Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies
Sathish Sundar Dhilip Kumar, Nicolette Nadene Houreld, Heidi Abrahamse Laser Research Centre, University of Johannesburg, Johannesburg, South AfricaCorrespondence: Sathish Sundar Dhilip KumarLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, Jo...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2020-09-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/selective-laser-efficiency-of-green-synthesized-silver-nanoparticles-b-peer-reviewed-article-IJN |
| _version_ | 1818429234428772352 |
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| author | Dhilip Kumar SS Houreld NN Abrahamse H |
| author_facet | Dhilip Kumar SS Houreld NN Abrahamse H |
| author_sort | Dhilip Kumar SS |
| collection | DOAJ |
| description | Sathish Sundar Dhilip Kumar, Nicolette Nadene Houreld, Heidi Abrahamse Laser Research Centre, University of Johannesburg, Johannesburg, South AfricaCorrespondence: Sathish Sundar Dhilip KumarLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg 2028, South AfricaFax +27 11 559 6884Email sathishd@uj.ac.zaIntroduction: Silver nanoparticles (AgNPs) have been extensively used in wound healing applications owing to their valuable physicochemical and biological properties. The main objective of this study was to evaluate the combined effects of green-synthesized silver nanoparticles (G-AgNPs) and photobiomodulation (PBM; laser irradiation at 830 nm with 5 J/cm2) in normal wounded and diabetic wounded fibroblast cells (WS1).Methods: The combined effect of G-AgNPs and PBM was studied by various in vitro wound healing studies including cell morphology, cell migration rate and percentage wound closure, cell viability, cell proliferation, and filamentous (F)-actin and nuclear morphology staining.Results: Cell viability results revealed good cellular compatibility of G-AgNPs to WS1 cells. The combined therapy of G-AgNPs and PBM demonstrated promising results to achieve progressive migration and wound closure in both normal wounded and diabetic wounded cell models. G-AgNPs alone and in combination with PBM had no negative effect on cell viability and proliferation, and there was an increase in cell migration.Conclusion: Overall, these findings demonstrate that the combined treatment of G-AgNPs and PBM does not display any adverse effects on wound healing processes in both normal wounded and diabetic wounded cell models.Keywords: green synthesis, silver nanoparticles, photobiomodulation, laser, wound healing, cell migration |
| first_indexed | 2024-12-14T15:14:17Z |
| format | Article |
| id | doaj.art-97d5c5f573db4c48ab36bd98276c8afb |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T15:14:17Z |
| publishDate | 2020-09-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-97d5c5f573db4c48ab36bd98276c8afb2022-12-21T22:56:26ZengDove Medical PressInternational Journal of Nanomedicine1178-20132020-09-01Volume 156855687057109Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro StudiesDhilip Kumar SSHoureld NNAbrahamse HSathish Sundar Dhilip Kumar, Nicolette Nadene Houreld, Heidi Abrahamse Laser Research Centre, University of Johannesburg, Johannesburg, South AfricaCorrespondence: Sathish Sundar Dhilip KumarLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg 2028, South AfricaFax +27 11 559 6884Email sathishd@uj.ac.zaIntroduction: Silver nanoparticles (AgNPs) have been extensively used in wound healing applications owing to their valuable physicochemical and biological properties. The main objective of this study was to evaluate the combined effects of green-synthesized silver nanoparticles (G-AgNPs) and photobiomodulation (PBM; laser irradiation at 830 nm with 5 J/cm2) in normal wounded and diabetic wounded fibroblast cells (WS1).Methods: The combined effect of G-AgNPs and PBM was studied by various in vitro wound healing studies including cell morphology, cell migration rate and percentage wound closure, cell viability, cell proliferation, and filamentous (F)-actin and nuclear morphology staining.Results: Cell viability results revealed good cellular compatibility of G-AgNPs to WS1 cells. The combined therapy of G-AgNPs and PBM demonstrated promising results to achieve progressive migration and wound closure in both normal wounded and diabetic wounded cell models. G-AgNPs alone and in combination with PBM had no negative effect on cell viability and proliferation, and there was an increase in cell migration.Conclusion: Overall, these findings demonstrate that the combined treatment of G-AgNPs and PBM does not display any adverse effects on wound healing processes in both normal wounded and diabetic wounded cell models.Keywords: green synthesis, silver nanoparticles, photobiomodulation, laser, wound healing, cell migrationhttps://www.dovepress.com/selective-laser-efficiency-of-green-synthesized-silver-nanoparticles-b-peer-reviewed-article-IJNgreen synthesissilver nanoparticlesphotobiomodulationlaserwound healingcell migration |
| spellingShingle | Dhilip Kumar SS Houreld NN Abrahamse H Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies International Journal of Nanomedicine green synthesis silver nanoparticles photobiomodulation laser wound healing cell migration |
| title | Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies |
| title_full | Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies |
| title_fullStr | Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies |
| title_full_unstemmed | Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies |
| title_short | Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies |
| title_sort | selective laser efficiency of green synthesized silver nanoparticles by aloe arborescens and its wound healing activities in normal wounded and diabetic wounded fibroblast cells in vitro studies |
| topic | green synthesis silver nanoparticles photobiomodulation laser wound healing cell migration |
| url | https://www.dovepress.com/selective-laser-efficiency-of-green-synthesized-silver-nanoparticles-b-peer-reviewed-article-IJN |
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