<i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>)
This study presents a novel <i>Janthinobacterium</i> strain, SNU WT3, isolated from the kidney of rainbow trout. A phylogenetic study using 16S rRNA sequences indicated that the strain is closely related to <i>Janthinobacterium svalbardensis</i> JA-1<sup>T</sup>....
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/2/229 |
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| author | Won Joon Jung Sang Wha Kim Sib Sankar Giri Hyoun Joong Kim Sang Guen Kim Jeong Woo Kang Jun Kwon Sung Bin Lee Woo Taek Oh Jin Woo Jun Se Chang Park |
| author_facet | Won Joon Jung Sang Wha Kim Sib Sankar Giri Hyoun Joong Kim Sang Guen Kim Jeong Woo Kang Jun Kwon Sung Bin Lee Woo Taek Oh Jin Woo Jun Se Chang Park |
| author_sort | Won Joon Jung |
| collection | DOAJ |
| description | This study presents a novel <i>Janthinobacterium</i> strain, SNU WT3, isolated from the kidney of rainbow trout. A phylogenetic study using 16S rRNA sequences indicated that the strain is closely related to <i>Janthinobacterium svalbardensis</i> JA-1<sup>T</sup>. However, biochemical analysis found differences in D-xylose adonitol, N-acetylglucosamine, arbutin, and cellobiose. As for genome-to-genome distance and average nucleotide identity values calculated between strain SNU WT3 and other related strains such as <i>J. lividum</i> EIF1, <i>J. svalbardensis</i> PAMC 27463, and <i>J. agaricidamnosum</i> BHSEK were all below the cutoff value between species. DNA-DNA hybridization between strain SNU WT3 and other close relatives indicated the results of <i>J. lividum</i> DSM 1522<sup>T</sup> (47.11%) and <i>J. svalbardensis</i> JA-1<sup>T</sup> (38.88%) individually. The major fatty acid compositions of strain SNU WT3 were cylco-C<sub>17:0</sub> (41.45%), C<sub>16:0</sub> (33.86%) and C<sub>12:0</sub> (5.87%). The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, and diphosphatidylglycerol. The quinone system was composed mainly of ubiquinone Q-8. The genome of strain SNU WT3 consists of 6,314,370 bp with a G + C content of 62.35%. Here, we describe a novel species of the genus <i>Janthinobacterium</i>, and the name <i>Janthinobacterium tructae</i> has been proposed with SNU WT3<sup>T</sup> (=KCTC 72518 = JCM 33613) as the type strain. |
| first_indexed | 2024-03-09T00:44:51Z |
| format | Article |
| id | doaj.art-97f7a75e888c44c9acfe8513486f199c |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T00:44:51Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-97f7a75e888c44c9acfe8513486f199c2023-12-11T17:36:51ZengMDPI AGPathogens2076-08172021-02-0110222910.3390/pathogens10020229<i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>)Won Joon Jung0Sang Wha Kim1Sib Sankar Giri2Hyoun Joong Kim3Sang Guen Kim4Jeong Woo Kang5Jun Kwon6Sung Bin Lee7Woo Taek Oh8Jin Woo Jun9Se Chang Park10Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaDepartment of Aquaculture, Korea National College of Agriculture and Fisheries, Jeonju 54874, KoreaLaboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, KoreaThis study presents a novel <i>Janthinobacterium</i> strain, SNU WT3, isolated from the kidney of rainbow trout. A phylogenetic study using 16S rRNA sequences indicated that the strain is closely related to <i>Janthinobacterium svalbardensis</i> JA-1<sup>T</sup>. However, biochemical analysis found differences in D-xylose adonitol, N-acetylglucosamine, arbutin, and cellobiose. As for genome-to-genome distance and average nucleotide identity values calculated between strain SNU WT3 and other related strains such as <i>J. lividum</i> EIF1, <i>J. svalbardensis</i> PAMC 27463, and <i>J. agaricidamnosum</i> BHSEK were all below the cutoff value between species. DNA-DNA hybridization between strain SNU WT3 and other close relatives indicated the results of <i>J. lividum</i> DSM 1522<sup>T</sup> (47.11%) and <i>J. svalbardensis</i> JA-1<sup>T</sup> (38.88%) individually. The major fatty acid compositions of strain SNU WT3 were cylco-C<sub>17:0</sub> (41.45%), C<sub>16:0</sub> (33.86%) and C<sub>12:0</sub> (5.87%). The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, and diphosphatidylglycerol. The quinone system was composed mainly of ubiquinone Q-8. The genome of strain SNU WT3 consists of 6,314,370 bp with a G + C content of 62.35%. Here, we describe a novel species of the genus <i>Janthinobacterium</i>, and the name <i>Janthinobacterium tructae</i> has been proposed with SNU WT3<sup>T</sup> (=KCTC 72518 = JCM 33613) as the type strain.https://www.mdpi.com/2076-0817/10/2/229<i>Jantinobacterium</i><i>Jantinobacterium tructae</i>rainbow trout<i>Oncorhynchus mykiss</i> |
| spellingShingle | Won Joon Jung Sang Wha Kim Sib Sankar Giri Hyoun Joong Kim Sang Guen Kim Jeong Woo Kang Jun Kwon Sung Bin Lee Woo Taek Oh Jin Woo Jun Se Chang Park <i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) Pathogens <i>Jantinobacterium</i> <i>Jantinobacterium tructae</i> rainbow trout <i>Oncorhynchus mykiss</i> |
| title | <i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_full | <i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_fullStr | <i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_full_unstemmed | <i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_short | <i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_sort | i janthinobacterium tructae i sp nov isolated from kidney of rainbow trout i oncorhynchus mykiss i |
| topic | <i>Jantinobacterium</i> <i>Jantinobacterium tructae</i> rainbow trout <i>Oncorhynchus mykiss</i> |
| url | https://www.mdpi.com/2076-0817/10/2/229 |
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