Pancreatic Cancer Cells Induce MicroRNA Deregulation in Platelets
Pancreatic cancer is a pathology with a high mortality rate since it is detected at advanced stages, so the search for early-stage diagnostic biomarkers is essential. Liquid biopsies are currently being explored for this purpose and educated platelets are a good candidate, since they are known to pr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11438 |
| _version_ | 1797479056528113664 |
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| author | Jorge Yassen Díaz-Blancas Ismael Dominguez-Rosado Carlos Chan-Nuñez Jorge Melendez-Zajgla Vilma Maldonado |
| author_facet | Jorge Yassen Díaz-Blancas Ismael Dominguez-Rosado Carlos Chan-Nuñez Jorge Melendez-Zajgla Vilma Maldonado |
| author_sort | Jorge Yassen Díaz-Blancas |
| collection | DOAJ |
| description | Pancreatic cancer is a pathology with a high mortality rate since it is detected at advanced stages, so the search for early-stage diagnostic biomarkers is essential. Liquid biopsies are currently being explored for this purpose and educated platelets are a good candidate, since they are known to present a bidirectional interaction with tumor cells. In this work, we analyzed the effects of platelets on cancer cells’ viability, as determined by MTT, migration using transwell assays, clonogenicity in soft agar and stemness by dilution assays and stem markers’ expression. We found that the co-culture of platelets and pancreatic cancer cells increased the proliferation and migration capacity of BXCP3 cells, augmented clonogenicity and induced higher levels of Nanog, Sox2 and Oct4 expression. As platelets can provide horizontal transfer of microRNAs, we also determined the differential expression of miRNAs in platelets obtained from a small cohort of pancreatic cancer patients and healthy subjects. We found clear differences in the expression of several miRNAs between platelets of patients with cancer healthy subjects. Moreover, when we analyzed microRNAs from the platelets of the pancreatic juice and blood derived from each of the cancer patients, interestingly we find differences between the blood- and pancreatic juice-derived platelets suggesting the presence of different subpopulations of platelets in cancer patients, which warrant further analysis. |
| first_indexed | 2024-03-09T21:40:21Z |
| format | Article |
| id | doaj.art-97e88f02bf98457a848b1bcd1292942a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:40:21Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-97e88f02bf98457a848b1bcd1292942a2023-11-23T20:33:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123191143810.3390/ijms231911438Pancreatic Cancer Cells Induce MicroRNA Deregulation in PlateletsJorge Yassen Díaz-Blancas0Ismael Dominguez-Rosado1Carlos Chan-Nuñez2Jorge Melendez-Zajgla3Vilma Maldonado4Posgrado en Ciencias Biológicas, UNAM, Mexico City 04510, MexicoDepartment of Surgery, National Institute of Health Sciences and Nutrition Salvador Zubirán, Mexico City 14080, MexicoDepartment of Surgery, National Institute of Health Sciences and Nutrition Salvador Zubirán, Mexico City 14080, MexicoFunctional Cancer Genomics Laboratory, Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City 14160, MexicoEpigenetics Laboratory (2), Instituto Nacional de Medicina Genómica (INMEGEN), Mexico City 14160, MexicoPancreatic cancer is a pathology with a high mortality rate since it is detected at advanced stages, so the search for early-stage diagnostic biomarkers is essential. Liquid biopsies are currently being explored for this purpose and educated platelets are a good candidate, since they are known to present a bidirectional interaction with tumor cells. In this work, we analyzed the effects of platelets on cancer cells’ viability, as determined by MTT, migration using transwell assays, clonogenicity in soft agar and stemness by dilution assays and stem markers’ expression. We found that the co-culture of platelets and pancreatic cancer cells increased the proliferation and migration capacity of BXCP3 cells, augmented clonogenicity and induced higher levels of Nanog, Sox2 and Oct4 expression. As platelets can provide horizontal transfer of microRNAs, we also determined the differential expression of miRNAs in platelets obtained from a small cohort of pancreatic cancer patients and healthy subjects. We found clear differences in the expression of several miRNAs between platelets of patients with cancer healthy subjects. Moreover, when we analyzed microRNAs from the platelets of the pancreatic juice and blood derived from each of the cancer patients, interestingly we find differences between the blood- and pancreatic juice-derived platelets suggesting the presence of different subpopulations of platelets in cancer patients, which warrant further analysis.https://www.mdpi.com/1422-0067/23/19/11438miRNAspancreas cancertumor-educated platelets |
| spellingShingle | Jorge Yassen Díaz-Blancas Ismael Dominguez-Rosado Carlos Chan-Nuñez Jorge Melendez-Zajgla Vilma Maldonado Pancreatic Cancer Cells Induce MicroRNA Deregulation in Platelets International Journal of Molecular Sciences miRNAs pancreas cancer tumor-educated platelets |
| title | Pancreatic Cancer Cells Induce MicroRNA Deregulation in Platelets |
| title_full | Pancreatic Cancer Cells Induce MicroRNA Deregulation in Platelets |
| title_fullStr | Pancreatic Cancer Cells Induce MicroRNA Deregulation in Platelets |
| title_full_unstemmed | Pancreatic Cancer Cells Induce MicroRNA Deregulation in Platelets |
| title_short | Pancreatic Cancer Cells Induce MicroRNA Deregulation in Platelets |
| title_sort | pancreatic cancer cells induce microrna deregulation in platelets |
| topic | miRNAs pancreas cancer tumor-educated platelets |
| url | https://www.mdpi.com/1422-0067/23/19/11438 |
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