Characterization of Bacterial and Fungal Biofilms in Chronic Rhinosinusitis
Background Conclusive evidence exists that biofilms are present on the mucosa of chronic rhinosinusitis (CRS) patients. Less is known about the species constituting these biofilms. This study developed a fluorescence in situ hybridization (FISH) protocol for characterization of bacterial and fungal...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2010-01-01
|
| Series: | Allergy & Rhinology |
| Online Access: | https://doi.org/10.2500/ajra.2009.23.3413a |
| _version_ | 1818162132993179648 |
|---|---|
| author | Andrew Foreman B.M.B.S. (Hons.) Alkis James Psaltis Ph.D., M.B.B.S. Lor Wai Tan Ph.D. Peter-John Wormald M.D. |
| author_facet | Andrew Foreman B.M.B.S. (Hons.) Alkis James Psaltis Ph.D., M.B.B.S. Lor Wai Tan Ph.D. Peter-John Wormald M.D. |
| author_sort | Andrew Foreman B.M.B.S. (Hons.) |
| collection | DOAJ |
| description | Background Conclusive evidence exists that biofilms are present on the mucosa of chronic rhinosinusitis (CRS) patients. Less is known about the species constituting these biofilms. This study developed a fluorescence in situ hybridization (FISH) protocol for characterization of bacterial and fungal biofilms in CRS. Methods Fifty CRS patients and 10 controls were recruited. Bacteria FISH probes for Staphylococcus aureus, Haemophilus influenzae/ and Pseudomonas aeruginosa and a universal probe for fungi were applied to sinus mucosal specimens and then analyzed using confocal scanning laser microscopy. Results Thirty-six of 50 CRS patients had biofilms present in contrast to 0/10 controls, suggesting a role for biofilms in the pathogenesis of this disease. S. aureus was the most common biofilm-forming organism. Eleven of 50 CRS patients hid characteristic fungal biofilms present. Conclusion This is the largest study of biofilms in CRS. It has validated mucosal tissue cryopreservation for delayed biofilm analysis. Fungal biofilms have been identified and the importance of S. aureus biofilms in the polymicrobial etiology of CRS is highlighted. |
| first_indexed | 2024-12-11T16:28:49Z |
| format | Article |
| id | doaj.art-97c83b98dc7649fd9ae6068f88ed5e3c |
| institution | Directory Open Access Journal |
| issn | 2152-6567 |
| language | English |
| last_indexed | 2024-12-11T16:28:49Z |
| publishDate | 2010-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Allergy & Rhinology |
| spelling | doaj.art-97c83b98dc7649fd9ae6068f88ed5e3c2022-12-22T00:58:38ZengSAGE PublishingAllergy & Rhinology2152-65672010-01-01110.2500/ajra.2009.23.3413aCharacterization of Bacterial and Fungal Biofilms in Chronic RhinosinusitisAndrew Foreman B.M.B.S. (Hons.)Alkis James Psaltis Ph.D., M.B.B.S.Lor Wai Tan Ph.D.Peter-John Wormald M.D.Background Conclusive evidence exists that biofilms are present on the mucosa of chronic rhinosinusitis (CRS) patients. Less is known about the species constituting these biofilms. This study developed a fluorescence in situ hybridization (FISH) protocol for characterization of bacterial and fungal biofilms in CRS. Methods Fifty CRS patients and 10 controls were recruited. Bacteria FISH probes for Staphylococcus aureus, Haemophilus influenzae/ and Pseudomonas aeruginosa and a universal probe for fungi were applied to sinus mucosal specimens and then analyzed using confocal scanning laser microscopy. Results Thirty-six of 50 CRS patients had biofilms present in contrast to 0/10 controls, suggesting a role for biofilms in the pathogenesis of this disease. S. aureus was the most common biofilm-forming organism. Eleven of 50 CRS patients hid characteristic fungal biofilms present. Conclusion This is the largest study of biofilms in CRS. It has validated mucosal tissue cryopreservation for delayed biofilm analysis. Fungal biofilms have been identified and the importance of S. aureus biofilms in the polymicrobial etiology of CRS is highlighted.https://doi.org/10.2500/ajra.2009.23.3413a |
| spellingShingle | Andrew Foreman B.M.B.S. (Hons.) Alkis James Psaltis Ph.D., M.B.B.S. Lor Wai Tan Ph.D. Peter-John Wormald M.D. Characterization of Bacterial and Fungal Biofilms in Chronic Rhinosinusitis Allergy & Rhinology |
| title | Characterization of Bacterial and Fungal Biofilms in Chronic Rhinosinusitis |
| title_full | Characterization of Bacterial and Fungal Biofilms in Chronic Rhinosinusitis |
| title_fullStr | Characterization of Bacterial and Fungal Biofilms in Chronic Rhinosinusitis |
| title_full_unstemmed | Characterization of Bacterial and Fungal Biofilms in Chronic Rhinosinusitis |
| title_short | Characterization of Bacterial and Fungal Biofilms in Chronic Rhinosinusitis |
| title_sort | characterization of bacterial and fungal biofilms in chronic rhinosinusitis |
| url | https://doi.org/10.2500/ajra.2009.23.3413a |
| work_keys_str_mv | AT andrewforemanbmbshons characterizationofbacterialandfungalbiofilmsinchronicrhinosinusitis AT alkisjamespsaltisphdmbbs characterizationofbacterialandfungalbiofilmsinchronicrhinosinusitis AT lorwaitanphd characterizationofbacterialandfungalbiofilmsinchronicrhinosinusitis AT peterjohnwormaldmd characterizationofbacterialandfungalbiofilmsinchronicrhinosinusitis |