Time and temperature stability of Tritrichomonas foetus in phosphate-buffered saline as evaluated by a reverse transcription real-time PCR assay and field analysis

Time and temperature stability of Tritrichomonas foetus in phosphate-buffered saline as evaluated by a reverse transcription real-time PCR assay and field analysis

Tritrichomonas foetus (TF) is a significant reproductive pathogen of cattle, and sample collection, handling, transport, and testing are significant hurdles to surveillance programs. Recent methods have been developed that allow for the direct detection of TF using a reverse transcription real-time...

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Bibliographic Details
Main Authors: Duan S. Loy, Renata Spuri Gomes, Enakshy Dutta, Bruce W. Brodersen, John Dustin Loy
Format: Article
Language:English
Published: Frontiers Media S.A. 2023-03-01
Series:Frontiers in Veterinary Science
Subjects:
time
temperature
RNA stability
PBS
Tritrichomonas foetus
direct reverse transcription quantitative real-time PCR
Online Access:https://www.frontiersin.org/articles/10.3389/fvets.2023.1101502/full

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