Role of the domestic dog as a reservoir host of <it>Leishmania donovani </it>in eastern Sudan
<p>Abstract</p> <p>Background</p> <p>The study aims to determine the role of domestic dogs in transmission of visceral leishmaniasis in eastern Sudan. A cross-sectional survey was conducted in 10 villages along the River Rahad in eastern Sudan to elucidate the role of d...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-06-01
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| Series: | Parasites & Vectors |
| Online Access: | http://www.parasitesandvectors.com/content/2/1/26 |
| _version_ | 1811260501272821760 |
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| author | Schallig Henk DFH El-Raba'a Fathi MA Osman Omran F Hassan Mo'awia M Elnaiem Dia-Eldin A |
| author_facet | Schallig Henk DFH El-Raba'a Fathi MA Osman Omran F Hassan Mo'awia M Elnaiem Dia-Eldin A |
| author_sort | Schallig Henk DFH |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The study aims to determine the role of domestic dogs in transmission of visceral leishmaniasis in eastern Sudan. A cross-sectional survey was conducted in 10 villages along the River Rahad in eastern Sudan to elucidate the role of domestic dogs (<it>Canis familiaris</it>, Linnaeus, 1758) as a reservoir host of <it>Leishmania donovani</it>. In this study, 87 dogs were screened for infection by <it>Leishmania donovani</it>. Blood and lymph node samples were taken from 87 and 33 dogs respectively and subsequently screened by the Polymerase Chain Reaction (PCR) and Direct Agglutination Test (DAT) test. Additional lymph node smears were processed for microscopy and parasite culture. Host preference of the visceral leishmaniasis (VL) vector in the area, <it>Phlebotomus orientalis</it>, and other sandflies for the Nile rat (<it>Arvicanthis niloticus</it>, É. Geoffrey, 1803), the genet (<it>Genetta genetta</it>, Linnaeus, 1758), the mongoose (<it>Herpeistes ichneumon</it>, Linnaeus, 1758), and the domestic dog were determined by counting numbers of sand flies attracted to CDC traps that were baited by these animals.</p> <p>Results</p> <p>DAT on blood samples detected anti-<it>Leishmania </it>antibodies in 6 samples (6.9%). Two out of 87 (2.3%) blood samples tested were PCR positive, giving an amplification product of 560 bp. The two positive samples by PCR were also positive by DAT. However, none of the 33 lymph nodes aspirates were <it>Leishmania </it>positive when screened by microscopy, culture and genus-specific PCR. The dog-baited trap significantly attracted the highest number of <it>P. orientalis </it>and sand fly species (P < 0.001). This was followed by the Egyptian mongoose baited trap and less frequently by the genet baited trap.</p> <p>Conclusion</p> <p>It is concluded that the results obtained from host attraction studies indicate that dog is more attractive for <it>P. orientalis </it>than Egyptian mongoose, common genet and Nile rat.</p> |
| first_indexed | 2024-04-12T18:47:25Z |
| format | Article |
| id | doaj.art-97c960b7dd6e485886faa170575f333f |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-04-12T18:47:25Z |
| publishDate | 2009-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-97c960b7dd6e485886faa170575f333f2022-12-22T03:20:34ZengBMCParasites & Vectors1756-33052009-06-01212610.1186/1756-3305-2-26Role of the domestic dog as a reservoir host of <it>Leishmania donovani </it>in eastern SudanSchallig Henk DFHEl-Raba'a Fathi MAOsman Omran FHassan Mo'awia MElnaiem Dia-Eldin A<p>Abstract</p> <p>Background</p> <p>The study aims to determine the role of domestic dogs in transmission of visceral leishmaniasis in eastern Sudan. A cross-sectional survey was conducted in 10 villages along the River Rahad in eastern Sudan to elucidate the role of domestic dogs (<it>Canis familiaris</it>, Linnaeus, 1758) as a reservoir host of <it>Leishmania donovani</it>. In this study, 87 dogs were screened for infection by <it>Leishmania donovani</it>. Blood and lymph node samples were taken from 87 and 33 dogs respectively and subsequently screened by the Polymerase Chain Reaction (PCR) and Direct Agglutination Test (DAT) test. Additional lymph node smears were processed for microscopy and parasite culture. Host preference of the visceral leishmaniasis (VL) vector in the area, <it>Phlebotomus orientalis</it>, and other sandflies for the Nile rat (<it>Arvicanthis niloticus</it>, É. Geoffrey, 1803), the genet (<it>Genetta genetta</it>, Linnaeus, 1758), the mongoose (<it>Herpeistes ichneumon</it>, Linnaeus, 1758), and the domestic dog were determined by counting numbers of sand flies attracted to CDC traps that were baited by these animals.</p> <p>Results</p> <p>DAT on blood samples detected anti-<it>Leishmania </it>antibodies in 6 samples (6.9%). Two out of 87 (2.3%) blood samples tested were PCR positive, giving an amplification product of 560 bp. The two positive samples by PCR were also positive by DAT. However, none of the 33 lymph nodes aspirates were <it>Leishmania </it>positive when screened by microscopy, culture and genus-specific PCR. The dog-baited trap significantly attracted the highest number of <it>P. orientalis </it>and sand fly species (P < 0.001). This was followed by the Egyptian mongoose baited trap and less frequently by the genet baited trap.</p> <p>Conclusion</p> <p>It is concluded that the results obtained from host attraction studies indicate that dog is more attractive for <it>P. orientalis </it>than Egyptian mongoose, common genet and Nile rat.</p>http://www.parasitesandvectors.com/content/2/1/26 |
| spellingShingle | Schallig Henk DFH El-Raba'a Fathi MA Osman Omran F Hassan Mo'awia M Elnaiem Dia-Eldin A Role of the domestic dog as a reservoir host of <it>Leishmania donovani </it>in eastern Sudan Parasites & Vectors |
| title | Role of the domestic dog as a reservoir host of <it>Leishmania donovani </it>in eastern Sudan |
| title_full | Role of the domestic dog as a reservoir host of <it>Leishmania donovani </it>in eastern Sudan |
| title_fullStr | Role of the domestic dog as a reservoir host of <it>Leishmania donovani </it>in eastern Sudan |
| title_full_unstemmed | Role of the domestic dog as a reservoir host of <it>Leishmania donovani </it>in eastern Sudan |
| title_short | Role of the domestic dog as a reservoir host of <it>Leishmania donovani </it>in eastern Sudan |
| title_sort | role of the domestic dog as a reservoir host of it leishmania donovani it in eastern sudan |
| url | http://www.parasitesandvectors.com/content/2/1/26 |
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