In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich Fibrin
Platelet-rich fibrin (PRF), which has been shown to promote wound and bone regeneration, has demonstrated antimicrobial properties against periodontal pathogens. However, in veterinary medicine, no study has determined the antimicrobial effects of canine platelet-rich fibrin (cPRF). Therefore, this...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/24/3786 |
| _version_ | 1797382322145722368 |
|---|---|
| author | Ravisa Warin Preeyanat Vongchan Witaya Suriyasathaporn David C. Hall Ratchadaporn Boripun Wanna Suriyasathaporn |
| author_facet | Ravisa Warin Preeyanat Vongchan Witaya Suriyasathaporn David C. Hall Ratchadaporn Boripun Wanna Suriyasathaporn |
| author_sort | Ravisa Warin |
| collection | DOAJ |
| description | Platelet-rich fibrin (PRF), which has been shown to promote wound and bone regeneration, has demonstrated antimicrobial properties against periodontal pathogens. However, in veterinary medicine, no study has determined the antimicrobial effects of canine platelet-rich fibrin (cPRF). Therefore, this study aimed to determine the antimicrobial effect of cPRF against <i>E. coli</i> and <i>S. pseudintermedius</i> found in dogs’ wounds and against the standard strain <i>S. aureus</i>. Additionally, the mechanism of the existing antibacterial activity of cPRF, which involves the formation of reactive oxygen species (ROS), was tested. Blood samples from six dogs were processed for cPRF. The antimicrobial properties of three groups (growth control, cPRF, and drug control) were evaluated at 0.5, 4, 8, and 24 h using a time–kill assay. The killing mechanisms involving ROS were evaluated using horseradish peroxidase (HRP) to suppress ROS production in PRF (PRF-SR). Subsequently, tests for antimicrobial properties and ROS generation were compared to those of the growth control and cPRF groups. The results showed that cPRF had significant antimicrobial properties against <i>E. coli</i> but no antimicrobial properties against <i>S. pseudintermedius</i>. After the ROS suppression, PRF-SR did not show an antimicrobial property against <i>E. coli</i>. Moreover, cPRF-treated bacteria exhibited significantly greater intracellular ROS than PRF-SR. In conclusion, canine PRF showed an antimicrobial effect against <i>E. coli</i>, and its antibacterial mechanism was related to releasing ROS. |
| first_indexed | 2024-03-08T21:03:45Z |
| format | Article |
| id | doaj.art-97e330ef235f4dff996abf4921ddf7f8 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-08T21:03:45Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-97e330ef235f4dff996abf4921ddf7f82023-12-22T13:47:22ZengMDPI AGAnimals2076-26152023-12-011324378610.3390/ani13243786In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich FibrinRavisa Warin0Preeyanat Vongchan1Witaya Suriyasathaporn2David C. Hall3Ratchadaporn Boripun4Wanna Suriyasathaporn5Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandDepartment of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, ThailandFaculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandFaculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4Z1, CanadaAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat 80160, ThailandFaculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, ThailandPlatelet-rich fibrin (PRF), which has been shown to promote wound and bone regeneration, has demonstrated antimicrobial properties against periodontal pathogens. However, in veterinary medicine, no study has determined the antimicrobial effects of canine platelet-rich fibrin (cPRF). Therefore, this study aimed to determine the antimicrobial effect of cPRF against <i>E. coli</i> and <i>S. pseudintermedius</i> found in dogs’ wounds and against the standard strain <i>S. aureus</i>. Additionally, the mechanism of the existing antibacterial activity of cPRF, which involves the formation of reactive oxygen species (ROS), was tested. Blood samples from six dogs were processed for cPRF. The antimicrobial properties of three groups (growth control, cPRF, and drug control) were evaluated at 0.5, 4, 8, and 24 h using a time–kill assay. The killing mechanisms involving ROS were evaluated using horseradish peroxidase (HRP) to suppress ROS production in PRF (PRF-SR). Subsequently, tests for antimicrobial properties and ROS generation were compared to those of the growth control and cPRF groups. The results showed that cPRF had significant antimicrobial properties against <i>E. coli</i> but no antimicrobial properties against <i>S. pseudintermedius</i>. After the ROS suppression, PRF-SR did not show an antimicrobial property against <i>E. coli</i>. Moreover, cPRF-treated bacteria exhibited significantly greater intracellular ROS than PRF-SR. In conclusion, canine PRF showed an antimicrobial effect against <i>E. coli</i>, and its antibacterial mechanism was related to releasing ROS.https://www.mdpi.com/2076-2615/13/24/3786canine platelet-rich fibrinwound infectionregenerative medicinedogsantimicrobial effectsreactive oxygen species |
| spellingShingle | Ravisa Warin Preeyanat Vongchan Witaya Suriyasathaporn David C. Hall Ratchadaporn Boripun Wanna Suriyasathaporn In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich Fibrin Animals canine platelet-rich fibrin wound infection regenerative medicine dogs antimicrobial effects reactive oxygen species |
| title | In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich Fibrin |
| title_full | In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich Fibrin |
| title_fullStr | In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich Fibrin |
| title_full_unstemmed | In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich Fibrin |
| title_short | In Vitro Antimicrobial Properties and Their Mechanisms in Relation to Reactive Oxygen Species of Canine Platelet-Rich Fibrin |
| title_sort | in vitro antimicrobial properties and their mechanisms in relation to reactive oxygen species of canine platelet rich fibrin |
| topic | canine platelet-rich fibrin wound infection regenerative medicine dogs antimicrobial effects reactive oxygen species |
| url | https://www.mdpi.com/2076-2615/13/24/3786 |
| work_keys_str_mv | AT ravisawarin invitroantimicrobialpropertiesandtheirmechanismsinrelationtoreactiveoxygenspeciesofcanineplateletrichfibrin AT preeyanatvongchan invitroantimicrobialpropertiesandtheirmechanismsinrelationtoreactiveoxygenspeciesofcanineplateletrichfibrin AT witayasuriyasathaporn invitroantimicrobialpropertiesandtheirmechanismsinrelationtoreactiveoxygenspeciesofcanineplateletrichfibrin AT davidchall invitroantimicrobialpropertiesandtheirmechanismsinrelationtoreactiveoxygenspeciesofcanineplateletrichfibrin AT ratchadapornboripun invitroantimicrobialpropertiesandtheirmechanismsinrelationtoreactiveoxygenspeciesofcanineplateletrichfibrin AT wannasuriyasathaporn invitroantimicrobialpropertiesandtheirmechanismsinrelationtoreactiveoxygenspeciesofcanineplateletrichfibrin |