Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs
The current study was designed to determine the prevalence of antimicrobial resistance and virulence genes in <em>Escherichia coli </em>isolated from canine diarrheal cases. Fecal samples were collected from 77 dogs with clinical diarrhea and 10 non-diarrheic dogs. Breed, age and sex, an...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | Arabic |
| Published: |
University of Mosul, College of Veterinary Medicine
2023-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://www.vetmedmosul.com/article_177307_518fc2ac67a1d512af2ddc24d53e5402.pdf |
| Summary: | The current study was designed to determine the prevalence of antimicrobial resistance and virulence genes in <em>Escherichia coli </em>isolated from canine diarrheal cases. Fecal samples were collected from 77 dogs with clinical diarrhea and 10 non-diarrheic dogs. Breed, age and sex, and clinical manifestations were recorded. Samples were plated on MacConkey and EMB agars, followed by standard isolation procedures and identification of <em>E. coli</em>. An antimicrobial susceptibility test using the disc diffusion method was performed against Ampicillin, Tetracycline, Trimethoprim, Gentamicin, and Streptomycin. PCR was used to determine if the isolates carry virulence intimin adherence protein (<em>eae</em>A) and antimicrobial resistance genes. Body temperature, respiratory, and heart rates in dogs with diarrhea were significantly higher than in non-diarrheic dogs. PCR detected the <em>eae</em>A virulence gene in 44(69.8%) of 63 isolates from diarrheic dogs. All isolates were resistant to ampicillin, and 55(87.3%), 47(74.6%), 29(46.0%), and 19(30.2%) of these were resistant to tetracycline, trimethoprim, gentamicin, and streptomycin, respectively. The frequency of antimicrobial resistance genes in the 63 isolates was 81.0, 52.4, 41.3, 33.3, 23.8 and 9.5% for <em>CITM</em>, <em>tet </em>(B), <em>dfrA1, aac (3)-IV</em>, <em>aadA1 </em>and<em> tet </em>(A), respectively. Overall, 6(9.4%), 16(25.4%), and 41(65.1%) were positive for one, two, three, or four resistance genes, respectively. In conclusion, the high prevalence of virulence (69.8%) and resistance 9.5-81.0% genes in <em>E. coli </em>isolates could be responsible for the diarrhea episodes, which may have posed therapeutic implications in affected dogs. |
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| ISSN: | 1607-3894 2071-1255 |