Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook
<span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 0pt; text-indent: 0in;" class="Continutabstract"><span style="font-size: x-small;"><span lang="EN-US" style="font-family:...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2013-02-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://notulaebiologicae.ro/index.php/nsb/article/view/9009 |
| _version_ | 1828357870838611968 |
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| author | Archana SRIVASTAVA Aruna G. JOSHI |
| author_facet | Archana SRIVASTAVA Aruna G. JOSHI |
| author_sort | Archana SRIVASTAVA |
| collection | DOAJ |
| description | <span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 0pt; text-indent: 0in;" class="Continutabstract"><span style="font-size: x-small;"><span lang="EN-US" style="font-family: "Garamond Premr Pro","serif"; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Portulaca grandiflora</span><span lang="EN-US"><span style="font-family: Garamond Premr Pro Subh;">, an important medicinal plant was found to show the incidence of shoot tip necrosis (STN) in shoot cultures obtained from nodal explants in Murashige and Skoog (MS) medium with 3% sucrose along with 8 µM Kinetin and 4 µM BAP. This physiological disorder is primarily due to the deficiency of calcium and boron content in the medium. Thus in the present work varying concentration of boron which was used in the form of boric acid (0.1-0.4 mM), calcium gluconate (3-30 mM) and calcium chloride (3-30 mM) were incorporated in MS medium and its effect on STN was evaluated. The observations revealed that 18 mM calcium chloride was the optimum concentration for lowering STN in shoot cultures to 40% after four weeks.</span></span></span></p><span style="font-family: Times New Roman; font-size: small;"> </span> |
| first_indexed | 2024-04-14T03:22:00Z |
| format | Article |
| id | doaj.art-97efa0f4f9e34f04b98034bbd2bd5e74 |
| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-14T03:22:00Z |
| publishDate | 2013-02-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-97efa0f4f9e34f04b98034bbd2bd5e742022-12-22T02:15:17ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642013-02-015145497930Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora HookArchana SRIVASTAVA0Aruna G. JOSHI1he Maharaja Sayajirao University of Baroda, Faculty of Science, Department of Botany, Vadodara- 390002he Maharaja Sayajirao University of Baroda, Faculty of Science, Department of Botany, Vadodara- 390002<span style="font-family: Times New Roman; font-size: small;"> </span><p style="margin: 0in 0in 0pt; text-indent: 0in;" class="Continutabstract"><span style="font-size: x-small;"><span lang="EN-US" style="font-family: "Garamond Premr Pro","serif"; mso-bidi-font-family: "Garamond Premr Pro"; mso-bidi-font-style: italic;">Portulaca grandiflora</span><span lang="EN-US"><span style="font-family: Garamond Premr Pro Subh;">, an important medicinal plant was found to show the incidence of shoot tip necrosis (STN) in shoot cultures obtained from nodal explants in Murashige and Skoog (MS) medium with 3% sucrose along with 8 µM Kinetin and 4 µM BAP. This physiological disorder is primarily due to the deficiency of calcium and boron content in the medium. Thus in the present work varying concentration of boron which was used in the form of boric acid (0.1-0.4 mM), calcium gluconate (3-30 mM) and calcium chloride (3-30 mM) were incorporated in MS medium and its effect on STN was evaluated. The observations revealed that 18 mM calcium chloride was the optimum concentration for lowering STN in shoot cultures to 40% after four weeks.</span></span></span></p><span style="font-family: Times New Roman; font-size: small;"> </span>http://notulaebiologicae.ro/index.php/nsb/article/view/9009 |
| spellingShingle | Archana SRIVASTAVA Aruna G. JOSHI Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook Notulae Scientia Biologicae |
| title | Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook |
| title_full | Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook |
| title_fullStr | Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook |
| title_full_unstemmed | Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook |
| title_short | Control of Shoot Tip Necrosis in Shoot Cultures of Portulaca grandiflora Hook |
| title_sort | control of shoot tip necrosis in shoot cultures of portulaca grandiflora hook |
| url | http://notulaebiologicae.ro/index.php/nsb/article/view/9009 |
| work_keys_str_mv | AT archanasrivastava controlofshoottipnecrosisinshootculturesofportulacagrandiflorahook AT arunagjoshi controlofshoottipnecrosisinshootculturesofportulacagrandiflorahook |