Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine
Quorum sensing (QS) is a bacterial cell-cell communication system with genetically regulated mechanisms dependent on cell density. Canonical QS systems in gram-negative bacteria possess an autoinducer synthase (LuxI family) and a transcriptional regulator (LuxR family) that respond to an autoinducer...
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1040932/full |
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| author | Martha Patricia Chávez-Moctezuma Ramiro Martínez-Cámara Ramiro Martínez-Cámara Julie Hernández-Salmerón Gabriel Moreno-Hagelsieb Gustavo Santoyo Eduardo Valencia-Cantero |
| author_facet | Martha Patricia Chávez-Moctezuma Ramiro Martínez-Cámara Ramiro Martínez-Cámara Julie Hernández-Salmerón Gabriel Moreno-Hagelsieb Gustavo Santoyo Eduardo Valencia-Cantero |
| author_sort | Martha Patricia Chávez-Moctezuma |
| collection | DOAJ |
| description | Quorum sensing (QS) is a bacterial cell-cell communication system with genetically regulated mechanisms dependent on cell density. Canonical QS systems in gram-negative bacteria possess an autoinducer synthase (LuxI family) and a transcriptional regulator (LuxR family) that respond to an autoinducer molecule. In Gram-positive bacteria, the LuxR transcriptional regulators “solo” (not associated with a LuxI homolog) may play key roles in intracellular communication. Arthrobacter sp. UMCV2 is an actinobacterium that promotes plant growth by emitting the volatile organic compound N, N-dimethylhexadecylamine (DMHDA). This compound induces iron deficiency, defense responses in plants, and swarming motility in Arthrobacter sp. UMCV2. In this study, the draft genome of this bacterium was assembled and compared with the genomes of type strains of the Arthrobacter genus, finding that it does not belong to any previously described species. Genome explorations also revealed the presence of 16 luxR-related genes, but no luxI homologs were discovered. Eleven of these sequences possess the LuxR characteristic DNA-binding domain with a helix-turn-helix motif and were designated as auto-inducer-related regulators (AirR). Four sequences possessed LuxR analogous domains and were designated as auto-inducer analogous regulators (AiaR). When swarming motility was induced with DMHDA, eight airR genes and two aiaR genes were upregulated. These results indicate that the expression of multiple luxR-related genes is induced in actinobacteria, such as Arthrobacter sp. UMCV2, by the action of the bacterial biocompound DMHDA when QS behavior is produced. |
| first_indexed | 2024-04-12T17:49:18Z |
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| id | doaj.art-97fa2fa697d9441eaac9136f3f7a6d6d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T17:49:18Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-97fa2fa697d9441eaac9136f3f7a6d6d2022-12-22T03:22:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-10-011310.3389/fmicb.2022.10409321040932Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamineMartha Patricia Chávez-Moctezuma0Ramiro Martínez-Cámara1Ramiro Martínez-Cámara2Julie Hernández-Salmerón3Gabriel Moreno-Hagelsieb4Gustavo Santoyo5Eduardo Valencia-Cantero6Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MexicoInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MexicoTecnológico Nacional de México, Morelia, Michoacán, MexicoDepartment of Biology, Wilfrid Laurier University, Waterloo, ON, CanadaDepartment of Biology, Wilfrid Laurier University, Waterloo, ON, CanadaInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MexicoInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MexicoQuorum sensing (QS) is a bacterial cell-cell communication system with genetically regulated mechanisms dependent on cell density. Canonical QS systems in gram-negative bacteria possess an autoinducer synthase (LuxI family) and a transcriptional regulator (LuxR family) that respond to an autoinducer molecule. In Gram-positive bacteria, the LuxR transcriptional regulators “solo” (not associated with a LuxI homolog) may play key roles in intracellular communication. Arthrobacter sp. UMCV2 is an actinobacterium that promotes plant growth by emitting the volatile organic compound N, N-dimethylhexadecylamine (DMHDA). This compound induces iron deficiency, defense responses in plants, and swarming motility in Arthrobacter sp. UMCV2. In this study, the draft genome of this bacterium was assembled and compared with the genomes of type strains of the Arthrobacter genus, finding that it does not belong to any previously described species. Genome explorations also revealed the presence of 16 luxR-related genes, but no luxI homologs were discovered. Eleven of these sequences possess the LuxR characteristic DNA-binding domain with a helix-turn-helix motif and were designated as auto-inducer-related regulators (AirR). Four sequences possessed LuxR analogous domains and were designated as auto-inducer analogous regulators (AiaR). When swarming motility was induced with DMHDA, eight airR genes and two aiaR genes were upregulated. These results indicate that the expression of multiple luxR-related genes is induced in actinobacteria, such as Arthrobacter sp. UMCV2, by the action of the bacterial biocompound DMHDA when QS behavior is produced.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1040932/fullactinobacteriaLuxR solosdomain approachairR genesaiaR genesswarming motility |
| spellingShingle | Martha Patricia Chávez-Moctezuma Ramiro Martínez-Cámara Ramiro Martínez-Cámara Julie Hernández-Salmerón Gabriel Moreno-Hagelsieb Gustavo Santoyo Eduardo Valencia-Cantero Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine Frontiers in Microbiology actinobacteria LuxR solos domain approach airR genes aiaR genes swarming motility |
| title | Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine |
| title_full | Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine |
| title_fullStr | Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine |
| title_full_unstemmed | Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine |
| title_short | Comparative genomic and functional analysis of Arthrobacter sp. UMCV2 reveals the presence of luxR-related genes inducible by the biocompound N, N-dimethylhexadecilamine |
| title_sort | comparative genomic and functional analysis of arthrobacter sp umcv2 reveals the presence of luxr related genes inducible by the biocompound n n dimethylhexadecilamine |
| topic | actinobacteria LuxR solos domain approach airR genes aiaR genes swarming motility |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1040932/full |
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