POLYMORPHISM OF SOME TRANSCRIPTION FACTOR GENES RELATED TO DROUGHT TOLERANCE IN WHEAT
The aim of the research was to study polymorphism of preselected gene loci of three transcription factors (TaNAC2a, TaWRKY2, and TaWRKY19) and the Late Embryogenesis Abundant (LEA) proteins dehydrin (Td29b) related to wheat drought tolerance. The genes structure and chromosome location were establis...
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2018-08-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/storage/2_2018/lakhneko_2_2018.pdf |
| _version_ | 1797722640171925504 |
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| author | Lakhneko O. R. Stepanenko A. I. Kuzminskiy Ye. V. Morgun B. V. |
| author_facet | Lakhneko O. R. Stepanenko A. I. Kuzminskiy Ye. V. Morgun B. V. |
| author_sort | Lakhneko O. R. |
| collection | DOAJ |
| description | The aim of the research was to study polymorphism of preselected gene loci of three transcription factors (TaNAC2a, TaWRKY2, and TaWRKY19) and the Late Embryogenesis Abundant (LEA) proteins dehydrin (Td29b) related to wheat drought tolerance. The genes structure and chromosome location were established via bioinformatics tools. It is stated that TaWRKY2 and TaWRKY19 genes were comprised of 4 exons and 3 introns located on 2BS and 1DS chromosome arms, respectively; TaNAC2a — 2 exons and 1 intron 7AS; Td29b — single exon gene 3AS. Using polymerase chain reaction, no polymorphism was observed. Polymorphic bands were detected for TaWRKY2 locus. The screening of the distribution of the revealed polymorphic loci was carried out for a set of wheat and rye varieties, old landraces and interspecific hybrids. The polymorphism of TaWRKY2 locus indicated the presence of some other possible alleles of the gene. The obtained data are important for further investigations of wheat drought tolerance. |
| first_indexed | 2024-03-12T09:51:28Z |
| format | Article |
| id | doaj.art-97d241ff6ba6418c847de21e769dfcbb |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T09:51:28Z |
| publishDate | 2018-08-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-97d241ff6ba6418c847de21e769dfcbb2023-09-02T12:32:06ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2018-08-01112475610.15407/biotech11.02.047POLYMORPHISM OF SOME TRANSCRIPTION FACTOR GENES RELATED TO DROUGHT TOLERANCE IN WHEATLakhneko O. R.0Stepanenko A. I.1Kuzminskiy Ye. V.2Morgun B. V.3Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv; National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv, UkraineInstitute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv; School of Life Sciences, Huaiyin Normal University, Huaian, 223300, ChinaNational Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv, UkraineInstitute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv, National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv, UkraineThe aim of the research was to study polymorphism of preselected gene loci of three transcription factors (TaNAC2a, TaWRKY2, and TaWRKY19) and the Late Embryogenesis Abundant (LEA) proteins dehydrin (Td29b) related to wheat drought tolerance. The genes structure and chromosome location were established via bioinformatics tools. It is stated that TaWRKY2 and TaWRKY19 genes were comprised of 4 exons and 3 introns located on 2BS and 1DS chromosome arms, respectively; TaNAC2a — 2 exons and 1 intron 7AS; Td29b — single exon gene 3AS. Using polymerase chain reaction, no polymorphism was observed. Polymorphic bands were detected for TaWRKY2 locus. The screening of the distribution of the revealed polymorphic loci was carried out for a set of wheat and rye varieties, old landraces and interspecific hybrids. The polymorphism of TaWRKY2 locus indicated the presence of some other possible alleles of the gene. The obtained data are important for further investigations of wheat drought tolerance.http://biotechnology.kiev.ua/images/storage/2_2018/lakhneko_2_2018.pdfTriticum spp.polymerase chain reactiontranscription factorsTaNAC2aTaWRKY2TaWRKY19LEATd29bdrought tolerance |
| spellingShingle | Lakhneko O. R. Stepanenko A. I. Kuzminskiy Ye. V. Morgun B. V. POLYMORPHISM OF SOME TRANSCRIPTION FACTOR GENES RELATED TO DROUGHT TOLERANCE IN WHEAT Biotechnologia Acta Triticum spp. polymerase chain reaction transcription factors TaNAC2a TaWRKY2 TaWRKY19 LEA Td29b drought tolerance |
| title | POLYMORPHISM OF SOME TRANSCRIPTION FACTOR GENES RELATED TO DROUGHT TOLERANCE IN WHEAT |
| title_full | POLYMORPHISM OF SOME TRANSCRIPTION FACTOR GENES RELATED TO DROUGHT TOLERANCE IN WHEAT |
| title_fullStr | POLYMORPHISM OF SOME TRANSCRIPTION FACTOR GENES RELATED TO DROUGHT TOLERANCE IN WHEAT |
| title_full_unstemmed | POLYMORPHISM OF SOME TRANSCRIPTION FACTOR GENES RELATED TO DROUGHT TOLERANCE IN WHEAT |
| title_short | POLYMORPHISM OF SOME TRANSCRIPTION FACTOR GENES RELATED TO DROUGHT TOLERANCE IN WHEAT |
| title_sort | polymorphism of some transcription factor genes related to drought tolerance in wheat |
| topic | Triticum spp. polymerase chain reaction transcription factors TaNAC2a TaWRKY2 TaWRKY19 LEA Td29b drought tolerance |
| url | http://biotechnology.kiev.ua/images/storage/2_2018/lakhneko_2_2018.pdf |
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