Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’Ivoire
Several strains of a new aflatoxigenic species of Aspergillus, A. korhogoensis, were isolated in the course of a screening study involving species from section Flavi found contaminating peanuts (Arachis hypogaea) and peanut paste in the Côte d’Ivoire. Based on examination of four isolates, this new...
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| Format: | Article |
| Language: | English |
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MDPI AG
2017-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/9/11/353 |
| _version_ | 1798000060711043072 |
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| author | Amaranta Carvajal-Campos Ama Lethicia Manizan Souria Tadrist David Koffi Akaki Rose Koffi-Nevry Geromy G. Moore Stephen O. Fapohunda Sylviane Bailly Didier Montet Isabelle P. Oswald Sophie Lorber Catherine Brabet Olivier Puel |
| author_facet | Amaranta Carvajal-Campos Ama Lethicia Manizan Souria Tadrist David Koffi Akaki Rose Koffi-Nevry Geromy G. Moore Stephen O. Fapohunda Sylviane Bailly Didier Montet Isabelle P. Oswald Sophie Lorber Catherine Brabet Olivier Puel |
| author_sort | Amaranta Carvajal-Campos |
| collection | DOAJ |
| description | Several strains of a new aflatoxigenic species of Aspergillus, A. korhogoensis, were isolated in the course of a screening study involving species from section Flavi found contaminating peanuts (Arachis hypogaea) and peanut paste in the Côte d’Ivoire. Based on examination of four isolates, this new species is described using a polyphasic approach. A concatenated alignment comprised of nine genes (ITS, benA, cmdA, mcm7, amdS, rpb1, preB, ppgA, and preA) was subjected to phylogenetic analysis, and resulted in all four strains being inferred as a distinct clade. Characterization of mating type for each strain revealed A. korhogoensis as a heterothallic species, since three isolates exhibited a singular MAT1-1 locus and one isolate exhibited a singular MAT1-2 locus. Morphological and physiological characterizations were also performed based on their growth on various types of media. Their respective extrolite profiles were characterized using LC/HRMS, and showed that this new species is capable of producing B- and G-aflatoxins, aspergillic acid, cyclopiazonic acid, aflavarins, and asparasones, as well as other metabolites. Altogether, our results confirm the monophyly of A. korhogoensis, and strengthen its position in the A. flavus clade, as the sister taxon of A. parvisclerotigenus. |
| first_indexed | 2024-04-11T11:14:16Z |
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| id | doaj.art-97e0089bef8b4223a0fcba4ba3646a23 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T11:14:16Z |
| publishDate | 2017-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-97e0089bef8b4223a0fcba4ba3646a232022-12-22T04:27:19ZengMDPI AGToxins2072-66512017-10-0191135310.3390/toxins9110353toxins9110353Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’IvoireAmaranta Carvajal-Campos0Ama Lethicia Manizan1Souria Tadrist2David Koffi Akaki3Rose Koffi-Nevry4Geromy G. Moore5Stephen O. Fapohunda6Sylviane Bailly7Didier Montet8Isabelle P. Oswald9Sophie Lorber10Catherine Brabet11Olivier Puel12Toxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, 31027 Toulouse, FranceLaboratoire de Biotechnologie et Microbiologie des Aliments, UFR des Sciences et Technologie des Aliments, Université Nangui Abrogoua, 02 BP 801 Abidjan, Côte d’IvoireToxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, 31027 Toulouse, FranceLaboratoire des Procédés Industriels de Synthèse, de l’Environnement et des Energies Nouvelles, Département Génie Chimique et Agro-alimentaire, Institut National Polytechnique Félix Houphouët-Boigny, BP 1313 Yamoussoukro, Côte d’IvoireLaboratoire de Biotechnologie et Microbiologie des Aliments, UFR des Sciences et Technologie des Aliments, Université Nangui Abrogoua, 02 BP 801 Abidjan, Côte d’IvoireSouthern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, New Orleans, LA 70179, USADepartment of Microbiology, Babcock University, 121003 Ilishan Remo, NigeriaToxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, 31027 Toulouse, FranceCentre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)—Département PERSYST—UMR QualiSud, 34398 Montpellier, FranceToxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, 31027 Toulouse, FranceToxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, 31027 Toulouse, FranceCentre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD)—Département PERSYST—UMR QualiSud, 34398 Montpellier, FranceToxalim (Research Centre in Food Toxicology), Université de Toulouse, INRA, ENVT, INP-Purpan, UPS, 31027 Toulouse, FranceSeveral strains of a new aflatoxigenic species of Aspergillus, A. korhogoensis, were isolated in the course of a screening study involving species from section Flavi found contaminating peanuts (Arachis hypogaea) and peanut paste in the Côte d’Ivoire. Based on examination of four isolates, this new species is described using a polyphasic approach. A concatenated alignment comprised of nine genes (ITS, benA, cmdA, mcm7, amdS, rpb1, preB, ppgA, and preA) was subjected to phylogenetic analysis, and resulted in all four strains being inferred as a distinct clade. Characterization of mating type for each strain revealed A. korhogoensis as a heterothallic species, since three isolates exhibited a singular MAT1-1 locus and one isolate exhibited a singular MAT1-2 locus. Morphological and physiological characterizations were also performed based on their growth on various types of media. Their respective extrolite profiles were characterized using LC/HRMS, and showed that this new species is capable of producing B- and G-aflatoxins, aspergillic acid, cyclopiazonic acid, aflavarins, and asparasones, as well as other metabolites. Altogether, our results confirm the monophyly of A. korhogoensis, and strengthen its position in the A. flavus clade, as the sister taxon of A. parvisclerotigenus.https://www.mdpi.com/2072-6651/9/11/353Aspergillus section Flaviaflatoxinscyclopiazonic acidpolyphasic approachversicolorins |
| spellingShingle | Amaranta Carvajal-Campos Ama Lethicia Manizan Souria Tadrist David Koffi Akaki Rose Koffi-Nevry Geromy G. Moore Stephen O. Fapohunda Sylviane Bailly Didier Montet Isabelle P. Oswald Sophie Lorber Catherine Brabet Olivier Puel Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’Ivoire Toxins Aspergillus section Flavi aflatoxins cyclopiazonic acid polyphasic approach versicolorins |
| title | Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’Ivoire |
| title_full | Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’Ivoire |
| title_fullStr | Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’Ivoire |
| title_full_unstemmed | Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’Ivoire |
| title_short | Aspergillus korhogoensis, a Novel Aflatoxin Producing Species from the Côte d’Ivoire |
| title_sort | aspergillus korhogoensis a novel aflatoxin producing species from the cote d ivoire |
| topic | Aspergillus section Flavi aflatoxins cyclopiazonic acid polyphasic approach versicolorins |
| url | https://www.mdpi.com/2072-6651/9/11/353 |
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