Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seeds
<p>Abstract</p> <p>Background</p> <p>The edible endosperm of <it>Lodoicea maldivica </it>with the common name of <it>coco de mer </it>is used in Chinese medicine for treating cough. Native to Seychelles, <it>Lodoicea maldivica </it>se...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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BMC
2011-09-01
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| Series: | Chinese Medicine |
| Online Access: | http://www.cmjournal.org/content/6/1/34 |
| _version_ | 1811283131180777472 |
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| author | Mok Chuen-shing Mak Chun-yin |
| author_facet | Mok Chuen-shing Mak Chun-yin |
| author_sort | Mok Chuen-shing |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The edible endosperm of <it>Lodoicea maldivica </it>with the common name of <it>coco de mer </it>is used in Chinese medicine for treating cough. Native to Seychelles, <it>Lodoicea maldivica </it>seeds have commanded high prices for centuries due to its scarcity. This study aims to develop a molecular identification method for the authentication of <it>Lodoicea maldivica </it>seeds.</p> <p>Methods</p> <p>DNA was extracted from the sample. Two polymerase chain reaction (PCR) systems were developed to amplify a region of the chloroplast DNA and the nuclear phosphoribulokinase (PRK) region specific to <it>Lodoicea maldivica </it>respectively. DNA sequence of a sample was determined and compared with that of the <it>Lodoicea maldivica </it>reference material.</p> <p>Results</p> <p>The PRK gene of <it>Lodoicea maldivica </it>was successfully amplified and sequenced for identification.</p> <p>Conclusion</p> <p>A new molecular method for the identification of <it>Lodoicea maldivica </it>seeds in fresh, frozen or dried forms was developed.</p> |
| first_indexed | 2024-04-13T02:06:15Z |
| format | Article |
| id | doaj.art-97c0880740e149bea24758487b43f34b |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-04-13T02:06:15Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-97c0880740e149bea24758487b43f34b2022-12-22T03:07:27ZengBMCChinese Medicine1749-85462011-09-01613410.1186/1749-8546-6-34Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seedsMok Chuen-shingMak Chun-yin<p>Abstract</p> <p>Background</p> <p>The edible endosperm of <it>Lodoicea maldivica </it>with the common name of <it>coco de mer </it>is used in Chinese medicine for treating cough. Native to Seychelles, <it>Lodoicea maldivica </it>seeds have commanded high prices for centuries due to its scarcity. This study aims to develop a molecular identification method for the authentication of <it>Lodoicea maldivica </it>seeds.</p> <p>Methods</p> <p>DNA was extracted from the sample. Two polymerase chain reaction (PCR) systems were developed to amplify a region of the chloroplast DNA and the nuclear phosphoribulokinase (PRK) region specific to <it>Lodoicea maldivica </it>respectively. DNA sequence of a sample was determined and compared with that of the <it>Lodoicea maldivica </it>reference material.</p> <p>Results</p> <p>The PRK gene of <it>Lodoicea maldivica </it>was successfully amplified and sequenced for identification.</p> <p>Conclusion</p> <p>A new molecular method for the identification of <it>Lodoicea maldivica </it>seeds in fresh, frozen or dried forms was developed.</p>http://www.cmjournal.org/content/6/1/34 |
| spellingShingle | Mok Chuen-shing Mak Chun-yin Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seeds Chinese Medicine |
| title | Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seeds |
| title_full | Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seeds |
| title_fullStr | Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seeds |
| title_full_unstemmed | Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seeds |
| title_short | Molecular identification of <it>Lodoicea maldivica </it>(<it>coco de mer</it>) seeds |
| title_sort | molecular identification of it lodoicea maldivica it it coco de mer it seeds |
| url | http://www.cmjournal.org/content/6/1/34 |
| work_keys_str_mv | AT mokchuenshing molecularidentificationofitlodoiceamaldivicaititcocodemeritseeds AT makchunyin molecularidentificationofitlodoiceamaldivicaititcocodemeritseeds |