Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 Modulates Abscisic Acid Signaling by Inducing PYR1 Monomerization and Association With ABI1 in Arabidopsis
Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 (BAK1) is a versatile kinase involved in many different plant developmental responses. Previously, we showed that BAK1 interacts with open stomata 1 (OST1), a cytoplasmic kinase, to promote abscisic acid (ABA)-induced stomatal closure. ABA i...
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| Format: | Article |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.849467/full |
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| author | Yun Shang Yun Shang Dami Yang Yunmi Ha Yoon-Sun Hur Myeong Min Lee Kyoung Hee Nam Kyoung Hee Nam |
| author_facet | Yun Shang Yun Shang Dami Yang Yunmi Ha Yoon-Sun Hur Myeong Min Lee Kyoung Hee Nam Kyoung Hee Nam |
| author_sort | Yun Shang |
| collection | DOAJ |
| description | Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 (BAK1) is a versatile kinase involved in many different plant developmental responses. Previously, we showed that BAK1 interacts with open stomata 1 (OST1), a cytoplasmic kinase, to promote abscisic acid (ABA)-induced stomatal closure. ABA is a plant hormone that primarily regulates stress responses and is recognized by the PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENT OF ABA RECEPTORS (RCAR), which activates ABA signaling. Here, we demonstrated that BAK1 interacts with PYR1 and phosphorylates PYR1 in response to ABA in plants. We identified T137 and S142 of PYR1 as the phosphosites targeted by BAK1. Using phosphomimetic (PYR1DD) and phospho-dead (PYR1AA) PYR1 compared with wild-type PYR1, we showed that transgenic plants overexpressing a phosphomimetic PYR1 exhibited hypersensitivity to the inhibition of ABA-induced root growth and seed germination and increased ABA-induced stomatal closure and ABA-inducible gene expression. As underlying reasons for these phenomena, we further demonstrated that phosphorylated PYR1 existed in a monomeric form, in which ABA binding was increased, and the degree of complex formation with ABI1 was also increased. These results suggest that BAK1 positively modulates ABA signaling through interaction with PYR1, in addition to OST1. |
| first_indexed | 2024-12-10T09:42:22Z |
| format | Article |
| id | doaj.art-97df215ee941409bbff3ac6f2be45502 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T09:42:22Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-97df215ee941409bbff3ac6f2be455022022-12-22T01:53:58ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-04-011310.3389/fpls.2022.849467849467Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 Modulates Abscisic Acid Signaling by Inducing PYR1 Monomerization and Association With ABI1 in ArabidopsisYun Shang0Yun Shang1Dami Yang2Yunmi Ha3Yoon-Sun Hur4Myeong Min Lee5Kyoung Hee Nam6Kyoung Hee Nam7Department of Biological Sciences, Sookmyung Women’s University, Seoul, South KoreaResearch Institute of Women’s Health, Sookmyung Women’s University, Seoul, South KoreaDepartment of Biological Sciences, Sookmyung Women’s University, Seoul, South KoreaDepartment of Biological Sciences, Sookmyung Women’s University, Seoul, South KoreaDepartment of Systems Biology, Yonsei University, Seoul, South KoreaDepartment of Systems Biology, Yonsei University, Seoul, South KoreaDepartment of Biological Sciences, Sookmyung Women’s University, Seoul, South KoreaResearch Institute of Women’s Health, Sookmyung Women’s University, Seoul, South KoreaBrassinosteroid-Insensitive 1-Associated Receptor Kinase 1 (BAK1) is a versatile kinase involved in many different plant developmental responses. Previously, we showed that BAK1 interacts with open stomata 1 (OST1), a cytoplasmic kinase, to promote abscisic acid (ABA)-induced stomatal closure. ABA is a plant hormone that primarily regulates stress responses and is recognized by the PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENT OF ABA RECEPTORS (RCAR), which activates ABA signaling. Here, we demonstrated that BAK1 interacts with PYR1 and phosphorylates PYR1 in response to ABA in plants. We identified T137 and S142 of PYR1 as the phosphosites targeted by BAK1. Using phosphomimetic (PYR1DD) and phospho-dead (PYR1AA) PYR1 compared with wild-type PYR1, we showed that transgenic plants overexpressing a phosphomimetic PYR1 exhibited hypersensitivity to the inhibition of ABA-induced root growth and seed germination and increased ABA-induced stomatal closure and ABA-inducible gene expression. As underlying reasons for these phenomena, we further demonstrated that phosphorylated PYR1 existed in a monomeric form, in which ABA binding was increased, and the degree of complex formation with ABI1 was also increased. These results suggest that BAK1 positively modulates ABA signaling through interaction with PYR1, in addition to OST1.https://www.frontiersin.org/articles/10.3389/fpls.2022.849467/fullabscisic acidBrassinosteroid-Insensitive 1-Associated Receptor Kinase 1ABA-insensitive 1phosphorylationPYRABACTIN RESISTANCE1 |
| spellingShingle | Yun Shang Yun Shang Dami Yang Yunmi Ha Yoon-Sun Hur Myeong Min Lee Kyoung Hee Nam Kyoung Hee Nam Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 Modulates Abscisic Acid Signaling by Inducing PYR1 Monomerization and Association With ABI1 in Arabidopsis Frontiers in Plant Science abscisic acid Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 ABA-insensitive 1 phosphorylation PYRABACTIN RESISTANCE1 |
| title | Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 Modulates Abscisic Acid Signaling by Inducing PYR1 Monomerization and Association With ABI1 in Arabidopsis |
| title_full | Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 Modulates Abscisic Acid Signaling by Inducing PYR1 Monomerization and Association With ABI1 in Arabidopsis |
| title_fullStr | Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 Modulates Abscisic Acid Signaling by Inducing PYR1 Monomerization and Association With ABI1 in Arabidopsis |
| title_full_unstemmed | Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 Modulates Abscisic Acid Signaling by Inducing PYR1 Monomerization and Association With ABI1 in Arabidopsis |
| title_short | Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 Modulates Abscisic Acid Signaling by Inducing PYR1 Monomerization and Association With ABI1 in Arabidopsis |
| title_sort | brassinosteroid insensitive 1 associated receptor kinase 1 modulates abscisic acid signaling by inducing pyr1 monomerization and association with abi1 in arabidopsis |
| topic | abscisic acid Brassinosteroid-Insensitive 1-Associated Receptor Kinase 1 ABA-insensitive 1 phosphorylation PYRABACTIN RESISTANCE1 |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.849467/full |
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