Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs
The current study was designed to determine the prevalence of antimicrobial resistance and virulence genes in <em>Escherichia coli </em>isolated from canine diarrheal cases. Fecal samples were collected from 77 dogs with clinical diarrhea and 10 non-diarrheic dogs. Breed, age and sex, an...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2023-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_177307_518fc2ac67a1d512af2ddc24d53e5402.pdf |
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| author | Hussein A. Naji Wessam M. Saleh Zainab A. Saud Thaer R. Mhahal Firas A. Alhasson Gopal Reddy Woubit Abebe |
| author_facet | Hussein A. Naji Wessam M. Saleh Zainab A. Saud Thaer R. Mhahal Firas A. Alhasson Gopal Reddy Woubit Abebe |
| author_sort | Hussein A. Naji |
| collection | DOAJ |
| description | The current study was designed to determine the prevalence of antimicrobial resistance and virulence genes in <em>Escherichia coli </em>isolated from canine diarrheal cases. Fecal samples were collected from 77 dogs with clinical diarrhea and 10 non-diarrheic dogs. Breed, age and sex, and clinical manifestations were recorded. Samples were plated on MacConkey and EMB agars, followed by standard isolation procedures and identification of <em>E. coli</em>. An antimicrobial susceptibility test using the disc diffusion method was performed against Ampicillin, Tetracycline, Trimethoprim, Gentamicin, and Streptomycin. PCR was used to determine if the isolates carry virulence intimin adherence protein (<em>eae</em>A) and antimicrobial resistance genes. Body temperature, respiratory, and heart rates in dogs with diarrhea were significantly higher than in non-diarrheic dogs. PCR detected the <em>eae</em>A virulence gene in 44(69.8%) of 63 isolates from diarrheic dogs. All isolates were resistant to ampicillin, and 55(87.3%), 47(74.6%), 29(46.0%), and 19(30.2%) of these were resistant to tetracycline, trimethoprim, gentamicin, and streptomycin, respectively. The frequency of antimicrobial resistance genes in the 63 isolates was 81.0, 52.4, 41.3, 33.3, 23.8 and 9.5% for <em>CITM</em>, <em>tet </em>(B), <em>dfrA1, aac (3)-IV</em>, <em>aadA1 </em>and<em> tet </em>(A), respectively. Overall, 6(9.4%), 16(25.4%), and 41(65.1%) were positive for one, two, three, or four resistance genes, respectively. In conclusion, the high prevalence of virulence (69.8%) and resistance 9.5-81.0% genes in <em>E. coli </em>isolates could be responsible for the diarrhea episodes, which may have posed therapeutic implications in affected dogs. |
| first_indexed | 2024-04-09T20:11:07Z |
| format | Article |
| id | doaj.art-97d58b53de474d10918dd5faee599a62 |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-04-09T20:11:07Z |
| publishDate | 2023-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-97d58b53de474d10918dd5faee599a622023-03-31T23:07:07ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552023-04-0137235536110.33899/ijvs.2022.133514.2243177307Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogsHussein A. Naji0Wessam M. Saleh1Zainab A. Saud2Thaer R. Mhahal3Firas A. Alhasson4Gopal Reddy5Woubit Abebe6Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Basrah.Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Basrah, Basrah, IraqDepartment of Public Health, College of Veterinary Medicine, University of Basrah, Basrah, IraqDepartment of Anatomy and Histology, College of Veterinary Medicine, University of Basrah, Basrah, IraqDepartment of Anatomy and Histology, College of Veterinary Medicine, University of Basrah, Basrah, IraqDepartment of Pathobiology, College of Veterinary Medicine, Tuskegee University, USADepartment of Pathobiology, College of Veterinary Medicine, Tuskegee University, USAThe current study was designed to determine the prevalence of antimicrobial resistance and virulence genes in <em>Escherichia coli </em>isolated from canine diarrheal cases. Fecal samples were collected from 77 dogs with clinical diarrhea and 10 non-diarrheic dogs. Breed, age and sex, and clinical manifestations were recorded. Samples were plated on MacConkey and EMB agars, followed by standard isolation procedures and identification of <em>E. coli</em>. An antimicrobial susceptibility test using the disc diffusion method was performed against Ampicillin, Tetracycline, Trimethoprim, Gentamicin, and Streptomycin. PCR was used to determine if the isolates carry virulence intimin adherence protein (<em>eae</em>A) and antimicrobial resistance genes. Body temperature, respiratory, and heart rates in dogs with diarrhea were significantly higher than in non-diarrheic dogs. PCR detected the <em>eae</em>A virulence gene in 44(69.8%) of 63 isolates from diarrheic dogs. All isolates were resistant to ampicillin, and 55(87.3%), 47(74.6%), 29(46.0%), and 19(30.2%) of these were resistant to tetracycline, trimethoprim, gentamicin, and streptomycin, respectively. The frequency of antimicrobial resistance genes in the 63 isolates was 81.0, 52.4, 41.3, 33.3, 23.8 and 9.5% for <em>CITM</em>, <em>tet </em>(B), <em>dfrA1, aac (3)-IV</em>, <em>aadA1 </em>and<em> tet </em>(A), respectively. Overall, 6(9.4%), 16(25.4%), and 41(65.1%) were positive for one, two, three, or four resistance genes, respectively. In conclusion, the high prevalence of virulence (69.8%) and resistance 9.5-81.0% genes in <em>E. coli </em>isolates could be responsible for the diarrhea episodes, which may have posed therapeutic implications in affected dogs.https://www.vetmedmosul.com/article_177307_518fc2ac67a1d512af2ddc24d53e5402.pdfe. coliresistance genesdiarrheic dogsantimicrobial resistance |
| spellingShingle | Hussein A. Naji Wessam M. Saleh Zainab A. Saud Thaer R. Mhahal Firas A. Alhasson Gopal Reddy Woubit Abebe Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs Iraqi Journal of Veterinary Sciences e. coli resistance genes diarrheic dogs antimicrobial resistance |
| title | Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs |
| title_full | Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs |
| title_fullStr | Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs |
| title_full_unstemmed | Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs |
| title_short | Prevalence of resistance and virulence genes in Escherichia coli isolates from diarrheic dogs |
| title_sort | prevalence of resistance and virulence genes in escherichia coli isolates from diarrheic dogs |
| topic | e. coli resistance genes diarrheic dogs antimicrobial resistance |
| url | https://www.vetmedmosul.com/article_177307_518fc2ac67a1d512af2ddc24d53e5402.pdf |
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