Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation
In this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certai...
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MDPI AG
2018-08-01
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Online Access: | http://www.mdpi.com/2076-3417/8/8/1277 |
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author | Gunjo Kim Janghwan Kim Heejung Youn |
author_facet | Gunjo Kim Janghwan Kim Heejung Youn |
author_sort | Gunjo Kim |
collection | DOAJ |
description | In this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certain parameters such as (1) initial potential of hydrogen (pH) of urea-CaCl2 medium, (2) temperature during precipitation, and (3) the reaction duration. The pH values used for testing were 6, 7, 8, 9, and 10, the temperatures were 20, 30, 40, and 50 °C, and the reaction durations were 2, 3, and 4 days. Maximum calcite precipitation was observed at a pH of 7 and temperature of 30 °C. Most of the precipitation occurred within a reaction duration of 3 days. Under similar conditions, the amount of calcite precipitated by S. saprophyticus was estimated to be five times more than that by S. pasteurii. Both the species were sensitive to temperature; however, S. saprophyticus was less sensitive to pH and required a shorter reaction duration than S. pasteurii. |
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spelling | doaj.art-97dc1f9cca6747d58f2b6c3376fe806a2022-12-21T18:58:08ZengMDPI AGApplied Sciences2076-34172018-08-0188127710.3390/app8081277app8081277Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite PrecipitationGunjo Kim0Janghwan Kim1Heejung Youn2Department of Civil Engineering, Hongik University, Seoul 04066, KoreaCivil Design Team, Daelim Industrial Corp. Ltd., Seoul 08826, KoreaDepartment of Civil Engineering, Hongik University, Seoul 04066, KoreaIn this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certain parameters such as (1) initial potential of hydrogen (pH) of urea-CaCl2 medium, (2) temperature during precipitation, and (3) the reaction duration. The pH values used for testing were 6, 7, 8, 9, and 10, the temperatures were 20, 30, 40, and 50 °C, and the reaction durations were 2, 3, and 4 days. Maximum calcite precipitation was observed at a pH of 7 and temperature of 30 °C. Most of the precipitation occurred within a reaction duration of 3 days. Under similar conditions, the amount of calcite precipitated by S. saprophyticus was estimated to be five times more than that by S. pasteurii. Both the species were sensitive to temperature; however, S. saprophyticus was less sensitive to pH and required a shorter reaction duration than S. pasteurii.http://www.mdpi.com/2076-3417/8/8/1277MICPoptimal conditionStaphylococcus saprophyticusSporosarcina pasteurii |
spellingShingle | Gunjo Kim Janghwan Kim Heejung Youn Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation Applied Sciences MICP optimal condition Staphylococcus saprophyticus Sporosarcina pasteurii |
title | Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation |
title_full | Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation |
title_fullStr | Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation |
title_full_unstemmed | Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation |
title_short | Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation |
title_sort | effect of temperature ph and reaction duration on microbially induced calcite precipitation |
topic | MICP optimal condition Staphylococcus saprophyticus Sporosarcina pasteurii |
url | http://www.mdpi.com/2076-3417/8/8/1277 |
work_keys_str_mv | AT gunjokim effectoftemperaturephandreactiondurationonmicrobiallyinducedcalciteprecipitation AT janghwankim effectoftemperaturephandreactiondurationonmicrobiallyinducedcalciteprecipitation AT heejungyoun effectoftemperaturephandreactiondurationonmicrobiallyinducedcalciteprecipitation |