A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley
All land plants seal their above ground body parts with a lipid-rich hydrophobic barrier called the cuticle to protect themselves from dehydration and other terrestrial threats. Mutational studies in several model species have identified multiple loci regulating cuticular metabolism and development....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/5/1088 |
| _version_ | 1797502259069714432 |
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| author | Trisha McAllister Chiara Campoli Mhmoud Eskan Linsan Liu Sarah M. McKim |
| author_facet | Trisha McAllister Chiara Campoli Mhmoud Eskan Linsan Liu Sarah M. McKim |
| author_sort | Trisha McAllister |
| collection | DOAJ |
| description | All land plants seal their above ground body parts with a lipid-rich hydrophobic barrier called the cuticle to protect themselves from dehydration and other terrestrial threats. Mutational studies in several model species have identified multiple loci regulating cuticular metabolism and development. Of particular importance are the <i>eceriferum</i> (<i>cer</i>) mutants characterized by a loss of cuticular wax. Some barley <i>cer</i> mutants, including <i>cer-x</i>, show defects in the distinctive β-diketone-enriched wax bloom on reproductive stage leaf sheaths, stems, and spikes. We exploited extensive allelic populations, near-isogenic lines, and powerful genotyping platforms to identify variation in the <i>HvWAX INDUCER1</i> (<i>HvWIN1</i>) gene, encoding a SHINE transcription factor, as underlying <i>cer-x</i>. Comparing the <i>cer-x</i> allelic <i>glossy sheath4.l</i> Bowman Near Isogenic Line BW407 to <i>cv</i>. Bowman revealed an increased cuticular permeability in tissues showing reduced accumulation of β-diketones and altered cuticular metabolic gene expression in BW407. Analyses across the barley pangenome and hundreds of exome-capture datasets revealed high sequence conservation of <i>HvWIN1</i> and two non-synonymous variants exclusive to the cultivated germplasm. Taken together, we suggest that variation in <i>HvWIN1</i> controls multiple cuticular features in barley. |
| first_indexed | 2024-03-10T03:30:24Z |
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| id | doaj.art-97ce796839fc495fb95af8a9b6ca8946 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T03:30:24Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-97ce796839fc495fb95af8a9b6ca89462023-11-23T09:42:37ZengMDPI AGAgronomy2073-43952022-04-01125108810.3390/agronomy12051088A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in BarleyTrisha McAllister0Chiara Campoli1Mhmoud Eskan2Linsan Liu3Sarah M. McKim4Division of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, UKDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, UKDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, UKDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, UKDivision of Plant Sciences, School of Life Sciences, University of Dundee at the James Hutton Institute, Dundee DD2 5DA, UKAll land plants seal their above ground body parts with a lipid-rich hydrophobic barrier called the cuticle to protect themselves from dehydration and other terrestrial threats. Mutational studies in several model species have identified multiple loci regulating cuticular metabolism and development. Of particular importance are the <i>eceriferum</i> (<i>cer</i>) mutants characterized by a loss of cuticular wax. Some barley <i>cer</i> mutants, including <i>cer-x</i>, show defects in the distinctive β-diketone-enriched wax bloom on reproductive stage leaf sheaths, stems, and spikes. We exploited extensive allelic populations, near-isogenic lines, and powerful genotyping platforms to identify variation in the <i>HvWAX INDUCER1</i> (<i>HvWIN1</i>) gene, encoding a SHINE transcription factor, as underlying <i>cer-x</i>. Comparing the <i>cer-x</i> allelic <i>glossy sheath4.l</i> Bowman Near Isogenic Line BW407 to <i>cv</i>. Bowman revealed an increased cuticular permeability in tissues showing reduced accumulation of β-diketones and altered cuticular metabolic gene expression in BW407. Analyses across the barley pangenome and hundreds of exome-capture datasets revealed high sequence conservation of <i>HvWIN1</i> and two non-synonymous variants exclusive to the cultivated germplasm. Taken together, we suggest that variation in <i>HvWIN1</i> controls multiple cuticular features in barley.https://www.mdpi.com/2073-4395/12/5/1088barleycuticle<i>eceriferum</i>waxSHINE transcription factor |
| spellingShingle | Trisha McAllister Chiara Campoli Mhmoud Eskan Linsan Liu Sarah M. McKim A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley Agronomy barley cuticle <i>eceriferum</i> wax SHINE transcription factor |
| title | A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley |
| title_full | A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley |
| title_fullStr | A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley |
| title_full_unstemmed | A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley |
| title_short | A Gene Encoding a SHINE1/WAX INDUCER1 Transcription Factor Controls Cuticular Wax in Barley |
| title_sort | gene encoding a shine1 wax inducer1 transcription factor controls cuticular wax in barley |
| topic | barley cuticle <i>eceriferum</i> wax SHINE transcription factor |
| url | https://www.mdpi.com/2073-4395/12/5/1088 |
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