAVAN GHAREHBABAH4Nader PESTEHCHIAN5Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran AND Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, IranInfectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran AND Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranApplied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, IranDepartment of Medical Biotechnology, Faculty of Advance Medical Science, Tabriz University of Medical Sciences, Tabriz, IranInfectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran AND Department of Medical Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranBackground: We aimed at genotyping and evaluating the predominance of G. duodenalis assemblages isolated from patients referred to medical laboratories in Khorramabad, Iran from Nov 2015 to Sep 2016. Hence, the development of a cost-effective HRM approach to determine genotypes of G. duodenalis based on the triosephosphate isomerase (tpi) gene was examined and the genotyping results with and without diarrhea was compared. Methods: Seventy G. duodenalis positive fecal samples were collected. A microscopic confirmation for the presence of Giardia spp. was performed, cysts of 70 Giardia spp. positive specimens were concentrated using sucrose flotation technique and sucrose solution PCR amplification was performed on 69 of 70 (98.5%) samples, and High Resolution Melting (HRM) analysis was performed using a software. Results: The results showed two distinct genotypes (assemblages A and B) of G. duodenalis but infections with mixture of both assemblages were not detected. The genotypes of G. duodenalis showed that the sub assemblage AI, BIII and BIV were present in a proportion of 68.1%, 20.3% and 11.6% respectively in samples. Assemblage AI was significantly (P<0.05) more frequently found in patients with diarrhea. Conclusion: The sub-assemblage AI, BIII, and BIV are more zoonotic potential. According to the comparison of the results of this study with the results of previous studies in this area and around of it, as well as the way people live and keep pets. This pattern established in Khorramabad city. HRM can be an ideal technique to detect and genotyping of G. duodenalis in clinical samples.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2941Giardia duodenalisGenotypingTriosephosphate isomer-ase geneHigh resolution melting; Iran |
| spellingShingle | Akram SEPAHVAND Ahmad HOSSEINI-SAFA Hossein Ali YOUSOFI Mohammad Hassan TAJEDINI Reza PAHLAVAN GHAREHBABAH Nader PESTEHCHIAN Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, Iran Iranian Journal of Parasitology Giardia duodenalis Genotyping Triosephosphate isomer-ase gene High resolution melting; Iran |
| title | Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, Iran |
| title_full | Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, Iran |
| title_fullStr | Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, Iran |
| title_full_unstemmed | Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, Iran |
| title_short | Genotype Characteristics of Giardia duodenalis in Patients Using High Resolution Melting Analysis Technique in Khorramabad, Iran |
| title_sort | genotype characteristics of giardia duodenalis in patients using high resolution melting analysis technique in khorramabad iran |
| topic | Giardia duodenalis Genotyping Triosephosphate isomer-ase gene High resolution melting; Iran |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2941 |
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