Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties
Most of the methods of poly-lactic-glycolic acid (PLGA) preparation involve the passage through the emulsification of a PLGA organic solution in water followed by solvent evaporation or extraction. The choice of the droplet stabilizer during the emulsion step is critical for the dimensions and the s...
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| Format: | Article |
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MDPI AG
2018-11-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/16/11/447 |
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| author | Dalila Miele Silvia Rossi Giuseppina Sandri Barbara Vigani Milena Sorrenti Paolo Giunchedi Franca Ferrari Maria Cristina Bonferoni |
| author_facet | Dalila Miele Silvia Rossi Giuseppina Sandri Barbara Vigani Milena Sorrenti Paolo Giunchedi Franca Ferrari Maria Cristina Bonferoni |
| author_sort | Dalila Miele |
| collection | DOAJ |
| description | Most of the methods of poly-lactic-glycolic acid (PLGA) preparation involve the passage through the emulsification of a PLGA organic solution in water followed by solvent evaporation or extraction. The choice of the droplet stabilizer during the emulsion step is critical for the dimensions and the surface characteristics of the nanoparticles (NPs). In the present work, a recently described ionic amphiphilic chitosan derivative, chitosan oleate salt (CS-OA), was proposed for the first time to prepare PLGA NPs. A full factorial design was used to understand the effect of some formulation and preparation parameters on the NP dimensions and on encapsulation efficiency (EE%) of Nile red, used as a tracer. On the basis of the DoE study, curcumin loaded NPs were prepared, having 329 ± 42 nm dimensions and 68.75% EE%. The presence of a chitosan coating at the surface was confirmed by positive zeta potential and resulted in mucoadhesion behavior. The expected improvement of the interaction of the chitosan surface modified nanoparticles with cell membrane surface was confirmed in Caco-2 cell culture by the internalization of the loaded curcumin. |
| first_indexed | 2024-12-10T07:07:37Z |
| format | Article |
| id | doaj.art-97f0a7acb12948f1a7c26c0822005fc0 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-12-10T07:07:37Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-97f0a7acb12948f1a7c26c0822005fc02022-12-22T01:58:09ZengMDPI AGMarine Drugs1660-33972018-11-01161144710.3390/md16110447md16110447Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction PropertiesDalila Miele0Silvia Rossi1Giuseppina Sandri2Barbara Vigani3Milena Sorrenti4Paolo Giunchedi5Franca Ferrari6Maria Cristina Bonferoni7Department of Drug Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Chemistry and Pharmacy, University of Sassari, 07100 Sassari, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyDepartment of Drug Sciences, University of Pavia, 27100 Pavia, ItalyMost of the methods of poly-lactic-glycolic acid (PLGA) preparation involve the passage through the emulsification of a PLGA organic solution in water followed by solvent evaporation or extraction. The choice of the droplet stabilizer during the emulsion step is critical for the dimensions and the surface characteristics of the nanoparticles (NPs). In the present work, a recently described ionic amphiphilic chitosan derivative, chitosan oleate salt (CS-OA), was proposed for the first time to prepare PLGA NPs. A full factorial design was used to understand the effect of some formulation and preparation parameters on the NP dimensions and on encapsulation efficiency (EE%) of Nile red, used as a tracer. On the basis of the DoE study, curcumin loaded NPs were prepared, having 329 ± 42 nm dimensions and 68.75% EE%. The presence of a chitosan coating at the surface was confirmed by positive zeta potential and resulted in mucoadhesion behavior. The expected improvement of the interaction of the chitosan surface modified nanoparticles with cell membrane surface was confirmed in Caco-2 cell culture by the internalization of the loaded curcumin.https://www.mdpi.com/1660-3397/16/11/447chitosan oleate saltamphiphilic polymerPLGAnanoparticlesmucoadhesionCaco-2 cell culturenile redcurcumin |
| spellingShingle | Dalila Miele Silvia Rossi Giuseppina Sandri Barbara Vigani Milena Sorrenti Paolo Giunchedi Franca Ferrari Maria Cristina Bonferoni Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties Marine Drugs chitosan oleate salt amphiphilic polymer PLGA nanoparticles mucoadhesion Caco-2 cell culture nile red curcumin |
| title | Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties |
| title_full | Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties |
| title_fullStr | Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties |
| title_full_unstemmed | Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties |
| title_short | Chitosan Oleate Salt as an Amphiphilic Polymer for the Surface Modification of Poly-Lactic-Glycolic Acid (PLGA) Nanoparticles. Preliminary Studies of Mucoadhesion and Cell Interaction Properties |
| title_sort | chitosan oleate salt as an amphiphilic polymer for the surface modification of poly lactic glycolic acid plga nanoparticles preliminary studies of mucoadhesion and cell interaction properties |
| topic | chitosan oleate salt amphiphilic polymer PLGA nanoparticles mucoadhesion Caco-2 cell culture nile red curcumin |
| url | https://www.mdpi.com/1660-3397/16/11/447 |
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