Housing Temperature Modulates the Impact of Diet-Induced Rise in Fat Mass on Adipose Tissue Before and During Pregnancy in Rats
Aim: To investigate whether housing temperature influences rat adiposity, and the extent it is modified by diet and/or pregnancy. Housing temperature impacts on brown adipose tissue, that possess a unique uncoupling protein (UCP) 1, which, when activated by reduced ambient temperature, enables rapid...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.00209/full |
| _version_ | 1819209308561211392 |
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| author | Layla Albustanji Gabriela S. Perez Gabriela S. Perez Gabriela S. Perez Enas AlHarethi Peter Aldiss Ian Bloor Jairza M. Barreto-Medeiros Jairza M. Barreto-Medeiros Helen Budge Michael E. Symonds Michael E. Symonds Neele Dellschaft |
| author_facet | Layla Albustanji Gabriela S. Perez Gabriela S. Perez Gabriela S. Perez Enas AlHarethi Peter Aldiss Ian Bloor Jairza M. Barreto-Medeiros Jairza M. Barreto-Medeiros Helen Budge Michael E. Symonds Michael E. Symonds Neele Dellschaft |
| author_sort | Layla Albustanji |
| collection | DOAJ |
| description | Aim: To investigate whether housing temperature influences rat adiposity, and the extent it is modified by diet and/or pregnancy. Housing temperature impacts on brown adipose tissue, that possess a unique uncoupling protein (UCP) 1, which, when activated by reduced ambient temperature, enables rapid heat generation.Methods: We, therefore, examined whether the effects of dietary induced rise in fat mass on interscapular brown fat in female rats were dependent on housing temperature, and whether pregnancy further modulates the response. Four week old rats were either maintained at thermoneutrality (27°C) or at a “standard” cool temperature (20°C), and fed either a control or obesogenic (high in fat and sugar) diet until 10 weeks old. They were then either tissue sampled or mated with a male maintained under the same conditions. The remaining dams were tissue sampled at either 10 or 19 days gestation.Results: Diet had the greatest effect on fat mass at thermoneutrality although, by 19 days gestation, fat weight was similar between groups. Prior to mating, the abundance of UCP1 was higher at 20°C, but was similar between groups during pregnancy. UCP1 mRNA followed a similar pattern, with expression declining to a greater extent in the animals maintained at 20°C.Conclusion: Housing temperature has a marked influence on the effect of dietary induced rise in fat deposition that was modified through gestation. This maybe mediated by the reduction in UCP1 with housing at thermoneutrality prior to pregnancy and could subsequently impact on growth and development of the offspring. |
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| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-23T05:53:13Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-97f30ab7432f4c959bdb609b7d85d3b82022-12-21T17:57:54ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-03-011010.3389/fphys.2019.00209431168Housing Temperature Modulates the Impact of Diet-Induced Rise in Fat Mass on Adipose Tissue Before and During Pregnancy in RatsLayla Albustanji0Gabriela S. Perez1Gabriela S. Perez2Gabriela S. Perez3Enas AlHarethi4Peter Aldiss5Ian Bloor6Jairza M. Barreto-Medeiros7Jairza M. Barreto-Medeiros8Helen Budge9Michael E. Symonds10Michael E. Symonds11Neele Dellschaft12Early Life Research Unit, Division of Child Health, Obstetrics, and Gynaecology, University of Nottingham, Nottingham, United KingdomEarly Life Research Unit, Division of Child Health, Obstetrics, and Gynaecology, University of Nottingham, Nottingham, United KingdomGraduate Program of Food Nutrition and Health, Department of Food Science, School of Nutrition, Federal University of Bahia, Salvador, BrazilCAPES Foundation, Ministry of Education of Brazil, Brasília, BrazilEarly Life Research Unit, Division of Child Health, Obstetrics, and Gynaecology, University of Nottingham, Nottingham, United KingdomEarly Life Research Unit, Division of Child Health, Obstetrics, and Gynaecology, University of Nottingham, Nottingham, United KingdomEarly Life Research Unit, Division of Child Health, Obstetrics, and Gynaecology, University of Nottingham, Nottingham, United KingdomGraduate Program of Food Nutrition and Health, Department of Food Science, School of Nutrition, Federal University of Bahia, Salvador, BrazilCAPES Foundation, Ministry of Education of Brazil, Brasília, BrazilEarly Life Research Unit, Division of Child Health, Obstetrics, and Gynaecology, University of Nottingham, Nottingham, United KingdomEarly Life Research Unit, Division of Child Health, Obstetrics, and Gynaecology, University of Nottingham, Nottingham, United KingdomNottingham Digestive Disease Centre and Biomedical Research Centre, School of Medicine, University of Nottingham, Nottingham, United KingdomEarly Life Research Unit, Division of Child Health, Obstetrics, and Gynaecology, University of Nottingham, Nottingham, United KingdomAim: To investigate whether housing temperature influences rat adiposity, and the extent it is modified by diet and/or pregnancy. Housing temperature impacts on brown adipose tissue, that possess a unique uncoupling protein (UCP) 1, which, when activated by reduced ambient temperature, enables rapid heat generation.Methods: We, therefore, examined whether the effects of dietary induced rise in fat mass on interscapular brown fat in female rats were dependent on housing temperature, and whether pregnancy further modulates the response. Four week old rats were either maintained at thermoneutrality (27°C) or at a “standard” cool temperature (20°C), and fed either a control or obesogenic (high in fat and sugar) diet until 10 weeks old. They were then either tissue sampled or mated with a male maintained under the same conditions. The remaining dams were tissue sampled at either 10 or 19 days gestation.Results: Diet had the greatest effect on fat mass at thermoneutrality although, by 19 days gestation, fat weight was similar between groups. Prior to mating, the abundance of UCP1 was higher at 20°C, but was similar between groups during pregnancy. UCP1 mRNA followed a similar pattern, with expression declining to a greater extent in the animals maintained at 20°C.Conclusion: Housing temperature has a marked influence on the effect of dietary induced rise in fat deposition that was modified through gestation. This maybe mediated by the reduction in UCP1 with housing at thermoneutrality prior to pregnancy and could subsequently impact on growth and development of the offspring.https://www.frontiersin.org/article/10.3389/fphys.2019.00209/fullthermogenesisbrown fatobesitytemperaturepregnancy |
| spellingShingle | Layla Albustanji Gabriela S. Perez Gabriela S. Perez Gabriela S. Perez Enas AlHarethi Peter Aldiss Ian Bloor Jairza M. Barreto-Medeiros Jairza M. Barreto-Medeiros Helen Budge Michael E. Symonds Michael E. Symonds Neele Dellschaft Housing Temperature Modulates the Impact of Diet-Induced Rise in Fat Mass on Adipose Tissue Before and During Pregnancy in Rats Frontiers in Physiology thermogenesis brown fat obesity temperature pregnancy |
| title | Housing Temperature Modulates the Impact of Diet-Induced Rise in Fat Mass on Adipose Tissue Before and During Pregnancy in Rats |
| title_full | Housing Temperature Modulates the Impact of Diet-Induced Rise in Fat Mass on Adipose Tissue Before and During Pregnancy in Rats |
| title_fullStr | Housing Temperature Modulates the Impact of Diet-Induced Rise in Fat Mass on Adipose Tissue Before and During Pregnancy in Rats |
| title_full_unstemmed | Housing Temperature Modulates the Impact of Diet-Induced Rise in Fat Mass on Adipose Tissue Before and During Pregnancy in Rats |
| title_short | Housing Temperature Modulates the Impact of Diet-Induced Rise in Fat Mass on Adipose Tissue Before and During Pregnancy in Rats |
| title_sort | housing temperature modulates the impact of diet induced rise in fat mass on adipose tissue before and during pregnancy in rats |
| topic | thermogenesis brown fat obesity temperature pregnancy |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.00209/full |
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