Posttranslational modification of Elongation Factor P from Staphylococcus aureus
Antibiotic‐resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF‐P) is a highly conserved elongation protein factor that plays an important role in prote...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-07-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.12901 |
| _version_ | 1797821930187784192 |
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| author | Alexander Golubev Luc Negroni Filipp Krasnovid Shamil Validov Gulnara Yusupova Marat Yusupov Konstantin Usachev |
| author_facet | Alexander Golubev Luc Negroni Filipp Krasnovid Shamil Validov Gulnara Yusupova Marat Yusupov Konstantin Usachev |
| author_sort | Alexander Golubev |
| collection | DOAJ |
| description | Antibiotic‐resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF‐P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF‐P undergoes unique posttranslational modifications in a stepwise manner to function correctly, but experimental information on EF‐P posttranslational modifications is currently lacking for S. aureus. Here, we expressed EF‐P in S. aureus to analyze its posttranslational modifications by mass spectrometry and report experimental proof of 5‐aminopentanol modification of S. aureus EF‐P. |
| first_indexed | 2024-03-13T09:59:57Z |
| format | Article |
| id | doaj.art-97e37172150348cf879427859a337414 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-13T09:59:57Z |
| publishDate | 2020-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-97e37172150348cf879427859a3374142023-05-23T06:51:07ZengWileyFEBS Open Bio2211-54632020-07-011071342134710.1002/2211-5463.12901Posttranslational modification of Elongation Factor P from Staphylococcus aureusAlexander Golubev0Luc Negroni1Filipp Krasnovid2Shamil Validov3Gulnara Yusupova4Marat Yusupov5Konstantin Usachev6Laboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian FederationInstitut de Génétique et de Biologie Moléculaire et Cellulaire CNRS UMR7104 INSERM U964 Université de Strasbourg Illkirch FranceLaboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian FederationLaboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian FederationDépartement de Biologie et de Génomique Structurales Institut de Génétique et de Biologie Moléculaire et Cellulaire CNRS UMR7104 INSERM U964 Université de Strasbourg Illkirch FranceLaboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian FederationLaboratory of Structural Biology Institute of Fundamental Medicine and Biology Kazan Federal University Kazan Russian FederationAntibiotic‐resistant Staphylococcus aureus is becoming a major burden on health care systems in many countries, necessitating the identification of new targets for antibiotic development. Elongation Factor P (EF‐P) is a highly conserved elongation protein factor that plays an important role in protein synthesis and bacteria virulence. EF‐P undergoes unique posttranslational modifications in a stepwise manner to function correctly, but experimental information on EF‐P posttranslational modifications is currently lacking for S. aureus. Here, we expressed EF‐P in S. aureus to analyze its posttranslational modifications by mass spectrometry and report experimental proof of 5‐aminopentanol modification of S. aureus EF‐P.https://doi.org/10.1002/2211-5463.12901EF‐Pposttranslational modificationsribosomestallingStaphylococcus aureus |
| spellingShingle | Alexander Golubev Luc Negroni Filipp Krasnovid Shamil Validov Gulnara Yusupova Marat Yusupov Konstantin Usachev Posttranslational modification of Elongation Factor P from Staphylococcus aureus FEBS Open Bio EF‐P posttranslational modifications ribosome stalling Staphylococcus aureus |
| title | Posttranslational modification of Elongation Factor P from Staphylococcus aureus |
| title_full | Posttranslational modification of Elongation Factor P from Staphylococcus aureus |
| title_fullStr | Posttranslational modification of Elongation Factor P from Staphylococcus aureus |
| title_full_unstemmed | Posttranslational modification of Elongation Factor P from Staphylococcus aureus |
| title_short | Posttranslational modification of Elongation Factor P from Staphylococcus aureus |
| title_sort | posttranslational modification of elongation factor p from staphylococcus aureus |
| topic | EF‐P posttranslational modifications ribosome stalling Staphylococcus aureus |
| url | https://doi.org/10.1002/2211-5463.12901 |
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