Ectopic Expression of OsJAZs Alters Plant Defense and Development
A key step in jasmonic acid (JA) signaling is the ligand-dependent assembly of a coreceptor complex comprising the F-box protein COI1 and JAZ transcriptional repressors. The assembly of this receptor complex results in proteasome-mediated degradation of JAZ repressors, which in turn bind and repress...
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/9/4581 |
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| author | Baolong Sun Luyue Shang Yang Li Qiang Zhang Zhaohui Chu Shengyang He Wei Yang Xinhua Ding |
| author_facet | Baolong Sun Luyue Shang Yang Li Qiang Zhang Zhaohui Chu Shengyang He Wei Yang Xinhua Ding |
| author_sort | Baolong Sun |
| collection | DOAJ |
| description | A key step in jasmonic acid (JA) signaling is the ligand-dependent assembly of a coreceptor complex comprising the F-box protein COI1 and JAZ transcriptional repressors. The assembly of this receptor complex results in proteasome-mediated degradation of JAZ repressors, which in turn bind and repress MYC transcription factors. Many studies on JAZs have been performed in <i>Arabidopsis thaliana</i>, but the function of JAZs in rice is largely unknown. To systematically reveal the function of OsJAZs, in this study, we compared the various phenotypes resulting from 13 <i>OsJAZs</i> via ectopic expression in <i>Arabidopsis thaliana</i> and the phenotypes of 12 <i>AtJAZs</i> overexpression (OE) lines. Phylogenetic analysis showed that the 25 proteins could be divided into three major groups. Yeast two-hybrid (Y2H) assays revealed that most OsJAZ proteins could form homodimers or heterodimers. The statistical results showed that the phenotypes of the <i>OsJAZ</i> OE plants were quite different from those of <i>AtJAZ</i> OE plants in terms of plant growth, development, and immunity. As an example, compared with other <i>JAZ</i> OE plants, <i>OsJAZ11</i> OE plants exhibited a JA-insensitive phenotype and enhanced resistance to <i>Pst</i> DC3000. The protein stability after JA treatment of OsJAZ11 emphasized the specific function of the protein. This study aimed to explore the commonalities and characteristics of different JAZ proteins functions from a genetic perspective, and to screen genes with disease resistance value. Overall, the results of this study provide insights for further functional analysis of rice JAZ family proteins. |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-97f6f58e797e4917bafaf5b8622e31462023-11-23T08:19:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01239458110.3390/ijms23094581Ectopic Expression of OsJAZs Alters Plant Defense and DevelopmentBaolong Sun0Luyue Shang1Yang Li2Qiang Zhang3Zhaohui Chu4Shengyang He5Wei Yang6Xinhua Ding7State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaDepartment of Biology, Duke University, Durham, NC 27708, USAState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, College of Plant Protection, Shandong Agricultural University, Tai’an 271018, ChinaA key step in jasmonic acid (JA) signaling is the ligand-dependent assembly of a coreceptor complex comprising the F-box protein COI1 and JAZ transcriptional repressors. The assembly of this receptor complex results in proteasome-mediated degradation of JAZ repressors, which in turn bind and repress MYC transcription factors. Many studies on JAZs have been performed in <i>Arabidopsis thaliana</i>, but the function of JAZs in rice is largely unknown. To systematically reveal the function of OsJAZs, in this study, we compared the various phenotypes resulting from 13 <i>OsJAZs</i> via ectopic expression in <i>Arabidopsis thaliana</i> and the phenotypes of 12 <i>AtJAZs</i> overexpression (OE) lines. Phylogenetic analysis showed that the 25 proteins could be divided into three major groups. Yeast two-hybrid (Y2H) assays revealed that most OsJAZ proteins could form homodimers or heterodimers. The statistical results showed that the phenotypes of the <i>OsJAZ</i> OE plants were quite different from those of <i>AtJAZ</i> OE plants in terms of plant growth, development, and immunity. As an example, compared with other <i>JAZ</i> OE plants, <i>OsJAZ11</i> OE plants exhibited a JA-insensitive phenotype and enhanced resistance to <i>Pst</i> DC3000. The protein stability after JA treatment of OsJAZ11 emphasized the specific function of the protein. This study aimed to explore the commonalities and characteristics of different JAZ proteins functions from a genetic perspective, and to screen genes with disease resistance value. Overall, the results of this study provide insights for further functional analysis of rice JAZ family proteins.https://www.mdpi.com/1422-0067/23/9/4581jasmonic acidtranscriptional repressorsJA insensitivefloweringroot growthplant immunity |
| spellingShingle | Baolong Sun Luyue Shang Yang Li Qiang Zhang Zhaohui Chu Shengyang He Wei Yang Xinhua Ding Ectopic Expression of OsJAZs Alters Plant Defense and Development International Journal of Molecular Sciences jasmonic acid transcriptional repressors JA insensitive flowering root growth plant immunity |
| title | Ectopic Expression of OsJAZs Alters Plant Defense and Development |
| title_full | Ectopic Expression of OsJAZs Alters Plant Defense and Development |
| title_fullStr | Ectopic Expression of OsJAZs Alters Plant Defense and Development |
| title_full_unstemmed | Ectopic Expression of OsJAZs Alters Plant Defense and Development |
| title_short | Ectopic Expression of OsJAZs Alters Plant Defense and Development |
| title_sort | ectopic expression of osjazs alters plant defense and development |
| topic | jasmonic acid transcriptional repressors JA insensitive flowering root growth plant immunity |
| url | https://www.mdpi.com/1422-0067/23/9/4581 |
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