Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius
Abstract Background As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isol...
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02993-9 |
| _version_ | 1797453699499425792 |
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| author | Jiajun Yang Peng Shang Bo Zhang Jing Wang Zhenyu Du Shanfeng Wang Jun Xing Hao Zhang |
| author_facet | Jiajun Yang Peng Shang Bo Zhang Jing Wang Zhenyu Du Shanfeng Wang Jun Xing Hao Zhang |
| author_sort | Jiajun Yang |
| collection | DOAJ |
| description | Abstract Background As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and shown to colonize the ileal mucous membrane, thereby promoting nutritional digestion, absorption, and immunity. To evaluate its probiotic role, the entire genome was sequenced, the genetic information was annotated, and the metabolic information was analyzed. Results The phylogenetic relationship indicated that the bacteria was closer to L. salivarius MT573555.1 and MT585431.1. Functional genes included transporters, membrane proteins, enzymes, heavy metal resistance proteins, and putative proteins; metabolism-related genes were the most abundant. The six types of metabolic pathways secreted by L. salivarius were mainly composed of secretory transmembrane proteins and peptides. The secretory proteins of L. salivarius were digestive enzymes, functional proteins that regulate apoptosis, antibodies, and hormones. Non-targeted metabolomic analysis of L. salivarius metabolites suggested that ceramide, pyrrolidone- 5- carboxylic acid, N2-acetyl-L-ornithine, 2-ethyl-2-hydroxybutyric acid, N-lactoyl-phenylalanine, and 12 others were involved in antioxidation, repair of the cellular membrane, anticonvulsant, hypnosis, and appetite inhibition. Metabolites of clavaminic acid, antibiotic X14889C, and five other types of bacteriocins were identified, namely phenyllactic acid, janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin. The adherence and antioxidation of L. salivarius were also predicted. No virulence genes were found. Conclusion The main probiotic properties of L. salivarius were identified using genomic, metabonomic, and biochemical assays, which are beneficial for porcine feeding. Our results provided deeper insights into the probiotic effects of L. salivarius. |
| first_indexed | 2024-03-09T15:26:28Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-09T15:26:28Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-97c1840db6ae49ae9ee15c0fe5ecde012023-11-26T12:30:05ZengBMCBMC Microbiology1471-21802023-08-0123111310.1186/s12866-023-02993-9Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivariusJiajun Yang0Peng Shang1Bo Zhang2Jing Wang3Zhenyu Du4Shanfeng Wang5Jun Xing6Hao Zhang7School of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and ForestryCollege of Animal Science, Tibet Agriculture and Animal Husbandry CollegeBeijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversitySchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and ForestrySchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and ForestrySchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and ForestrySchool of Animal Husbandry and Veterinary Medicine, Jiangsu Vocational College of Agriculture and ForestryBeijing Key Laboratory for Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural UniversityAbstract Background As substitutes for antibiotics, probiotic bacteria protect against digestive infections caused by pathogenic bacteria. Ligilactobacillus salivarius is a species of native lactobacillus found in both humans and animals. Herein, a swine-derived Ligilactobacillus salivarius was isolated and shown to colonize the ileal mucous membrane, thereby promoting nutritional digestion, absorption, and immunity. To evaluate its probiotic role, the entire genome was sequenced, the genetic information was annotated, and the metabolic information was analyzed. Results The phylogenetic relationship indicated that the bacteria was closer to L. salivarius MT573555.1 and MT585431.1. Functional genes included transporters, membrane proteins, enzymes, heavy metal resistance proteins, and putative proteins; metabolism-related genes were the most abundant. The six types of metabolic pathways secreted by L. salivarius were mainly composed of secretory transmembrane proteins and peptides. The secretory proteins of L. salivarius were digestive enzymes, functional proteins that regulate apoptosis, antibodies, and hormones. Non-targeted metabolomic analysis of L. salivarius metabolites suggested that ceramide, pyrrolidone- 5- carboxylic acid, N2-acetyl-L-ornithine, 2-ethyl-2-hydroxybutyric acid, N-lactoyl-phenylalanine, and 12 others were involved in antioxidation, repair of the cellular membrane, anticonvulsant, hypnosis, and appetite inhibition. Metabolites of clavaminic acid, antibiotic X14889C, and five other types of bacteriocins were identified, namely phenyllactic acid, janthitrem G, 13-demethyl tacrolimus, medinoside E, and tertonasin. The adherence and antioxidation of L. salivarius were also predicted. No virulence genes were found. Conclusion The main probiotic properties of L. salivarius were identified using genomic, metabonomic, and biochemical assays, which are beneficial for porcine feeding. Our results provided deeper insights into the probiotic effects of L. salivarius.https://doi.org/10.1186/s12866-023-02993-9Ligilactobacillus salivariusProbioticMetabonomic analysisMetagenomic analysis |
| spellingShingle | Jiajun Yang Peng Shang Bo Zhang Jing Wang Zhenyu Du Shanfeng Wang Jun Xing Hao Zhang Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius BMC Microbiology Ligilactobacillus salivarius Probiotic Metabonomic analysis Metagenomic analysis |
| title | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
| title_full | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
| title_fullStr | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
| title_full_unstemmed | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
| title_short | Genomic and metabonomic methods reveal the probiotic functions of swine-derived Ligilactobacillus salivarius |
| title_sort | genomic and metabonomic methods reveal the probiotic functions of swine derived ligilactobacillus salivarius |
| topic | Ligilactobacillus salivarius Probiotic Metabonomic analysis Metagenomic analysis |
| url | https://doi.org/10.1186/s12866-023-02993-9 |
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