Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation

In this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certai...

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Main Authors: Gunjo Kim, Janghwan Kim, Heejung Youn
Format: Article
Language:English
Published: MDPI AG 2018-08-01
Series:Applied Sciences
Subjects:
Online Access:http://www.mdpi.com/2076-3417/8/8/1277
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author Gunjo Kim
Janghwan Kim
Heejung Youn
author_facet Gunjo Kim
Janghwan Kim
Heejung Youn
author_sort Gunjo Kim
collection DOAJ
description In this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certain parameters such as (1) initial potential of hydrogen (pH) of urea-CaCl2 medium, (2) temperature during precipitation, and (3) the reaction duration. The pH values used for testing were 6, 7, 8, 9, and 10, the temperatures were 20, 30, 40, and 50 °C, and the reaction durations were 2, 3, and 4 days. Maximum calcite precipitation was observed at a pH of 7 and temperature of 30 °C. Most of the precipitation occurred within a reaction duration of 3 days. Under similar conditions, the amount of calcite precipitated by S. saprophyticus was estimated to be five times more than that by S. pasteurii. Both the species were sensitive to temperature; however, S. saprophyticus was less sensitive to pH and required a shorter reaction duration than S. pasteurii.
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spelling doaj.art-97dc1f9cca6747d58f2b6c3376fe806a2022-12-21T18:58:08ZengMDPI AGApplied Sciences2076-34172018-08-0188127710.3390/app8081277app8081277Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite PrecipitationGunjo Kim0Janghwan Kim1Heejung Youn2Department of Civil Engineering, Hongik University, Seoul 04066, KoreaCivil Design Team, Daelim Industrial Corp. Ltd., Seoul 08826, KoreaDepartment of Civil Engineering, Hongik University, Seoul 04066, KoreaIn this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certain parameters such as (1) initial potential of hydrogen (pH) of urea-CaCl2 medium, (2) temperature during precipitation, and (3) the reaction duration. The pH values used for testing were 6, 7, 8, 9, and 10, the temperatures were 20, 30, 40, and 50 °C, and the reaction durations were 2, 3, and 4 days. Maximum calcite precipitation was observed at a pH of 7 and temperature of 30 °C. Most of the precipitation occurred within a reaction duration of 3 days. Under similar conditions, the amount of calcite precipitated by S. saprophyticus was estimated to be five times more than that by S. pasteurii. Both the species were sensitive to temperature; however, S. saprophyticus was less sensitive to pH and required a shorter reaction duration than S. pasteurii.http://www.mdpi.com/2076-3417/8/8/1277MICPoptimal conditionStaphylococcus saprophyticusSporosarcina pasteurii
spellingShingle Gunjo Kim
Janghwan Kim
Heejung Youn
Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation
Applied Sciences
MICP
optimal condition
Staphylococcus saprophyticus
Sporosarcina pasteurii
title Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation
title_full Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation
title_fullStr Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation
title_full_unstemmed Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation
title_short Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation
title_sort effect of temperature ph and reaction duration on microbially induced calcite precipitation
topic MICP
optimal condition
Staphylococcus saprophyticus
Sporosarcina pasteurii
url http://www.mdpi.com/2076-3417/8/8/1277
work_keys_str_mv AT gunjokim effectoftemperaturephandreactiondurationonmicrobiallyinducedcalciteprecipitation
AT janghwankim effectoftemperaturephandreactiondurationonmicrobiallyinducedcalciteprecipitation
AT heejungyoun effectoftemperaturephandreactiondurationonmicrobiallyinducedcalciteprecipitation