Association of VLDLR haplotypes with abdominal fat trait in ducks
This study aimed to determine the correlation among <i>VLDLR</i> (very low-density lipoprotein receptor) gene polymorphisms, body weight and abdominal fat deposition of Gaoyou ducks. A total of 267 Gaoyou ducks from one pure line was employed for testing. The polymorphisms of the <...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2017-06-01
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| Series: | Archives Animal Breeding |
| Online Access: | http://www.arch-anim-breed.net/60/175/2017/aab-60-175-2017.pdf |
| _version_ | 1818197806799650816 |
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| author | S. Pan S. Pan S. Pan C. Wang C. Wang X. Dong M. Chen H. Xing H. Xing T. Zhang T. Zhang |
| author_facet | S. Pan S. Pan S. Pan C. Wang C. Wang X. Dong M. Chen H. Xing H. Xing T. Zhang T. Zhang |
| author_sort | S. Pan |
| collection | DOAJ |
| description | This study aimed to determine the correlation among
<i>VLDLR</i> (very
low-density lipoprotein receptor) gene polymorphisms, body weight and abdominal fat deposition of Gaoyou ducks. A total of 267 Gaoyou ducks from one pure line
was employed for testing. The polymorphisms of the <i>VLDLR</i> gene were screened by polymerase chain reaction and DNA sequencing. Four novel single nucleotide polymorphisms (SNPs) (g.151G > A, g.170C > T, g.206A > G and g.278–295del)
were identified in the 5'-UTR and signal peptide region. Furthermore, eight
haplotypes were identified based on the four SNPs. The H8 was the most common
haplotype with a frequency of more than 31 %. The four SNPs and their
haplotype combinations were shown to be significantly associated with body
weight at 6–10 weeks of age (<i>P</i> < 0. 05 or <i>P</i> < 0. 01) and
abdominal fat percentage (AFP) (<i>P</i> < 0. 05 or <i>P</i> < 0. 01).
Remarkably, the H1H1 diplotype had an effect on increasing body weight and
decreasing AFP from the 6th to the 10th weeks of age. However, increasing positive effects of the H5H8 diplotype were observed for both body weight and AFP. This study suggests that the <i>VLDLR</i> gene plays an important role in the regulation of body weight and fat-related traits and may serve as a potential marker for the marker-assisted selection program during duck breeding. |
| first_indexed | 2024-12-12T01:55:50Z |
| format | Article |
| id | doaj.art-97ce9a06cb3b4eeab0b3a3182f1f67d3 |
| institution | Directory Open Access Journal |
| issn | 0003-9438 2363-9822 |
| language | English |
| last_indexed | 2024-12-12T01:55:50Z |
| publishDate | 2017-06-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj.art-97ce9a06cb3b4eeab0b3a3182f1f67d32022-12-22T00:42:21ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222017-06-016017518210.5194/aab-60-175-2017Association of VLDLR haplotypes with abdominal fat trait in ducksS. Pan0S. Pan1S. Pan2C. Wang3C. Wang4X. Dong5M. Chen6H. Xing7H. Xing8T. Zhang9T. Zhang10College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, 225009, ChinaThese authors contributed equally to this work.College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, ChinaThese authors contributed equally to this work.College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, 225009, ChinaCollege of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, ChinaJiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, 225009, ChinaThis study aimed to determine the correlation among <i>VLDLR</i> (very low-density lipoprotein receptor) gene polymorphisms, body weight and abdominal fat deposition of Gaoyou ducks. A total of 267 Gaoyou ducks from one pure line was employed for testing. The polymorphisms of the <i>VLDLR</i> gene were screened by polymerase chain reaction and DNA sequencing. Four novel single nucleotide polymorphisms (SNPs) (g.151G > A, g.170C > T, g.206A > G and g.278–295del) were identified in the 5'-UTR and signal peptide region. Furthermore, eight haplotypes were identified based on the four SNPs. The H8 was the most common haplotype with a frequency of more than 31 %. The four SNPs and their haplotype combinations were shown to be significantly associated with body weight at 6–10 weeks of age (<i>P</i> < 0. 05 or <i>P</i> < 0. 01) and abdominal fat percentage (AFP) (<i>P</i> < 0. 05 or <i>P</i> < 0. 01). Remarkably, the H1H1 diplotype had an effect on increasing body weight and decreasing AFP from the 6th to the 10th weeks of age. However, increasing positive effects of the H5H8 diplotype were observed for both body weight and AFP. This study suggests that the <i>VLDLR</i> gene plays an important role in the regulation of body weight and fat-related traits and may serve as a potential marker for the marker-assisted selection program during duck breeding.http://www.arch-anim-breed.net/60/175/2017/aab-60-175-2017.pdf |
| spellingShingle | S. Pan S. Pan S. Pan C. Wang C. Wang X. Dong M. Chen H. Xing H. Xing T. Zhang T. Zhang Association of VLDLR haplotypes with abdominal fat trait in ducks Archives Animal Breeding |
| title | Association of VLDLR haplotypes with abdominal fat trait in ducks |
| title_full | Association of VLDLR haplotypes with abdominal fat trait in ducks |
| title_fullStr | Association of VLDLR haplotypes with abdominal fat trait in ducks |
| title_full_unstemmed | Association of VLDLR haplotypes with abdominal fat trait in ducks |
| title_short | Association of VLDLR haplotypes with abdominal fat trait in ducks |
| title_sort | association of vldlr haplotypes with abdominal fat trait in ducks |
| url | http://www.arch-anim-breed.net/60/175/2017/aab-60-175-2017.pdf |
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